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MedKoo Cat#: 596130 | Name: Fast Green FCF
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Fast Green FCF is a sea green triarylmethane food dye. Fast Green FCF is used as a quantitative stain for histones at alkaline pH after acid extraction of DNA. It is also used as a protein stain in electrophoresis. Its absorption maximum is at 625 nm.

Chemical Structure

Fast Green FCF
Fast Green FCF
CAS#2353-45-9 (sodium)

Theoretical Analysis

MedKoo Cat#: 596130

Name: Fast Green FCF

CAS#: 2353-45-9 (sodium)

Chemical Formula: C37H36N2Na2O10S3

Exact Mass: 0.0000

Molecular Weight: 810.85

Elemental Analysis: C, 54.81; H, 4.48; N, 3.45; Na, 5.67; O, 19.73; S, 11.86

Price and Availability

Size Price Availability Quantity
1g USD 250.00 2 Weeks
5g USD 450.00 2 Weeks
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Related CAS #
2353-45-9 (sodium) 25738-40-3 (free acid)
Synonym
Fast Green FCF; Food green 3; FD&C Green No. 3; Solid Green FCF; C.I. Food Green 3; C.I. 42053; Zelen Stala FCF; Japan Green 3; NSC 379443; NSC379443; NSC-379443;
IUPAC/Chemical Name
sodium 2-((Z)-(4-(ethyl(3-sulfonatobenzyl)amino)phenyl)((Z)-4-(ethyl(3-sulfonatobenzyl)iminio)cyclohexa-2,5-dien-1-ylidene)methyl)-5-hydroxybenzenesulfonate
InChi Key
RZSYLLSAWYUBPE-UHFFFAOYSA-L
InChi Code
InChI=1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
SMILES Code
O=S(C1=CC(O)=CC=C1/C(C2=CC=C(N(CC)CC3=CC=CC(S(=O)([O-])=O)=C3)C=C2)=C4C=C/C(C=C\4)=[N+](CC)\CC5=CC=CC(S(=O)([O-])=O)=C5)([O-])=O.[Na+].[Na+]
Appearance
Solid powder
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Fast Green FCF is a sea green triarylmethane food dye.
In vitro activity:
A safe food additive, fast green FCF, is capable of inhibiting α-synuclein fibrillogenesis and reducing the related cytotoxicity. Thioflavin T fluorescence assays demonstrated that fast green FCF could inhibit the fibrillogenesis α-synuclein. Reference: Food Funct. 2021 Jun 21;12(12):5465-5477. https://pubmed.ncbi.nlm.nih.gov/33997868/
In vivo activity:
Moreover, FGF (fast green FCF) showed a protective effect against cognitive impairment in Aβ-treated mice. Reference: Int J Biol Macromol. 2021 Feb 15;170:33-41. https://pubmed.ncbi.nlm.nih.gov/33352157/
Solvent mg/mL mM
Solubility
DMSO 32.5 40.08
DMF 0.5 0.62
Ethanol 0.5 0.62
PBS (pH 7.2) 10.0 12.33
Water 14.0 17.27
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 810.85 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Wang F, Wang Y, Jiang L, Wang W, Sang J, Wang X, Lu F, Liu F. The food additive fast green FCF inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity. Food Funct. 2021 Jun 21;12(12):5465-5477. doi: 10.1039/d0fo03301d. PMID: 33997868. 2. Liu F, Wang W, Xuan Z, Jiang L, Chen B, Dong Q, Zhao F, Cui W, Li L, Lu F. Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice. Int J Biol Macromol. 2021 Feb 15;170:33-41. doi: 10.1016/j.ijbiomac.2020.12.115. Epub 2020 Dec 19. PMID: 33352157. 3. Yang J, Liu R, Lu F, Xu F, Zheng J, Li Z, Cui W, Wang C, Zhang J, Xu S, Zhou W, Wang Q, Chen J, Chen X. Fast Green FCF Attenuates Lipopolysaccharide-Induced Depressive-Like Behavior and Downregulates TLR4/Myd88/NF-κB Signal Pathway in the Mouse Hippocampus. Front Pharmacol. 2019 May 8;10:501. doi: 10.3389/fphar.2019.00501. PMID: 31139084; PMCID: PMC6519320.
In vitro protocol:
1. Wang F, Wang Y, Jiang L, Wang W, Sang J, Wang X, Lu F, Liu F. The food additive fast green FCF inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity. Food Funct. 2021 Jun 21;12(12):5465-5477. doi: 10.1039/d0fo03301d. PMID: 33997868.
In vivo protocol:
1. Liu F, Wang W, Xuan Z, Jiang L, Chen B, Dong Q, Zhao F, Cui W, Li L, Lu F. Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice. Int J Biol Macromol. 2021 Feb 15;170:33-41. doi: 10.1016/j.ijbiomac.2020.12.115. Epub 2020 Dec 19. PMID: 33352157. 2. Yang J, Liu R, Lu F, Xu F, Zheng J, Li Z, Cui W, Wang C, Zhang J, Xu S, Zhou W, Wang Q, Chen J, Chen X. Fast Green FCF Attenuates Lipopolysaccharide-Induced Depressive-Like Behavior and Downregulates TLR4/Myd88/NF-κB Signal Pathway in the Mouse Hippocampus. Front Pharmacol. 2019 May 8;10:501. doi: 10.3389/fphar.2019.00501. PMID: 31139084; PMCID: PMC6519320.
1: Alzahrani E. Chitosan Membrane Embedded With ZnO/CuO Nanocomposites for the Photodegradation of Fast Green Dye Under Artificial and Solar Irradiation. Anal Chem Insights. 2018 Mar 21;13:1177390118763361. doi: 10.1177/1177390118763361. eCollection 2018. PubMed PMID: 29593375; PubMed Central PMCID: PMC5865519. 2: Abdi S, Nasiri M. Removal of Fast Green FCF dye from aqueous solutions using Flower Gel as a low-cost adsorbent. Water Sci Technol. 2018 Mar;77(5):1213-1221. doi: 10.2166/wst.2017.633. PubMed PMID: 29528309; PubMed Central PMCID: wst_2017_633. 3: Pankaj SK, Wan Z, Colonna W, Keener KM. Degradation kinetics of organic dyes in water by high voltage atmospheric air and modified air cold plasma. Water Sci Technol. 2017 Jul;76(3-4):567-574. doi: 10.2166/wst.2017.169. PubMed PMID: 28759439; PubMed Central PMCID: wst_2017_169. 4: Pooralhossini J, Ghaedi M, Zanjanchi MA, Asfaram A. Ultrasonically assisted removal of Congo Red, Phloxine B and Fast green FCF in ternary mixture using novel nanocomposite following their simultaneous analysis by derivative spectrophotometry. Ultrason Sonochem. 2017 Jul;37:452-463. doi: 10.1016/j.ultsonch.2017.01.038. Epub 2017 Jan 30. PubMed PMID: 28427656. 5: Giers MB, Munter BT, Eyster KJ, Ide GD, Newcomb AGUS, Lehrman JN, Belykh E, Byvaltsev VA, Kelly BP, Preul MC, Theodore N. Biomechanical and Endplate Effects on Nutrient Transport in the Intervertebral Disc. World Neurosurg. 2017 Mar;99:395-402. doi: 10.1016/j.wneu.2016.12.041. Epub 2016 Dec 21. PubMed PMID: 28012886. 6: Tikhomirova TI, Ramazanova GR, Apyari VV. A hybrid sorption - Spectrometric method for determination of synthetic anionic dyes in foodstuffs. Food Chem. 2017 Apr 15;221:351-355. doi: 10.1016/j.foodchem.2016.10.042. Epub 2016 Oct 11. PubMed PMID: 27979213. 7: Deniz F, Kepekci RA. Biosorption of Food Green 3 by a novel green generation composite biosorbent from aqueous environment. Int J Phytoremediation. 2017 Jun 3;19(6):579-586. doi: 10.1080/15226514.2016.1267707. PubMed PMID: 27936896. 8: How SC, Yang SM, Hsin A, Tseng CP, Hsueh SS, Lin MS, Chen RP, Chou WL, Wang SS. Examining the inhibitory potency of food additive fast green FCF against amyloid fibrillogenesis under acidic conditions. Food Funct. 2016 Dec 7;7(12):4898-4907. PubMed PMID: 27824368. 9: Stachová I, Lhotská I, Solich P, Šatínský D. Determination of green, blue and yellow artificial food colorants and their abuse in herb-coloured green Easter beers on tap. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2016 Jul;33(7):1139-46. doi: 10.1080/19440049.2016.1200198. Epub 2016 Jul 1. PubMed PMID: 27295128. 10: Lawrence PG, Patil PS, Leipzig ND, Lapitsky Y. Ionically Cross-Linked Polymer Networks for the Multiple-Month Release of Small Molecules. ACS Appl Mater Interfaces. 2016 Feb;8(7):4323-35. doi: 10.1021/acsami.5b10070. Epub 2016 Feb 11. PubMed PMID: 26811936; PubMed Central PMCID: PMC4768684. 11: Li T, Cheng Z, Cao L, Jiang X. Comparison of interactions between three food colorants and BSA. Food Chem. 2016 Mar 1;194:740-8. doi: 10.1016/j.foodchem.2015.08.067. Epub 2015 Aug 20. PubMed PMID: 26471614. 12: Ding DF, Pang J, Song Y, Du GQ, Cao YL, Zhan HS, Zheng YX. [Etablishment of cartilage degeneration model by IL-1 beta in vitro]. Zhongguo Gu Shang. 2015 Jul;28(7):648-53. Chinese. PubMed PMID: 26399109. 13: Tsai CF, Kuo CH, Shih DY. Determination of 20 synthetic dyes in chili powders and syrup-preserved fruits by liquid chromatography/tandem mass spectrometry. J Food Drug Anal. 2015 Sep;23(3):453-462. doi: 10.1016/j.jfda.2014.09.003. Epub 2015 Jan 1. PubMed PMID: 28911703. 14: Du G, Song Y, Wei L, Li L, Wang X, Xu Q, Zhan H, Cao Y, Zheng Y, Ding D. Osthole Inhibits Proliferation and Induces Catabolism in Rat Chondrocytes and Cartilage Tissue. Cell Physiol Biochem. 2015;36(6):2480-93. doi: 10.1159/000430208. Epub 2015 Aug 10. PubMed PMID: 26279449. 15: Huang X, Huang BS, Wang LF, Liu YW, Zou PC, Li WS, Yu K. [Anatomical structures of Coleus forskohlii transplanted in Tongcheng]. Zhong Yao Cai. 2014 Feb;37(2):230-2. Chinese. PubMed PMID: 25095342. 16: de Silva UK, Weik BE, Lapitsky Y. Simple preparation of polyelectrolyte complex beads for the long-term release of small molecules. Langmuir. 2014 Jul 29;30(29):8915-22. doi: 10.1021/la501771k. Epub 2014 Jul 16. PubMed PMID: 24992631. 17: Ha MS, Ha SD, Choi SH, Bae DH. Exposure assessment of synthetic colours approved in Korea. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(4):643-53. doi: 10.1080/19440049.2013.768358. Epub 2013 Mar 25. PubMed PMID: 23521141. 18: Boss D, Kühn J, Jourdain P, Depeursinge C, Magistretti PJ, Marquet P. Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy. J Biomed Opt. 2013 Mar;18(3):036007. doi: 10.1117/1.JBO.18.3.036007. PubMed PMID: 23487181. 19: Wong HE, Qi W, Choi HM, Fernandez EJ, Kwon I. A safe, blood-brain barrier permeable triphenylmethane dye inhibits amyloid-β neurotoxicity by generating nontoxic aggregates. ACS Chem Neurosci. 2011 Nov 16;2(11):645-57. doi: 10.1021/cn200056g. Epub 2011 Sep 6. PubMed PMID: 22860159; PubMed Central PMCID: PMC3369715. 20: Chitambar M, Wang Z, Liu Y, Rockett A, Maldonado S. Dye-sensitized photocathodes: efficient light-stimulated hole injection into p-GaP under depletion conditions. J Am Chem Soc. 2012 Jun 27;134(25):10670-81. doi: 10.1021/ja304019n. Epub 2012 Jun 18. PubMed PMID: 22734693.