WST-3 is a water-soluble tetrazolium dye commonly used in cell viability and cytotoxicity assays. In the presence of an electron mediator, such as 1-methoxy-5-methylphenazinium, WST-3 is reduced by NADH to form a water-soluble formazan product, which exhibits a dark red color. This color change can be quantitatively measured by its absorbance, providing an indicator of cellular metabolic activity.
MedKoo Cat#: 562733
Name: WST-3
CAS#: 515111-36-1
Chemical Formula: C19H10IN6NaO10S2
Exact Mass: 0.0000
Molecular Weight: 696.33
Elemental Analysis: C, 32.77; H, 1.45; I, 18.22; N, 12.07; Na, 3.30; O, 22.98; S, 9.21
The following data is based on the product molecular weight 696.33 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
