MedKoo Cat#: 563618 | Name: NE100 HCl
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

NE100 HCl is a potent and selective sigma-1 receptor antagonist.

Chemical Structure

NE100 HCl
NE100 HCl
CAS#149409-57-4

Theoretical Analysis

MedKoo Cat#: 563618

Name: NE100 HCl

CAS#: 149409-57-4

Chemical Formula: C23H34ClNO2

Exact Mass: 391.2278

Molecular Weight: 391.98

Elemental Analysis: C, 70.48; H, 8.74; Cl, 9.04; N, 3.57; O, 8.16

Price and Availability

Size Price Availability Quantity
1mg USD 200.00
5mg USD 380.00
10mg USD 570.00
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Synonym
NE100 Hydrochloride; NE-100 Hydrochloride; NE 100 Hydrochloride; NE100 HCl; NE-100 HCl; NE 100 HCl;
IUPAC/Chemical Name
N,N-Di-n-propyl-2-[4-methoxy-3-(2-phenylethoxy)phenyl] ethylamine hydrochloride
InChi Key
ZHGMDXSHODHWHV-UHFFFAOYSA-N
InChi Code
InChI=1S/C23H33NO2.ClH/c1-4-15-24(16-5-2)17-13-21-11-12-22(25-3)23(19-21)26-18-14-20-9-7-6-8-10-20;/h6-12,19H,4-5,13-18H2,1-3H3;1H
SMILES Code
COC1=CC=C(CCN(CCC)CCC)C=C1OCCC2=CC=CC=C2.[H]Cl
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Biological target:
NE-100 (hydrochloride) is a potent and selective sigma-1 receptor antagonist with an IC50 of 4.16 nM.
In vitro activity:
The present study investigated whether NE-100 might suppress neuronal cell death that is induced by ER stress in ischemic injury. These studies show that NE-100 protected the ER stress-induced cell death of murine hippocampal HT22 cells, but not the oxidative stress-induced cell death. This suggests that NE-100 may have a protective effect on the ER. However, another sigma-1 receptor antagonist (BD1047) did not suppress ER stress-induced cell death. In addition, NE-100 attenuated the upregulation of C/EBP homologous protein (CHOP) induced by ER stress and upregulated the expression of both the 50-kDa activating transcription factor 6 (p50ATF6) and the 78-kDa glucose-regulated protein (GRP78). Reference: Biochem Biophys Res Commun. 2013 May 17;434(4):904-9. https://pubmed.ncbi.nlm.nih.gov/23618865/
In vivo activity:
This study investigated the effect of repeated inhibition of σ1R in the NAc on depressive-like behaviors and synaptic plasticity by microinjecting σ1R antagonist NE-100 into NAc nuclei in mice (NE-100 mice); this was followed by behavioral tests and field potentials recordings. Compared to control mice, NE-100 mice exhibited significantly prolonged immobility in forced swim test (FST) and tail suspension test (TST), impaired long-term depression (LTD) as well as multi-spike waveform field excitatory postsynaptic potential (fEPSP) with an extended duration and an increased paired-pulse ratio (PPR). Reduced levels of GABAA receptor (GABAAR)-α1, -α2, -β2, and -β3 subunits, membrane D2R, and PKC phosphorylation in the NAc were observed in NE-100 mice. Reference: Front Mol Neurosci. 2022 Sep 30;15:959224. https://pubmed.ncbi.nlm.nih.gov/36245919/
Solvent mg/mL mM
Solubility
DMF 30.0 76.53
DMSO 106.4 271.44
Ethanol 30.0 76.53
PBS (pH 7.2) 0.3 0.64
Water 54.9 140.06
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 391.98 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Matsushima Y, Terada K, Takata J, Karube Y, Kamei C, Sugimoto Y. Effects of fluvoxamine on nerve growth factor-induced neurite outgrowth inhibition by dexamethasone in PC12 cells. Biosci Biotechnol Biochem. 2019 Apr;83(4):659-665. doi: 10.1080/09168451.2018.1553607. Epub 2018 Dec 13. PMID: 30543144. 2. Ono Y, Tanaka H, Tsuruma K, Shimazawa M, Hara H. A sigma-1 receptor antagonist (NE-100) prevents tunicamycin-induced cell death via GRP78 induction in hippocampal cells. Biochem Biophys Res Commun. 2013 May 17;434(4):904-9. doi: 10.1016/j.bbrc.2013.04.055. Epub 2013 Apr 22. PMID: 23618865. 3. Qin Y, Xu W, Li K, Luo Q, Chen X, Wang Y, Chen L, Sha S. Repeated inhibition of sigma-1 receptor suppresses GABAA receptor expression and long-term depression in the nucleus accumbens leading to depressive-like behaviors. Front Mol Neurosci. 2022 Sep 30;15:959224. doi: 10.3389/fnmol.2022.959224. PMID: 36245919; PMCID: PMC9563353. 4. Vavers E, Zvejniece B, Stelfa G, Svalbe B, Vilks K, Kupats E, Mezapuke R, Lauberte L, Dambrova M, Zvejniece L. Genetic inactivation of the sigma-1 chaperone protein results in decreased expression of the R2 subunit of the GABA-B receptor and increased susceptibility to seizures. Neurobiol Dis. 2021 Mar;150:105244. doi: 10.1016/j.nbd.2020.105244. Epub 2020 Dec 30. PMID: 33385516.
In vitro protocol:
1. Matsushima Y, Terada K, Takata J, Karube Y, Kamei C, Sugimoto Y. Effects of fluvoxamine on nerve growth factor-induced neurite outgrowth inhibition by dexamethasone in PC12 cells. Biosci Biotechnol Biochem. 2019 Apr;83(4):659-665. doi: 10.1080/09168451.2018.1553607. Epub 2018 Dec 13. PMID: 30543144. 2. Ono Y, Tanaka H, Tsuruma K, Shimazawa M, Hara H. A sigma-1 receptor antagonist (NE-100) prevents tunicamycin-induced cell death via GRP78 induction in hippocampal cells. Biochem Biophys Res Commun. 2013 May 17;434(4):904-9. doi: 10.1016/j.bbrc.2013.04.055. Epub 2013 Apr 22. PMID: 23618865.
In vivo protocol:
1. Qin Y, Xu W, Li K, Luo Q, Chen X, Wang Y, Chen L, Sha S. Repeated inhibition of sigma-1 receptor suppresses GABAA receptor expression and long-term depression in the nucleus accumbens leading to depressive-like behaviors. Front Mol Neurosci. 2022 Sep 30;15:959224. doi: 10.3389/fnmol.2022.959224. PMID: 36245919; PMCID: PMC9563353. 2. Vavers E, Zvejniece B, Stelfa G, Svalbe B, Vilks K, Kupats E, Mezapuke R, Lauberte L, Dambrova M, Zvejniece L. Genetic inactivation of the sigma-1 chaperone protein results in decreased expression of the R2 subunit of the GABA-B receptor and increased susceptibility to seizures. Neurobiol Dis. 2021 Mar;150:105244. doi: 10.1016/j.nbd.2020.105244. Epub 2020 Dec 30. PMID: 33385516.
1: Li D, Zhang SZ, Yao YH, Xiang Y, Ma XY, Wei XL, Yan HT, Liu XY. Sigma-1 receptor agonist increases axon outgrowth of hippocampal neurons via voltage-gated calcium ions channels. CNS Neurosci Ther. 2017 Dec;23(12):930-939. doi: 10.1111/cns.12768. Epub 2017 Oct 8. PubMed PMID: 28990373. 2: Di T, Zhang S, Hong J, Zhang T, Chen L. Hyperactivity of Hypothalamic-Pituitary-Adrenal Axis Due to Dysfunction of the Hypothalamic Glucocorticoid Receptor in Sigma-1 Receptor Knockout Mice. Front Mol Neurosci. 2017 Sep 6;10:287. doi: 10.3389/fnmol.2017.00287. eCollection 2017. PubMed PMID: 28932185; PubMed Central PMCID: PMC5592243. 3: Chen T, Tanaka M, Wang Y, Sha S, Furuya K, Chen L, Sokabe M. Neurosteroid dehydroepiandrosterone enhances activity and trafficking of astrocytic GLT-1 via σ(1) receptor-mediated PKC activation in the hippocampal dentate gyrus of rats. Glia. 2017 Sep;65(9):1491-1503. doi: 10.1002/glia.23175. Epub 2017 Jun 5. PubMed PMID: 28581152. 4: Lenart L, Hodrea J, Hosszu A, Koszegi S, Zelena D, Balogh D, Szkibinszkij E, Veres-Szekely A, Wagner L, Vannay A, Szabo AJ, Fekete A. The role of sigma-1 receptor and brain-derived neurotrophic factor in the development of diabetes and comorbid depression in streptozotocin-induced diabetic rats. Psychopharmacology (Berl). 2016 Apr;233(7):1269-78. doi: 10.1007/s00213-016-4209-x. Epub 2016 Jan 26. PubMed PMID: 26809458. 5: Tanimukai H, Kudo T. Fluvoxamine alleviates paclitaxel-induced neurotoxicity. Biochem Biophys Rep. 2015 Sep 25;4:202-206. doi: 10.1016/j.bbrep.2015.09.014. eCollection 2015 Dec. PubMed PMID: 29124205; PubMed Central PMCID: PMC5668922. 6: Hong J, Sha S, Zhou L, Wang C, Yin J, Chen L. Sigma-1 receptor deficiency reduces MPTP-induced parkinsonism and death of dopaminergic neurons. Cell Death Dis. 2015 Jul 23;6:e1832. doi: 10.1038/cddis.2015.194. PubMed PMID: 26203861; PubMed Central PMCID: PMC4650739. 7: Yin J, Sha S, Chen T, Wang C, Hong J, Jie P, Zhou R, Li L, Sokabe M, Chen L. Sigma-1 (σ₁) receptor deficiency reduces β-amyloid(25-35)-induced hippocampal neuronal cell death and cognitive deficits through suppressing phosphorylation of the NMDA receptor NR2B. Neuropharmacology. 2015 Feb;89:215-24. doi: 10.1016/j.neuropharm.2014.09.027. Epub 2014 Oct 5. PubMed PMID: 25286118. 8: Sánchez-Blázquez P, Rodríguez-Muñoz M, Herrero-Labrador R, Burgueño J, Zamanillo D, Garzón J. The calcium-sensitive Sigma-1 receptor prevents cannabinoids from provoking glutamate NMDA receptor hypofunction: implications in antinociception and psychotic diseases. Int J Neuropsychopharmacol. 2014 Dec;17(12):1943-55. doi: 10.1017/S1461145714000029. Epub 2014 Jan 31. PubMed PMID: 24485144. 9: Yang R, Chen L, Wang H, Xu B, Tomimoto H, Chen L. Anti-amnesic effect of neurosteroid PREGS in Aβ25-35-injected mice through σ1 receptor- and α7nAChR-mediated neuroprotection. Neuropharmacology. 2012 Nov;63(6):1042-50. doi: 10.1016/j.neuropharm.2012.07.035. Epub 2012 Jul 31. PubMed PMID: 22884465. 10: Li L, Xu B, Zhu Y, Chen L, Sokabe M, Chen L. DHEA prevents Aβ25-35-impaired survival of newborn neurons in the dentate gyrus through a modulation of PI3K-Akt-mTOR signaling. Neuropharmacology. 2010 Sep-Oct;59(4-5):323-33. doi: 10.1016/j.neuropharm.2010.02.009. Epub 2010 Feb 16. PubMed PMID: 20167228. 11: Li Z, Cui S, Zhang Z, Zhou R, Ge Y, Sokabe M, Chen L. DHEA-neuroprotection and -neurotoxicity after transient cerebral ischemia in rats. J Cereb Blood Flow Metab. 2009 Feb;29(2):287-96. doi: 10.1038/jcbfm.2008.118. Epub 2008 Oct 15. PubMed PMID: 18854841. 12: Cai W, Zhu Y, Furuya K, Li Z, Sokabe M, Chen L. Two different molecular mechanisms underlying progesterone neuroprotection against ischemic brain damage. Neuropharmacology. 2008 Aug;55(2):127-38. doi: 10.1016/j.neuropharm.2008.04.023. Epub 2008 May 10. PubMed PMID: 18572204. 13: Li Z, Zhou R, Cui S, Xie G, Cai W, Sokabe M, Chen L. Dehydroepiandrosterone sulfate prevents ischemia-induced impairment of long-term potentiation in rat hippocampal CA1 by up-regulating tyrosine phosphorylation of NMDA receptor. Neuropharmacology. 2006 Oct;51(5):958-66. Epub 2006 Aug 8. PubMed PMID: 16895729. 14: Chen L, Dai XN, Sokabe M. Chronic administration of dehydroepiandrosterone sulfate (DHEAS) primes for facilitated induction of long-term potentiation via sigma 1 (sigma1) receptor: optical imaging study in rat hippocampal slices. Neuropharmacology. 2006 Mar;50(3):380-92. Epub 2005 Dec 20. PubMed PMID: 16364377. 15: Colabufo NA, Berardi F, Contino M, Niso M, Abate C, Perrone R, Tortorella V. Antiproliferative and cytotoxic effects of some sigma2 agonists and sigma1 antagonists in tumour cell lines. Naunyn Schmiedebergs Arch Pharmacol. 2004 Aug;370(2):106-13. Epub 2004 Jul 31. PubMed PMID: 15322732. 16: Takebayashi M, Hayashi T, Su TP. Nerve growth factor-induced neurite sprouting in PC12 cells involves sigma-1 receptors: implications for antidepressants. J Pharmacol Exp Ther. 2002 Dec;303(3):1227-37. PubMed PMID: 12438547.