L-Leucine-1-13C | CAS#74292-94-7 | essential amino acid

MedKoo Cat#: 596455 | Name: L-Leucine-1-13C

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

L-Leucine-1-13C is an essential amino acid that is used in the biosynthesis of proteins. Leucine is an α-amino acid, meaning it contains an α-amino group (which is in the protonated −NH3+ form under biological conditions), an α-carboxylic acid group (which is in the deprotonated −COO− form under biological conditions), and a side chain isobutyl group, making it a non-polar aliphatic amino acid. It is essential in humans, meaning the body cannot synthesize it: it must be obtained from the diet. Human dietary sources are foods that contain protein, such as meats, dairy products, soy products, and beans and other legumes. In the genetic code, it is encoded by the six codons UUA, UUG, CUU, CUC, CUA, and CUG. Like valine and isoleucine, leucine is a branched-chain amino acid. The primary metabolic end products of leucine metabolism are acetyl-CoA and acetoacetate; consequently, it is one of the two exclusively ketogenic amino acids, with lysine being the other. It is the most important ketogenic amino acid in humans. Leucine and β-hydroxy β-methylbutyric acid, a minor leucine metabolite, exhibit pharmacological activity in humans and have been demonstrated to promote protein biosynthesis via the phosphorylation of the mechanistic target of rapamycin (mTOR).

Chemical Structure

L-Leucine-1-13C

CAS#74292-94-7

Theoretical Analysis

MedKoo Cat#: 596455

Name: L-Leucine-1-13C

CAS#: 74292-94-7

Chemical Formula: C513CH13NO2

Exact Mass: 132.0980

Molecular Weight: 132.17

Elemental Analysis: C, 55.28; H, 9.91; N, 10.60; O, 24.21

Price and Availability

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1g	USD 585.00	Back order

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Synonym

L-Leucine-1-13C; L-(1-13C)Leucine; (1-13C)Leucine;

IUPAC/Chemical Name

L-leucine-1-13C

InChi Key

ROHFNLRQFUQHCH-SANWUMGISA-N

InChi Code

InChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1/i6+1

SMILES Code

N[C@@H](CC(C)C)[13C](O)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

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Product Data

Product Data

Instruction

View handling instruction

Biological target:

L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway.

In vitro activity:

On the other hand, Leu (L-leucine) treatment sharply decreased the β-cell number, as measured by insulin immunostaining (Fig. 2B and C for quantification). Such data indicate that l-Leu treatment represses β-cell development. Reference: Diabetes. 2012 Feb;61(2):409-17. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266409/

In vivo activity:

This study has explored the hypothesis that reduced ribosome function contributes to RBS in zebrafish models and human cells. To study this cohesinopathy in a whole animal model this study used ESCO2-mutant and morphant zebrafish embryos, which have developmental defects mimicking RBS. Stimulation of the TOR pathway with L-leucine rescued many developmental defects of ESCO2-mutant embryos. Reference: PLoS Genet. 2013;9(10):e1003857. https://pubmed.ncbi.nlm.nih.gov/24098154/

	Solvent	mg/mL	mM
Solubility
	Water	5.0	37.83

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 132.17 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Wu H, Dridi S, Huang Y, Baum JI. Leucine decreases intramyocellular lipid deposition in an mTORC1-independent manner in palmitate-treated C2C12 myotubes. Am J Physiol Endocrinol Metab. 2020 Feb 1;318(2):E152-E163. doi: 10.1152/ajpendo.00241.2019. Epub 2019 Nov 26. PMID: 31770014. 2. Rachdi L, Aïello V, Duvillié B, Scharfmann R. L-leucine alters pancreatic β-cell differentiation and function via the mTor signaling pathway. Diabetes. 2012 Feb;61(2):409-17. doi: 10.2337/db11-0765. Epub 2011 Dec 30. PMID: 22210321; PMCID: PMC3266409. 3. Wang X, Wang X, Xie F, Sun Z, Guo B, Li F, Wang S, Wang Y, Tian Y, Zhao Y, Qian L. Leucine mediates cognitive dysfunction in early life stress-induced mental disorders by activating autophagy. Front Cell Neurosci. 2023 Jan 4;16:1060712. doi: 10.3389/fncel.2022.1060712. PMID: 36687518; PMCID: PMC9846360. 4. Xu B, Lee KK, Zhang L, Gerton JL. Stimulation of mTORC1 with L-leucine rescues defects associated with Roberts syndrome. PLoS Genet. 2013;9(10):e1003857. doi: 10.1371/journal.pgen.1003857. Epub 2013 Oct 3. PMID: 24098154; PMCID: PMC3789817.

In vitro protocol:

In vivo protocol:

1. Wang X, Wang X, Xie F, Sun Z, Guo B, Li F, Wang S, Wang Y, Tian Y, Zhao Y, Qian L. Leucine mediates cognitive dysfunction in early life stress-induced mental disorders by activating autophagy. Front Cell Neurosci. 2023 Jan 4;16:1060712. doi: 10.3389/fncel.2022.1060712. PMID: 36687518; PMCID: PMC9846360. 2. Xu B, Lee KK, Zhang L, Gerton JL. Stimulation of mTORC1 with L-leucine rescues defects associated with Roberts syndrome. PLoS Genet. 2013;9(10):e1003857. doi: 10.1371/journal.pgen.1003857. Epub 2013 Oct 3. PMID: 24098154; PMCID: PMC3789817.

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