Methionine | CAS#63-68-3 | L-amino acid

MedKoo Cat#: 596412 | Name: Methionine

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Methionine is a sulfur-containing essential L-amino acid that is important in many body functions.

Chemical Structure

Methionine

CAS#63-68-3

Theoretical Analysis

MedKoo Cat#: 596412

Name: Methionine

CAS#: 63-68-3

Chemical Formula: C5H11NO2S

Exact Mass: 149.0510

Molecular Weight: 149.21

Elemental Analysis: C, 40.25; H, 7.43; N, 9.39; O, 21.45; S, 21.49

Price and Availability

Size	Price	Availability	Quantity
25g	USD 250.00	2 weeks
100g	USD 485.00	2 weeks

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Related CAS #

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Synonym

Methionine; MET; L-Methionine; NSC 22946; NSC-22946; NSC22946;

IUPAC/Chemical Name

L-methionine

InChi Key

FFEARJCKVFRZRR-BYPYZUCNSA-N

InChi Code

InChI=1S/C5H11NO2S/c1-9-3-2-4(6)5(7)8/h4H,2-3,6H2,1H3,(H,7,8)/t4-/m0/s1

SMILES Code

N[C@@H](CCSC)C(O)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Methionine is a sulfur-containing essential L-amino acid that is important in many body functions.

In vitro activity:

ARID1B knockdown promoted milk fat and protein synthesis in and cell proliferation of HC11 cells and increased mTOR mRNA expression and protein phosphorylation, whereas ARID1B gene activation had the opposite effects. ARID1B gene activation totally blocked Met's (methionine) stimulation on mTOR mRNA expression. ARID1B bound to one region of the mTOR promoter, and Met reduced the binding of ARID1B on this promoter. LY294002 blocked Met-induced reduction of ARID1B mRNA and protein level. Reference: J Nutr Biochem. 2023 Jan 18;114:109274. https://pubmed.ncbi.nlm.nih.gov/36681308/

In vivo activity:

This study found that PrL DBS and MET (L-methionine) (MET-PrL DBS) co-administration resulted in hippocampal-dependent spatial memory enhancements in aged animals. Molecular data suggested MET-PrL DBS induced DNA methyltransferase DNMT3a-dependent methylation, robust synergistic upregulation of neuroplasticity-related genes, and simultaneous inhibition of the memory-suppressing gene calcineurin in the hippocampus. Reference: Aging Dis. 2023 Feb 1;14(1):112-135. https://pubmed.ncbi.nlm.nih.gov/36818556/

	Solvent	mg/mL	mM	comments
Solubility
	Water	22.8	153.04

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 149.21 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Lin G, Qi H, Guo X, Wang W, Zhang M, Gao X. ARID1B blocks methionine-stimulated mTOR activation to inhibit milk fat and protein synthesis in and proliferation of mouse mammary epithelial cells. J Nutr Biochem. 2023 Jan 18;114:109274. doi: 10.1016/j.jnutbio.2023.109274. Epub ahead of print. PMID: 36681308. 2. Yokogami K, Kikuchi T, Watanabe T, Nakatake Y, Yamashita S, Mizuguchi A, Takeshima H. Methionine regulates self-renewal, pluripotency, and cell death of GIC through cholesterol-rRNA axis. BMC Cancer. 2022 Dec 23;22(1):1351. doi: 10.1186/s12885-022-10280-5. PMID: 36564758; PMCID: PMC9789638. 3. Poon CH, Liu Y, Pak S, Zhao RC, Aquili L, Tipoe GL, Leung GK, Chan YS, Yang S, Fung ML, Wu EX, Lim LW. Prelimbic Cortical Stimulation with L-methionine Enhances Cognition through Hippocampal DNA Methylation and Neuroplasticity Mechanisms. Aging Dis. 2023 Feb 1;14(1):112-135. doi: 10.14336/AD.2022.0706. PMID: 36818556; PMCID: PMC9937711. 4. Wang Y, Yang L, Zhou H, Zhang K, Zhao M. Identification of miRNA-mediated gene regulatory networks in L-methionine exposure counteracts cocaine-conditioned place preference in mice. Front Genet. 2023 Jan 19;13:1076156. doi: 10.3389/fgene.2022.1076156. PMID: 36744178; PMCID: PMC9893020.

In vitro protocol:

In vivo protocol:

1. Poon CH, Liu Y, Pak S, Zhao RC, Aquili L, Tipoe GL, Leung GK, Chan YS, Yang S, Fung ML, Wu EX, Lim LW. Prelimbic Cortical Stimulation with L-methionine Enhances Cognition through Hippocampal DNA Methylation and Neuroplasticity Mechanisms. Aging Dis. 2023 Feb 1;14(1):112-135. doi: 10.14336/AD.2022.0706. PMID: 36818556; PMCID: PMC9937711. 2. Wang Y, Yang L, Zhou H, Zhang K, Zhao M. Identification of miRNA-mediated gene regulatory networks in L-methionine exposure counteracts cocaine-conditioned place preference in mice. Front Genet. 2023 Jan 19;13:1076156. doi: 10.3389/fgene.2022.1076156. PMID: 36744178; PMCID: PMC9893020.

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