MedKoo Cat#: 558164 | Name: Toluidine Green

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Toluidine Green is a stain.

Chemical Structure

Toluidine Green
CAS#4430-16-4

Theoretical Analysis

MedKoo Cat#: 558164

Name: Toluidine Green

CAS#: 4430-16-4

Chemical Formula: C28H20N2Na2O10S2

Exact Mass: 654.0400

Molecular Weight: 654.57

Elemental Analysis: C, 51.38; H, 3.08; N, 4.28; Na, 7.02; O, 24.44; S, 9.80

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.
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Synonym
Toluidine Green; Alizarine Brilliant Green 5G; Alizarine Cyanine Green 5G; Alizarine viridine;
IUPAC/Chemical Name
Benzenesulfonic acid, 2,2'-((9,10-dihydro-5,8-dihydroxy-9,10-dioxo-1,4-anthracenediyl)diimino)bis(5-methyl-, disodium salt
InChi Key
LERIXBKRKBHLHG-UHFFFAOYSA-L
InChi Code
InChI=1S/C28H22N2O10S2.2Na/c1-13-3-5-15(21(11-13)41(35,36)37)29-17-7-8-18(30-16-6-4-14(2)12-22(16)42(38,39)40)24-23(17)27(33)25-19(31)9-10-20(32)26(25)28(24)34;;/h3-12,29-32H,1-2H3,(H,35,36,37)(H,38,39,40);;/q;2*+1/p-2
SMILES Code
O=C(C1=C2C(O)=CC=C1O)C3=C(NC4=CC=C(C)C=C4S(=O)([O-])=O)C=CC(NC5=CC=C(C)C=C5S(=O)([O-])=O)=C3C2=O.[Na+].[Na+]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 654.57 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Shapiro HM, Schildkraut ER, Curbelo R, Laird CW, Turner B, Hirschfeld T. Combined blood cell counting and classification with fluorochrome stains and flow instrumentation. J Histochem Cytochem. 1976 Jan;24(1):396-401. PubMed PMID: 56391. 2: Curtis SK, Cowden RR. Effects of preparation and fixation on three quantitative fluorescent cytochemical procedures. Histochemistry. 1980;68(1):29-38. PubMed PMID: 6157658. 3: Curtis SK, Cowden RR. Microfluorometric estimates of proteins associated with murine hepatocyte and thymocyte nuclei, residual structures, and nuclear matrix derivatives. Histochemistry. 1985;82(4):331-9. PubMed PMID: 4008276. 4: Freeman DA, Crissman HA. Evaluation of six fluorescent protein stains for use in flow microfluorometry. Stain Technol. 1975 Jul;50(4):279-84. PubMed PMID: 52920. 5: Aeby P, Schultze U, Braichotte D, Bundt M, Moser-Boroumand F, Wydler H, Fluhler H. Fluorescence imaging of tracer distributions in soil profiles. Environ Sci Technol. 2001 Feb 15;35(4):753-60. PubMed PMID: 11349288. 6: Oheim M, Naraghi M, Müller TH, Neher E. Two dye two wavelength excitation calcium imaging: results from bovine adrenal chromaffin cells. Cell Calcium. 1998 Jul;24(1):71-84. PubMed PMID: 9793690. 7: Cowden RR, Curtis SK. Microfluorometric investigations of chromatin structure. IV. Determination of total protein values in thymocyte and hepatocyte nuclei. Effects of extraction with 0.4 N H2SO4 and 0.35 M NaC1. Histochemistry. 1982;74(3):329-39. PubMed PMID: 7107322.