NP-313 | CAS#5397-78-4 | antithrombotic agent

MedKoo Cat#: 562405 | Name: NP-313

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

NP-313 is an antithrombotic agent with dual inhibition of thromboxane A2 synthesis and calcium entry.

Chemical Structure

NP-313

CAS#5397-78-4

Theoretical Analysis

MedKoo Cat#: 562405

Name: NP-313

CAS#: 5397-78-4

Chemical Formula: C12H8ClNO3

Exact Mass: 249.0193

Molecular Weight: 249.65

Elemental Analysis: C, 57.73; H, 3.23; Cl, 14.20; N, 5.61; O, 19.23

Price and Availability

Size	Price	Availability	Quantity
5mg	USD 260.00

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Synonym

NP-313; NP 313; NP313; NSC-4264; NSC 4264; NSC4264;

IUPAC/Chemical Name

2-Acetylamino-3-chloro-1,4-naphthoquinone

InChi Key

OMXMYDUYAUOFRX-UHFFFAOYSA-N

InChi Code

InChI=1S/C12H8ClNO3/c1-6(15)14-10-9(13)11(16)7-4-2-3-5-8(7)12(10)17/h2-5H,1H3,(H,14,15)

SMILES Code

O=C(C1=C2C=CC=C1)C(Cl)=C(NC(C)=O)C2=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

NP-313 is a potent antithrombotic agent that inhibits platelet aggregation and activation.

In vitro activity:

NP-313 concentration-dependently inhibited human platelet aggregation induced by collagen, arachidonic acid, thapsigargin, thrombin and A23187. NP-313 also inhibited P-selectin expression, thromboxane B(2) formation and [Ca(2+) ](i) elevation in platelets stimulated by thrombin and collagen. NP-313 at 10 µM inhibited cyclooxygenase, thromboxane A(2) synthase, and protein kinase Cα, whereas it did not affect phospholipase A(2) or phospholipase C activity. Reference: Br J Pharmacol. 2011 Apr;162(8):1871-83. https://pubmed.ncbi.nlm.nih.gov/21232029/

In vivo activity:

This study evaluated the ex vivo antiplatelet action of NP-313 on PRP. As shown in Figure 9C, upon i.v. administration of NP-313 at 4, 8 and 16 µg·g−1 for mice, NP-313 inhibited ex vivo platelet aggregation of PRP caused by collagen (10 µg·mL−1) 5 min after drug administration. Reference: Br J Pharmacol. 2011 Apr;162(8):1871-83. https://pubmed.ncbi.nlm.nih.gov/21232029/

Preparing Stock Solutions

The following data is based on the product molecular weight 249.65 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

Kuo HL, Lien JC, Chang CH, Chung CH, Kuo SC, Hsu CC, Peng HC, Huang TF. NP-313, 2-acetylamino-3-chloro-1,4-naphthoquinone, a novel antithrombotic agent with dual inhibition of thromboxane A(2) synthesis and calcium entry. Br J Pharmacol. 2011 Apr;162(8):1871-83. doi: 10.1111/j.1476-5381.2011.01200.x. PMID: 21232029; PMCID: PMC3081128.

In vitro protocol:

In vivo protocol:

1: Werner WJ, Allen KD, Hu K, Helms GL, Chen BS, Wang SC. In vitro phosphinate methylation by PhpK from Kitasatospora phosalacinea. Biochemistry. 2011 Oct 25;50(42):8986-8. doi: 10.1021/bi201220r. Epub 2011 Sep 28. PubMed PMID: 21950770; PubMed Central PMCID: PMC3214642. 2: Kuo HL, Lien JC, Chang CH, Chung CH, Kuo SC, Hsu CC, Peng HC, Huang TF. NP-313, 2-acetylamino-3-chloro-1,4-naphthoquinone, a novel antithrombotic agent with dual inhibition of thromboxane A(2) synthesis and calcium entry. Br J Pharmacol. 2011 Apr;162(8):1871-83. doi: 10.1111/j.1476-5381.2011.01200.x. PubMed PMID: 21232029; PubMed Central PMCID: PMC3081128.