Ferric ammonium citrate | CAS# 1185-57-5

MedKoo Cat#: 592821 | Name: Ferric ammonium citrate

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Ferric ammonium citrate is a biochemical.

Chemical Structure

Ferric ammonium citrate

CAS#1185-57-5

Theoretical Analysis

MedKoo Cat#: 592821

Name: Ferric ammonium citrate

CAS#: 1185-57-5

Chemical Formula: C24H32Fe3N3O28

Exact Mass: 977.9221

Molecular Weight: 978.05

Elemental Analysis: C, 29.47; H, 3.30; Fe, 17.13; N, 4.30; O, 45.80

Price and Availability

Size	Price	Availability
10g	USD 260.00	Ready to ship
25g	USD 450.00	Ready to ship
100g	USD 850.00	Ready to ship

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Synonym

Ferric ammonium citrate

IUPAC/Chemical Name

1,2,3-Propanetricarboxylic acid, 2-hydroxy-, ammonium iron(3+) salt

InChi Key

AUALKMYBYGCYNY-UHFFFAOYSA-E

InChi Code

InChI=1S/4C6H8O7.3Fe.3H3N/c4*7-3(8)1-6(13,5(11)12)2-4(9)10;;;;;;/h4*13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;;;3*1H3/q;;;;3*+3;;;/p-9

SMILES Code

O=C(CC(C([O-])=O)(O)CC([O-])=O)[O-].O=C(CC(C([O-])=O)(O)CC([O-])=O)[O-].O=C(CC(C([O-])=O)(O)CC([O-])=O)[O-].O=C(CC(C([O-])=O)(O)CC([O-])=O)[O-].[NH4+].[Fe+3].[NH4+].[Fe+3].[NH4+].[Fe+3]

Appearance

Solid powder

Purity

Reagent grade (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in water

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.03.00

More Info

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot#A20A03S18

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

TBD

In vitro activity:

P-gp protein expression was substantially reduced by 36 ± 10% in hCMEC/D3 cells treated with 250 μM FAC for 72 h (Fig. 2a/b). To establish whether this was associated with a concomitant reduction in P-gp gene transcription, RNA was isolated from cells at various timepoints after FAC (ferric ammonium citrate)-treatment was initiated (as indicated in Fig. 2c) and MDR1 mRNA levels subsequently determined using RT-qPCR. A significant reduction in MDR1 mRNA levels was only observed (16 ± 5%) after 72 h of exposure to FAC (Fig. 2c). Reference: Pharm Res. 2021 Jan;38(1):97-111. https://pubmed.ncbi.nlm.nih.gov/33532991/

In vivo activity:

TBD

Preparing Stock Solutions

The following data is based on the product molecular weight 978.05 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Newman SA, Pan Y, Short JL, Nicolazzo JA. Increasing Intracellular Levels of Iron with Ferric Ammonium Citrate Leads to Reduced P-glycoprotein Expression in Human Immortalised Brain Microvascular Endothelial Cells. Pharm Res. 2021 Jan;38(1):97-111. doi: 10.1007/s11095-021-03006-y. Epub 2021 Feb 2. PMID: 33532991. 2. Wang H, Li Z, Niu J, Xu Y, Ma L, Lu A, Wang X, Qian Z, Huang Z, Jin X, Leng Q, Wang J, Zhong J, Sun B, Meng G. Antiviral effects of ferric ammonium citrate. Cell Discov. 2018 Mar 27;4:14. doi: 10.1038/s41421-018-0013-6. PMID: 29619244; PMCID: PMC5871618.

In vitro protocol:

In vivo protocol:

TBD

1: Pogge DJ, Drewnoski ME, Hansen SL. Feeding ferric ammonium citrate to decrease the risk of sulfur toxicity: effects on trace mineral absorption and status of beef steers. J Anim Sci. 2014 Sep;92(9):4005-13. doi: 10.2527/jas.2014-7799. Epub 2014 Jul 8. PubMed PMID: 25006066. 2: Chavasit V, Porasuphatana S, Suthutvoravut U, Zeder C, Hurrell R. Iron bioavailability in 8-24-month-old Thai children from a micronutrient-fortified quick-cooking rice containing ferric ammonium citrate or a mixture of ferrous sulphate and ferric sodium ethylenediaminetetraacetic acid. Matern Child Nutr. 2015 Dec;11 Suppl 4:179-87. doi: 10.1111/mcn.12167. PubMed PMID: 25721887. 3: Pogge DJ, Drewnoski ME, Snider D, Rumbeiha WK, Hansen SL. Effect of ferric ammonium citrate in feedlot diets with varying dried distillers' grains inclusion on ruminal hydrogen sulfide concentrations and steer growth. J Anim Sci. 2016 Sep;94(9):3894-3901. doi: 10.2527/jas.2016-0657. PubMed PMID: 27898902. 4: Li SW, Liu CM, Guo J, Marcondes AM, Deeg J, Li X, Guan F. Iron overload induced by ferric ammonium citrate triggers reactive oxygen species-mediated apoptosis via both extrinsic and intrinsic pathways in human hepatic cells. Hum Exp Toxicol. 2016 Jun;35(6):598-607. doi: 10.1177/0960327115597312. Epub 2015 Jul 29. PubMed PMID: 26224043. 5: Goralska M, Harned J, Fleisher LN, McGahan MC. The effect of ascorbic acid and ferric ammonium citrate on iron uptake and storage in lens epithelial cells. Exp Eye Res. 1998 Jun;66(6):687-97. PubMed PMID: 9657901. 6: Drewnoski ME, Doane P, Hansen SL. Ferric citrate decreases ruminal hydrogen sulphide concentrations in feedlot cattle fed diets high in sulphate. Br J Nutr. 2014 Jan 28;111(2):261-9. doi: 10.1017/S0007114513002304. Epub 2013 Jul 23. PubMed PMID: 23880397. 7: Takahashi T, Kato K, Nishizawa T, Nakazawa Y. [Elimination of bowel fluid artifact on abdominal DWI using oral contrast agent ferric ammonium citrate]. Nihon Hoshasen Gijutsu Gakkai Zasshi. 2008 May 20;64(5):573-8. Japanese. PubMed PMID: 18509218. 8: Kivelitz D, Gehl HB, Heuck A, Krahe T, Taupitz M, Lodemann KP, Hamm B. Ferric ammonium citrate as a positive bowel contrast agent for MR imaging of the upper abdomen. Safety and diagnostic efficacy. Acta Radiol. 1999 Jul;40(4):429-35. PubMed PMID: 10394873. 9: Szentmihályi A, Lányi B. Ferric ammonium citrate decomposition--a taxonomic tool for gram-negative bacteria. Acta Microbiol Hung. 1986;33(1):85-95. PubMed PMID: 3529797. 10: Dawson DJ, Jennis F. Mycobacteria with a growth requirement for ferric ammonium citrate, identified as Mycobacterium haemophilum. J Clin Microbiol. 1980 Feb;11(2):190-2. PubMed PMID: 7358843; PubMed Central PMCID: PMC273352. 11: Hirohashi S, Uchida H, Yoshikawa K, Fujita N, Ohtomo K, Yuasa Y, Kawamura Y, Matsui O. Large scale clinical evaluation of bowel contrast agent containing ferric ammonium citrate in MRI. Magn Reson Imaging. 1994;12(6):837-46. PubMed PMID: 7968283. 12: Richardson D, Baker E. Two mechanisms of iron uptake from transferrin by melanoma cells. The effect of desferrioxamine and ferric ammonium citrate. J Biol Chem. 1992 Jul 15;267(20):13972-9. PubMed PMID: 1629195. 13: Richardson DR, Baker E. The effect of desferrioxamine and ferric ammonium citrate on the uptake of iron by the membrane iron-binding component of human melanoma cells. Biochim Biophys Acta. 1992 Jan 31;1103(2):275-80. PubMed PMID: 1543713. 14: Guo X, Zheng H, Guo Y, Wang Y, Anderson GJ, Ci Y, Yu P, Geng L, Chang YZ. Nasal delivery of nanoliposome-encapsulated ferric ammonium citrate can increase the iron content of rat brain. J Nanobiotechnology. 2017 Jun 2;15(1):42. doi: 10.1186/s12951-017-0277-2. PubMed PMID: 28578696; PubMed Central PMCID: PMC5457662. 15: Wang EW, Agostini G, Olomu O, Runco D, Jung JY, Chole RA. Gentian violet and ferric ammonium citrate disrupt Pseudomonas aeruginosa biofilms. Laryngoscope. 2008 Nov;118(11):2050-6. doi: 10.1097/MLG.0b013e3181826e24. PubMed PMID: 18849857. 16: Finelli C, Giudice V, Vianelli N. [Ferritin and ammonium-ferric citrate. Considerations on absorption modality and therapeutic effectiveness]. G Clin Med. 1986 Jun;67(3):145-9. Italian. PubMed PMID: 3792721. 17: Yang P, Zhang YD, Li NF, Li WZ, Zhou GF. [Oral ferric ammonium citrate solution in MRCP for diagnosing low-level obstructive jaundice]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Apr;32(2):347-50. Chinese. PubMed PMID: 17478951. 18: Dykes GA, Dworaczek M. Influence of interactions between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in a defined medium. Lett Appl Microbiol. 2002;35(6):538-42. PubMed PMID: 12460440. 19: Mukherjee S, Murphy RA, Wawszkiewcz EJ. Effect of hemoglobin and of ferric ammonium citrate on the virulence of periodontopathic bacteria. Oral Microbiol Immunol. 1988 Dec;3(4):192-5. PubMed PMID: 3254476. 20: Taniguchi M, Imamura H, Shirota T, Okamatsu H, Fujii Y, Toba M, Hashimoto F. Improvement in iron deficiency anemia through therapy with ferric ammonium citrate and vitamin C and the effects of aerobic exercise. J Nutr Sci Vitaminol (Tokyo). 1991 Apr;37(2):161-71. PubMed PMID: 1919803.