Toluidine Blue (also known as tolonium chloride or Toluidine blue O) is metachromatic dye used for a variety of histological staining, it selectively stains acidic tissue components (sulfates, carboxylates, and phosphate radicals).Toluidine blue has an affinity for nucleic acids, and therefore binds to nuclear material of tissues with a high DNA and RNA content.
MedKoo Cat#: 126284
Name: Toluidine Blue
CAS#: 3209-30-1
Chemical Formula: C28H20N2Na2O10S2
Exact Mass: 654.0355
Molecular Weight: 654.57
Elemental Analysis: C, 51.38; H, 3.08; N, 4.28; Na, 7.02; O, 24.44; S, 9.80
The following data is based on the product molecular weight 654.57 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
