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MedKoo Cat#: 591519 | Name: Methyl Orange
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Methyl orange is a pH indicator frequently used in titration because of its clear and distinct colour variance at different pH values.

Chemical Structure

Methyl Orange
Methyl Orange
CAS#547-58-0

Theoretical Analysis

MedKoo Cat#: 591519

Name: Methyl Orange

CAS#: 547-58-0

Chemical Formula: C14H14N3NaO3S

Exact Mass: 327.0654

Molecular Weight: 327.33

Elemental Analysis: C, 51.37; H, 4.31; N, 12.84; Na, 7.02; O, 14.66; S, 9.79

Price and Availability

Size Price Availability Quantity
5g USD 250.00 2 weeks
25g USD 450.00 2 weeks
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Related CAS #
No Data
Synonym
Methyl Orange; NSC 9416; NSC9416; NSC-9416
IUPAC/Chemical Name
Benzenesulfonic acid, 4-((4-(dimethylamino)phenyl)azo)-, sodium salt
InChi Key
STZCRXQWRGQSJD-GEEYTBSJSA-M
InChi Code
InChI=1S/C14H15N3O3S.Na/c1-17(2)13-7-3-11(4-8-13)15-16-12-5-9-14(10-6-12)21(18,19)20;/h3-10H,1-2H3,(H,18,19,20);/q;+1/p-1/b16-15+;
SMILES Code
O=S(C1=CC=C(/N=N/C2=CC=C(N(C)C)C=C2)C=C1)([O-])=O.[Na+]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Methyl Orange is an azo dye and organic contaminant.
In vitro activity:
A novel pH optical sensor based on triacetylcellulose membrane as solid support was developed by using immobilization of methyl orange indicator. Determination of pH by using the proposed optical sensor is on-line, quick, inexpensive, selective and sensitive in the pH range of 4.0-12.0. Reference: Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15;128:864-7. https://pubmed.ncbi.nlm.nih.gov/24709351/
In vivo activity:
TBD
Solvent mg/mL mM comments
Solubility
DMSO 62.5 190.94
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 327.33 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Hosseini M, Heydari R, Alimoradi M. A novel pH optical sensor using methyl orange based on triacetylcellulose membranes as support. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15;128:864-7. doi: 10.1016/j.saa.2014.02.171. Epub 2014 Mar 19. PMID: 24709351. 2. Wang YH, Zhu YS, Zhu SX. [Effect of pH of methyl orange solution on the fluorescence enhancement effect of silver nanoparticles]. Guang Pu Xue Yu Guang Pu Fen Xi. 2011 May;31(5):1295-9. Chinese. PMID: 21800586.
In vitro protocol:
1. Hosseini M, Heydari R, Alimoradi M. A novel pH optical sensor using methyl orange based on triacetylcellulose membranes as support. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15;128:864-7. doi: 10.1016/j.saa.2014.02.171. Epub 2014 Mar 19. PMID: 24709351. 2. Wang YH, Zhu YS, Zhu SX. [Effect of pH of methyl orange solution on the fluorescence enhancement effect of silver nanoparticles]. Guang Pu Xue Yu Guang Pu Fen Xi. 2011 May;31(5):1295-9. Chinese. PMID: 21800586.
In vivo protocol:
TBD
1: Dragan ES, Dinu MV. Spectacular Selectivity in the Capture of Methyl Orange by Composite Anion Exchangers with the Organic Part Hosted by DAISOGEL Microspheres. ACS Appl Mater Interfaces. 2018 Jun 5. doi: 10.1021/acsami.8b04498. [Epub ahead of print] PubMed PMID: 29799721. 2: Shen Z, Zhou H, Chen H, Xu H, Feng C, Zhou X. Synthesis of Nano-Zinc Oxide Loaded on Mesoporous Silica by Coordination Effect and Its Photocatalytic Degradation Property of Methyl Orange. Nanomaterials (Basel). 2018 May 9;8(5). pii: E317. doi: 10.3390/nano8050317. PubMed PMID: 29747457; PubMed Central PMCID: PMC5977331. 3: Radini IA, Hasan N, Malik MA, Khan Z. Biosynthesis of iron nanoparticles using Trigonella foenum-graecum seed extract for photocatalytic methyl orange dye degradation and antibacterial applications. J Photochem Photobiol B. 2018 Jun;183:154-163. doi: 10.1016/j.jphotobiol.2018.04.014. Epub 2018 Apr 17. PubMed PMID: 29705508. 4: Huang T, Liu L, Tao J, Zhou L, Zhang S. Microbial fuel cells coupling with the three-dimensional electro-Fenton technique enhances the degradation of methyl orange in the wastewater. Environ Sci Pollut Res Int. 2018 Apr 23. doi: 10.1007/s11356-018-1976-4. [Epub ahead of print] PubMed PMID: 29687198. 5: Mohammed SA, Al Amouri L, Yousif E, Ali AA, Mabood F, Abbas HF, Alyaqoobi S. Synthesis of NiO:V(2)O(5) nanocomposite and its photocatalytic efficiency for methyl orange degradation. Heliyon. 2018 Mar 17;4(3):e00581. doi: 10.1016/j.heliyon.2018.e00581. eCollection 2018 Mar. PubMed PMID: 29687084; PubMed Central PMCID: PMC5904460. 6: Habiba U, Siddique TA, Li Lee JJ, Joo TC, Ang BC, Afifi AM. Adsorption study of methyl orange by chitosan/polyvinyl alcohol/zeolite electrospun composite nanofibrous membrane. Carbohydr Polym. 2018 Jul 1;191:79-85. doi: 10.1016/j.carbpol.2018.02.081. Epub 2018 Mar 7. PubMed PMID: 29661324. 7: Nasirian M, Mehrvar M. Photocatalytic degradation of aqueous Methyl Orange using nitrogen-doped TiO(2) photocatalyst prepared by novel method of ultraviolet-assisted thermal synthesis. J Environ Sci (China). 2018 Apr;66:81-93. doi: 10.1016/j.jes.2017.05.032. Epub 2017 May 27. PubMed PMID: 29628111. 8: Dan Z, Yang Y, Qin F, Wang H, Chang H. Facile Fabrication of Cu₂O Nanobelts in Ethanol on Nanoporous Cu and their Photodegradation of Methyl Orange. Materials (Basel). 2018 Mar 19;11(3). pii: E446. doi: 10.3390/ma11030446. PubMed PMID: 29562692; PubMed Central PMCID: PMC5873025. 9: Yu J, Zhang X, Wang D, Li P. Adsorption of methyl orange dye onto biochar adsorbent prepared from chicken manure. Water Sci Technol. 2018 Mar;77(5-6):1303-1312. doi: 10.2166/wst.2018.003. PubMed PMID: 29528318; PubMed Central PMCID: wst_2018_003. 10: Nançoz C, Licari G, Beckwith JS, Soederberg M, Dereka B, Rosspeintner A, Yushchenko O, Letrun R, Richert S, Lang B, Vauthey E. Influence of the hydrogen-bond interactions on the excited-state dynamics of a push-pull azobenzene dye: the case of Methyl Orange. Phys Chem Chem Phys. 2018 Mar 7;20(10):7254-7264. doi: 10.1039/c7cp08390d. PubMed PMID: 29484322. 11: Wang L, Zhang M, Huang Q, Zhao C, Luo K, Lei M. Fabrication of ACF/GO/PEI Composite for Adsorption of Methyl Orange from Aqueous Solution. J Nanosci Nanotechnol. 2018 Mar 1;18(3):1747-1756. doi: 10.1166/jnn.2018.14225. PubMed PMID: 29448654. 12: Fan JM, Zhao ZH, Gong C, Xue YQ, Yin S. Persistent Methyl Orange Degradation Ability of MgAl₂O₄:(Pr(3+),Dy(3+))/M-TiO₂ Luminescent Photocatalyst. J Nanosci Nanotechnol. 2018 Mar 1;18(3):1675-1681. doi: 10.1166/jnn.2018.14247. PubMed PMID: 29448644. 13: Guo S, Zhang J, Li X, Zhang F, Zhu X. Fe(3)O(4)-CS-L: a magnetic core-shell nano adsorbent for highly efficient methyl orange adsorption. Water Sci Technol. 2018 Feb;77(3):628-637. doi: 10.2166/wst.2017.602. PubMed PMID: 29431707; PubMed Central PMCID: wst_2017_602. 14: Li X, Yuan H, Quan X, Chen S, You S. Effective adsorption of sulfamethoxazole, bisphenol A and methyl orange on nanoporous carbon derived from metal-organic frameworks. J Environ Sci (China). 2018 Jan;63:250-259. doi: 10.1016/j.jes.2017.10.019. Epub 2017 Nov 10. PubMed PMID: 29406107. 15: Wu M, Han H, Ni L, Song D, Li S, Hu T, Jiang J, Chen J. Preparation of 1D Hierarchical Material Mesosilica/Pal Composite and Its Performance in the Adsorption of Methyl Orange. Materials (Basel). 2018 Jan 20;11(1). pii: E164. doi: 10.3390/ma11010164. PubMed PMID: 29361713; PubMed Central PMCID: PMC5793662. 16: Rashed MN, Eltaher MA, Abdou ANA. Adsorption and photocatalysis for methyl orange and Cd removal from wastewater using TiO(2)/sewage sludge-based activated carbon nanocomposites. R Soc Open Sci. 2017 Dec 13;4(12):170834. doi: 10.1098/rsos.170834. eCollection 2017 Dec. PubMed PMID: 29308227; PubMed Central PMCID: PMC5749995. 17: Arabi M, Baizaee SM, Bahador A, Otaqsara SMT. Rapid, controllable, one-pot and room-temperature aqueous synthesis of ZnO:Cu nanoparticles by pulsed UV laser and its application for photocatalytic degradation of methyl orange. Luminescence. 2018 May;33(3):475-485. doi: 10.1002/bio.3436. Epub 2017 Dec 28. PubMed PMID: 29282896. 18: Jiang Y, Liu B, Xu J, Pan K, Hou H, Hu J, Yang J. Cross-linked chitosan/β-cyclodextrin composite for selective removal of methyl orange: Adsorption performance and mechanism. Carbohydr Polym. 2018 Feb 15;182:106-114. doi: 10.1016/j.carbpol.2017.10.097. Epub 2017 Oct 31. PubMed PMID: 29279104. 19: Umamaheswari C, Lakshmanan A, Nagarajan NS. Green synthesis, characterization and catalytic degradation studies of gold nanoparticles against congo red and methyl orange. J Photochem Photobiol B. 2018 Jan;178:33-39. doi: 10.1016/j.jphotobiol.2017.10.017. Epub 2017 Oct 16. PubMed PMID: 29101871. 20: Maham M, Nasrollahzadeh M, Bagherzadeh M, Akbari R. Green Synthesis of Palladium/Titanium Dioxide Nanoparticles and their Application for the Reduction of Methyl Orange, Congo Red and Rhodamine B in Aqueous Medium. Comb Chem High Throughput Screen. 2017;20(9):787-795. doi: 10.2174/1386207320666171023154523. PubMed PMID: 29065826.