IMM-01 | CAS#218795-74-5 | DID-DAD inhibitor

MedKoo Cat#: 563309 | Name: IMM-01

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

IMM-01 is an inhibitor of DID-DAD binding.

Chemical Structure

IMM-01

CAS#218795-74-5

Theoretical Analysis

MedKoo Cat#: 563309

Name: IMM-01

CAS#: 218795-74-5

Chemical Formula: C12H17N3O2S

Exact Mass: 267.1041

Molecular Weight: 267.35

Elemental Analysis: C, 53.91; H, 6.41; N, 15.72; O, 11.97; S, 11.99

Price and Availability

Size	Price	Availability	Quantity
10mg	USD 350.00	2 Weeks
50mg	USD 950.00	2 Weeks

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Synonym

IMM-01; IMM 01; IMM01;

IUPAC/Chemical Name

N1-(tert-butyl)-2-(2,4-dihydroxybenzylidene)hydrazine-1-carbothioamide

InChi Key

LTFUAYRGVLQXKC-NTUHNPAUSA-N

InChi Code

InChI=1S/C12H17N3O2S/c1-12(2,3)14-11(18)15-13-7-8-4-5-9(16)6-10(8)17/h4-7,16-17H,1-3H3,(H2,14,15,18)/b13-7+

SMILES Code

S=C(N/N=C/C1=CC=C(O)C=C1O)NC(C)(C)C

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.03.00

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Product Data

Product Data

Instruction

View handling instruction

Biological target:

IMM-01 is an inhibitor of DID-DAD binding.

In vitro activity:

This data demonstrated crystal structure of the IMM01/CD47 complex and provides a structural basis for the structural binding interface and future clinical applications. Reference: Molecules. 2022 Aug 30;27(17):5574. https://pubmed.ncbi.nlm.nih.gov/36080360/

In vivo activity:

Conversely, IMM and mDia2 DAD peptides were minimally toxic at concentrations up to 10-20 and 50 μM, respectively. SMIFH2's therapeutic potential may therefore be limited by its substantial in vivo toxicity at functional concentrations. mDia formin agonism with IMMs and mDia2 DADs may therefore be a more effective and less toxic anti-invasive therapeutic approach. Reference: Front Pharmacol. 2018 Apr 10;9:340. https://pubmed.ncbi.nlm.nih.gov/29692731/

Preparing Stock Solutions

The following data is based on the product molecular weight 267.35 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Yu J, Li S, Chen D, Liu D, Guo H, Yang C, Zhang W, Zhang L, Zhao G, Tu X, Peng L, Liu S, Bai X, Song Y, Jiang Z, Zhang R, Tian W. Crystal Structure of Human CD47 in Complex with Engineered SIRPα.D1(N80A). Molecules. 2022 Aug 30;27(17):5574. doi: 10.3390/molecules27175574. PMID: 36080360; PMCID: PMC9457805. 2. Schneider R, Deutsch K, Hoeprich GJ, Marquez J, Hermle T, Braun DA, Seltzsam S, Kitzler TM, Mao Y, Buerger F, Majmundar AJ, Onuchic-Whitford AC, Kolvenbach CM, Schierbaum L, Schneider S, Halawi AA, Nakayama M, Mann N, Connaughton DM, Klämbt V, Wagner M, Riedhammer KM, Renders L, Katsura Y, Thumkeo D, Soliman NA, Mane S, Lifton RP, Shril S, Khokha MK, Hoefele J, Goode BL, Hildebrandt F. DAAM2 Variants Cause Nephrotic Syndrome via Actin Dysregulation. Am J Hum Genet. 2020 Dec 3;107(6):1113-1128. doi: 10.1016/j.ajhg.2020.11.008. Epub 2020 Nov 23. PMID: 33232676; PMCID: PMC7820625. 3. LeCorgne H, Tudosie AM, Lavik K, Su R, Becker KN, Moore S, Walia Y, Wisner A, Koehler D, Alberts AS, Williams FE, Eisenmann KM. Differential Toxicity of mDia Formin-Directed Functional Agonists and Antagonists in Developing Zebrafish. Front Pharmacol. 2018 Apr 10;9:340. doi: 10.3389/fphar.2018.00340. PMID: 29692731; PMCID: PMC5902741.

In vitro protocol:

In vivo protocol:

1. LeCorgne H, Tudosie AM, Lavik K, Su R, Becker KN, Moore S, Walia Y, Wisner A, Koehler D, Alberts AS, Williams FE, Eisenmann KM. Differential Toxicity of mDia Formin-Directed Functional Agonists and Antagonists in Developing Zebrafish. Front Pharmacol. 2018 Apr 10;9:340. doi: 10.3389/fphar.2018.00340. PMID: 29692731; PMCID: PMC5902741.

1: Arden JD, Lavik KI, Rubinic KA, Chiaia N, Khuder SA, Howard MJ, Nestor-Kalinoski AL, Alberts AS, Eisenmann KM. Small-molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy. Mol Biol Cell. 2015 Nov 1;26(21):3704-18. doi: 10.1091/mbc.E14-11-1502. Epub 2015 Sep 9. PubMed PMID: 26354425; PubMed Central PMCID: PMC4626057. 2: Lash LL, Wallar BJ, Turner JD, Vroegop SM, Kilkuskie RE, Kitchen-Goosen SM, Xu HE, Alberts AS. Small-molecule intramimics of formin autoinhibition: a new strategy to target the cytoskeletal remodeling machinery in cancer cells. Cancer Res. 2013 Nov 15;73(22):6793-803. doi: 10.1158/0008-5472.CAN-13-1593. PubMed PMID: 24242070.