Hoffman's violet is specific stains for animal chromosomes. Iodinated alcohol after staining can be substituted with various acids, both organic as well as inorganic, all of which act as trapping agent preventing leaching of the dye that binds with the chromosomal DNA. RNA is not involved by this process of staining, since treatment of stained sections with cold phosphoric acid at 5 degrees C for 20--25 min and then stained also reveals perfect colouration of the chromosomes without any cytoplasmic staining.
MedKoo Cat#: 581233
Name: Hofman's Violet
CAS#: 8004-86-2
Chemical Formula: C26H32ClN3
Exact Mass: 421.2285
Molecular Weight: 422.01
Elemental Analysis: C, 74.00; H, 7.64; Cl, 8.40; N, 9.96
The following data is based on the product molecular weight 422.01 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
