MedKoo Cat#: 571641 | Name: Biotin Hydrazide
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Biotin Hydrazide, or Biotin-Hz, is a carbohydrate reactive biotinylation reagent. Biotin Hydrazide is a great choice for labeling mouse monoclonal antibodies, lectins, sugars, unpaired cytosine residues in nucleic acids, and glycoprotiens.

Chemical Structure

Biotin Hydrazide
Biotin Hydrazide
CAS#66640-86-6

Theoretical Analysis

MedKoo Cat#: 571641

Name: Biotin Hydrazide

CAS#: 66640-86-6

Chemical Formula: C10H18N4O2S

Exact Mass: 258.1150

Molecular Weight: 258.34

Elemental Analysis: C, 46.49; H, 7.02; N, 21.69; O, 12.39; S, 12.41

Price and Availability

Size Price Availability Quantity
250mg USD 350.00 2 Weeks
1g USD 650.00 2 Weeks
5g USD 1,250.00 2 Weeks
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
Biotin Hydrazide, (+)-Biotin Hydrazide, Hydrazide biotin, Biotine hydrazide, Biotin-hydrazide, Biotin-Hz
IUPAC/Chemical Name
5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanehydrazide
InChi Key
KOZWHQPRAOJMBN-ZKWXMUAHSA-N
InChi Code
InChI=1S/C10H18N4O2S/c11-14-8(15)4-2-1-3-7-9-6(5-17-7)12-10(16)13-9/h6-7,9H,1-5,11H2,(H,14,15)(H2,12,13,16)/t6-,7-,9-/m0/s1
SMILES Code
O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NN)=O
Appearance
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Typically, Biotin Hydrazide is used for coupling glycoproteins through the carbohydrate by hydrazone formation. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin or Immobilized Streptavidin.

Preparing Stock Solutions

The following data is based on the product molecular weight 258.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Hensley K. Detection of Protein Carbonyls by Means of Biotin Hydrazide-Streptavidin Affinity Methods. Methods Mol Biol. 2015;1314:95-100. doi: 10.1007/978-1-4939-2718-0_11. PubMed PMID: 26139258. 2: Wu J, Luo X, Jing S, Yan LJ. Two-dimensional gel electrophoretic detection of protein carbonyls derivatized with biotin-hydrazide. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Apr 15;1019:128-31. doi: 10.1016/j.jchromb.2015.11.003. Epub 2015 Nov 7. PubMed PMID: 26590475; PubMed Central PMCID: PMC4829449. 3: Massó F, Varela E, Páez A, Zenteno E, Montaño LF. Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling. Glycoconj J. 1998 Sep;15(9):843-6. PubMed PMID: 10052587. 4: Reisfeld A, Rothenberg JM, Bayer EA, Wilchek M. Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. Biochem Biophys Res Commun. 1987 Jan 30;142(2):519-26. PubMed PMID: 3101692. 5: Hensley K. Detection of protein carbonyls by means of biotin hydrazide-streptavidin affinity methods. Methods Mol Biol. 2009;536:457-62. doi: 10.1007/978-1-59745-542-8_46. PubMed PMID: 19378083. 6: Rubio-González C, Fernández-Abedul MT, Costa-García A. Comparative electrochemical behaviour of biotin hydrazide and photobiotin. Importance in the development of biosensors. Biosens Bioelectron. 1999 Dec;14(8-9):729-35. PubMed PMID: 10641292. 7: Gómez-Sánchez CE, León LM, Gómez-Sánchez EP. Biotin-hydrazide derivatives for the development of steroid enzyme-linked immunoassays. J Steroid Biochem Mol Biol. 1992 Nov;43(6):523-7. PubMed PMID: 1419887.