MedKoo Cat#: 571613 | Name: Sulfo-LC-SPDP Crosslinker
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Sulfo-LC-SPDP Crosslinker, or Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, is a cleavable, water soluble, amino and thiol (sulfhydryl) reactive heterobifunctional protein crosslinker. The "long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 angstrom spacer arm, and is the water soluble analog of LC-SPDP crosslinker.

Chemical Structure

Sulfo-LC-SPDP Crosslinker
Sulfo-LC-SPDP Crosslinker
CAS#169751-10-4 (sodium)

Theoretical Analysis

MedKoo Cat#: 571613

Name: Sulfo-LC-SPDP Crosslinker

CAS#: 169751-10-4 (sodium)

Chemical Formula: C18H22N3NaO8S3

Exact Mass:

Molecular Weight: 527.56

Elemental Analysis: C, 40.98; H, 4.20; N, 7.97; Na, 4.36; O, 24.26; S, 18.23

Price and Availability

Size Price Availability Quantity
100mg USD 750.00 2 Weeks
1g USD 4,850.00 2 Weeks
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Related CAS #
169751-10-4 (sodium) 150244-18-1 (acid)
Synonym
Sulfo-LC-SPDP Crosslinker, Sulfo-LC-SPDP Cross linker, Sulfo-LC-SPDP Cross-linker; Sulfo-AC5-SPDP; Sulfo-LC-SPDP;
IUPAC/Chemical Name
3-Pyrrolidinesulfonic acid, 2,5-dioxo-1-[[1-oxo-6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]oxy]-, monosodium salt
InChi Key
ZAPNXDUFCQIHFS-UHFFFAOYSA-M
InChi Code
InChI=1S/C18H23N3O8S3.Na/c22-14(8-11-30-31-15-6-3-5-10-20-15)19-9-4-1-2-7-17(24)29-21-16(23)12-13(18(21)25)32(26,27)28;/h3,5-6,10,13H,1-2,4,7-9,11-12H2,(H,19,22)(H,26,27,28);/q;+1/p-1
SMILES Code
O=S(C(C1)C(N(OC(CCCCCNC(CCSSC2=NC=CC=C2)=O)=O)C1=O)=O)([O-])=O.[Na+]
Appearance
Solid powder
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Sulfo-LC-SPDP crosslinker's reactive groups include N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains; while on the other end of the Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond. The disulfide bond can be reduced with TCEP-HCl or DTT to reverse the crosslinked proteins.

Preparing Stock Solutions

The following data is based on the product molecular weight 527.56 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Weston LA, Bauer KM, Skube SB, Hummon AB. Selective, bead-based global peptide capture using a bifunctional cross-linker. Anal Chem. 2013 Nov 19;85(22):10675-9. doi: 10.1021/ac401825m. Epub 2013 Oct 28. PubMed PMID: 24117407; PubMed Central PMCID: PMC4118834.