MedKoo Cat#: 561720 | Name: EthD-1
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

EthD-1 is a cell-impermeant viability indicator. It acts as a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~528/617).

Chemical Structure

EthD-1
EthD-1
CAS#61926-22-5 (HCl)

Theoretical Analysis

MedKoo Cat#: 561720

Name: EthD-1

CAS#: 61926-22-5 (HCl)

Chemical Formula: C46H50Cl4N8

Exact Mass: 0.0000

Molecular Weight: 856.76

Elemental Analysis: C, 64.49; H, 5.88; Cl, 16.55; N, 13.08

Price and Availability

Size Price Availability Quantity
1mg USD 750.00 2 Weeks
5mg USD 2,650.00 2 Weeks
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Related CAS #
61926-22-5 (HCl) 68687-01-4 (cation)
Synonym
EthD-1; EthD 1; EthD1; Ethidium homodimer; Ethidium homodimer 1
IUPAC/Chemical Name
5,5'-((ethane-1,2-diylbis(azanediyl))bis(propane-3,1-diyl))bis(3,8-diamino-6-phenylphenanthridin-5-ium) chloride dihydrochloride
InChi Key
ZKAJUKABUUJRLB-UHFFFAOYSA-N
InChi Code
InChI=1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
SMILES Code
NC1=CC2=C(C3=CC=CC=C3)[N+](CCCNCCNCCC[N+]4=C(C=C(N)C=C5)C5=C(C=CC(N)=C6)C6=C4C7=CC=CC=C7)=C(C=C(N)C=C8)C8=C2C=C1.[Cl-].[Cl-].Cl.Cl
Appearance
Solid powder
Purity
>90% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 856.76 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Gillan L, Evans G, Maxwell WM. Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology. 2005 Jan 15;63(2):445-57. Review. PubMed PMID: 15626410. 2: Kaplan LD. The analysis of articular cartilage after thermal exposure: "Is red really dead?". Arthroscopy. 2003 Mar;19(3):310-3. Review. PubMed PMID: 12627157. 3: Chrambach A, Yarmola E, Zakharov SF, Garner MM. Commercial automated gel electrophoresis apparatus: application to DNA, band dispersion, nonlinear Ferguson curves, and isolation. Electrophoresis. 1995 May;16(5):713-8. Review. PubMed PMID: 7588549.