Synonym
S 2484; S-2484; S2484; Pyro-glu-pro-val-pna;
IUPAC/Chemical Name
(S)-4-amino-5-((S)-2-(((S)-1-amino-3-methyl-1-oxobutan-2-yl)(4-nitrophenyl)carbamoyl)pyrrolidin-1-yl)-5-oxopentanoic acid
InChi Key
WPSYDPBAHWFTEJ-BQFCYCMXSA-N
InChi Code
InChI=1S/C21H29N5O7/c1-12(2)18(19(23)29)25(13-5-7-14(8-6-13)26(32)33)21(31)16-4-3-11-24(16)20(30)15(22)9-10-17(27)28/h5-8,12,15-16,18H,3-4,9-11,22H2,1-2H3,(H2,23,29)(H,27,28)/t15-,16-,18-/m0/s1
SMILES Code
CC(C)[C@@H](C(N)=O)N(C([C@H]1N(C([C@H](CCC(O)=O)N)=O)CCC1)=O)C2=CC=C([N+]([O-])=O)C=C2
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
Preparing Stock Solutions
The following data is based on the
product
molecular weight
463.49
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Tanaka H, Shimazu T, Sugimoto H, Yoshioka T, Sugimoto T. A sensitive and specific assay for granulocyte elastase in inflammatory tissue fluid using L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide. Clin Chim Acta. 1990 Feb 28;187(2):173-80. PubMed PMID: 2317934.
2: Hamanaka K, Toki N, Takasugi S, Sumi H. Effect of plant SH-protease on human polymorphonuclear leukocyte granule elastase. J Dermatol. 1984 Dec;11(6):523-8. PubMed PMID: 6399052.
3: Regan J, McGarry D, Bruno J, Green D, Newman J, Hsu CY, Kline J, Barton J, Travis J, Choi YM, Volz F, Pauls H, Harrison R, Zilberstein A, Ben-Sasson SA, Chang M. Anionic- and lipophilic-mediated surface binding inhibitors of human leukocyte elastase. J Med Chem. 1997 Oct 10;40(21):3408-22. PubMed PMID: 9341916.
4: Figueredo CM, Gustafsson A, Asman B, Bergström K. Increased release of elastase from in vitro activated peripheral neutrophils in patients with adult periodontitis. J Clin Periodontol. 1999 Apr;26(4):206-11. PubMed PMID: 10223390.
5: Kramps JA, van Twisk C, van der Linden AC. L-Pyroglutamyl-L-prolyl-L-valine-p-nitroanilide, a highly specific substrate for granulocyte elastase. Scand J Clin Lab Invest. 1983 Sep;43(5):427-32. PubMed PMID: 6557666.
6: Kramps JA, Morrison HM, Burnett D, Dijkman JH, Stockley RA. Determination of elastase inhibitory activity of alpha 1-proteinase inhibitor and bronchial antileukoprotease: different results using insoluble elastin or synthetic low molecular weight substrates. Scand J Clin Lab Invest. 1987 Jun;47(4):405-10. PubMed PMID: 2440098.
