Pyronin Y | CAS# 92-32-0 | biological stain

MedKoo Cat#: 592290 | Name: Pyronin Y

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Pyronine is a Xanthene dye used as a bacterial and biological stain.

Chemical Structure

Pyronin Y

CAS# 92-32-0

Theoretical Analysis

MedKoo Cat#: 592290

Name: Pyronin Y

CAS#: 92-32-0

Chemical Formula: C17H19ClN2O

Exact Mass:

Molecular Weight: 302.80

Elemental Analysis: C, 67.43; H, 6.32; Cl, 11.71; N, 9.25; O, 5.28

Price and Availability

Size	Price	Availability	Quantity
1g	USD 230.00
5g	USD 360.00
10g	USD 560.00

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Related CAS #

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Synonym

Pyronine; Pyronin Y

IUPAC/Chemical Name

Ammonium, (6-(dimethylamino)-3H-xanten-3-ylidene)dimethyl-, chloride

InChi Key

INCIMLINXXICKS-UHFFFAOYSA-M

InChi Code

InChI=1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

SMILES Code

C/[N+](C)=C1C=CC2=CC3=C(C=C(N(C)C)C=C3)OC2=C/1.[Cl-]

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.03.00

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Pyronin Y is a dye.

In vitro activity:

In this study, pyronin Y was used as a staining agent for cellular RNA in Chinese hamster ovary (CHO) cells. Pyronin Y was used to assess the stainability of RNA in different cellular conditions, such as mitosis (M), G2 phase, and interphase cells exposed to hyperthermia. Pyronin Y was used to as a sensitive probe to investigate the effects of mitosis and hyperthermia on the stainability of RNA in CHO cells. Reference: Exp Cell Res. 1988 Dec;179(2):535-44. https://pubmed.ncbi.nlm.nih.gov/2461311/

In vivo activity:

Pyronin assessed primitive hematopoietic stem cells (PHSC) during G(0) and G(1). It differentiated the Rh(lo)Lin(-)Kit(+)Sca-1(+) PHSC subset from more mature cells. Further, it revealed diverse functionality within the Rh(lo)Ho(lo/sp)Lin(-)Sca-1(+) PHSC group, with PY(lo)Rh(lo)Ho(lo/sp) PHSC having a higher competitive repopulating unit (CRU) frequency than PY(hi) PHSC at G(1). Pyronin showed dose-dependent cytotoxicity, impacting non-early hematopoietic cells while sparing the early HSC compartment. Reference: Exp Hematol. 2001 Sep;29(9):1109-16. https://pubmed.ncbi.nlm.nih.gov/11532352/

	Solvent	mg/mL	mM
Solubility
	DMSO	25.0	82.56
	Water	4.0	13.21

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 302.80 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Salci A, Toprak M. Spectroscopic investigations on the binding of Pyronin Y to human serum albumin. J Biomol Struct Dyn. 2017 Jan;35(1):8-16. doi: 10.1080/07391102.2015.1128357. Epub 2016 Feb 17. PMID: 26646531. 2. Traganos F, Crissman HA, Darzynkiewicz Z. Staining with pyronin Y detects changes in conformation of RNA during mitosis and hyperthermia of CHO cells. Exp Cell Res. 1988 Dec;179(2):535-44. doi: 10.1016/0014-4827(88)90291-1. PMID: 2461311. In vivo study 1. Hüttmann A, Liu SL, Boyd AW, Li CL. Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y. Exp Hematol. 2001 Sep;29(9):1109-16. doi: 10.1016/s0301-472x(01)00684-1. PMID: 11532352.

In vitro protocol:

In vivo protocol:

1. Hüttmann A, Liu SL, Boyd AW, Li CL. Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y. Exp Hematol. 2001 Sep;29(9):1109-16. doi: 10.1016/s0301-472x(01)00684-1. PMID: 11532352.