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MedKoo Cat#: 561711 | Name: Robenidine HCl
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Robenidine HCl is an antibiotic used for the control of coccidiosis, a debilitating protozoal infection in poultry.

Chemical Structure

Robenidine HCl
Robenidine HCl
CAS#25875-50-7 (HCl)

Theoretical Analysis

MedKoo Cat#: 561711

Name: Robenidine HCl

CAS#: 25875-50-7 (HCl)

Chemical Formula: C15H14Cl3N5

Exact Mass:

Molecular Weight: 370.66

Elemental Analysis: C, 48.61; H, 3.81; Cl, 28.69; N, 18.89

Price and Availability

Size Price Availability Quantity
10mg USD 90.00 2 Weeks
100mg USD 250.00 2 Weeks
200mg USD 450.00 2 Weeks
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Related CAS #
25875-51-8 (free base) 25875-50-7 (HCl)
Synonym
Robenidine hydrochloride; Robenidine HCl; Cycostat; Chimcoccide.
IUPAC/Chemical Name
1,3-Bis[(4-chlorobenzylidene)amino]guanidine monohydrochloride
InChi Key
LTWIBTYLSRDGHP-HCURTGQUSA-N
InChi Code
InChI=1S/C15H13Cl2N5.ClH/c16-13-5-1-11(2-6-13)9-19-21-15(18)22-20-10-12-3-7-14(17)8-4-12;/h1-10H,(H3,18,21,22);1H/b19-9+,20-10+;
SMILES Code
N=C(N/N=C/C1=CC=C(Cl)C=C1)N/N=C/C2=CC=C(Cl)C=C2.[H]Cl
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Robenidine hydrochloride is a coccidiostat and antibiotic.
In vitro activity:
Robenidine displays a promising and broadly effective antifungal strategy. Physiological concentration of robenidine was able to significantly inhibit yeast cell growth, filamentation, and biofilm formation of Candida albicans. Robenidine-treated C. albicans cells were hypersensitive to diverse cell wall stressors. Analysis of the cell wall structure showed that the cell wall was severely damaged by robenidine Reference: Front Microbiol. 2020 Jun 3;11:996. https://pubmed.ncbi.nlm.nih.gov/32582050/
In vivo activity:
This study supports robenidine as a valid candidate for further development to treat multidrug-resistant bacterial infections. In a bioluminescent S. aureus sepsis challenge model, mice that received robenidine exhibited significantly reduced bacterial loads, longer survival times, and higher overall survival rates than the vehicle-only treated mice. Reference: Antibiotics (Basel). 2022 Jan 6;11(1):65. https://pubmed.ncbi.nlm.nih.gov/35052942/
Solvent mg/mL mM
Solubility
DMSO 74.0 199.64
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 370.66 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Mei Y, Jiang T, Zou Y, Wang Y, Zhou J, Li J, Liu L, Tan J, Wei L, Li J, Dai H, Peng Y, Zhang L, Lopez-Ribot JL, Shapiro RS, Chen C, Liu NN, Wang H. FDA Approved Drug Library Screening Identifies Robenidine as a Repositionable Antifungal. Front Microbiol. 2020 Jun 3;11:996. doi: 10.3389/fmicb.2020.00996. PMID: 32582050; PMCID: PMC7283467. 2. Khazandi M, Pi H, Chan WY, Ogunniyi AD, Sim JXF, Venter H, Garg S, Page SW, Hill PB, McCluskey A, Trott DJ. In vitro Antimicrobial Activity of Robenidine, Ethylenediaminetetraacetic Acid and Polymyxin B Nonapeptide Against Important Human and Veterinary Pathogens. Front Microbiol. 2019 Apr 25;10:837. doi: 10.3389/fmicb.2019.00837. PMID: 31105656; PMCID: PMC6494957. 3. Nguyen HT, Venter H, Woolford L, Young K, McCluskey A, Garg S, Page SW, Trott DJ, Ogunniyi AD. Impact of a Novel Anticoccidial Analogue on Systemic Staphylococcus aureus Infection in a Bioluminescent Mouse Model. Antibiotics (Basel). 2022 Jan 6;11(1):65. doi: 10.3390/antibiotics11010065. PMID: 35052942; PMCID: PMC8773087. 4. Yao JM, Zhang HB, Liu CS, Tao Y, Yin M. Inhibitory effects of 19 antiprotozoal drugs and antibiotics on Babesia microti infection in BALB/c mice. J Infect Dev Ctries. 2015 Sep 27;9(9):1004-10. doi: 10.3855/jidc.5500. PMID: 26409742.
In vitro protocol:
1. Mei Y, Jiang T, Zou Y, Wang Y, Zhou J, Li J, Liu L, Tan J, Wei L, Li J, Dai H, Peng Y, Zhang L, Lopez-Ribot JL, Shapiro RS, Chen C, Liu NN, Wang H. FDA Approved Drug Library Screening Identifies Robenidine as a Repositionable Antifungal. Front Microbiol. 2020 Jun 3;11:996. doi: 10.3389/fmicb.2020.00996. PMID: 32582050; PMCID: PMC7283467. 2. Khazandi M, Pi H, Chan WY, Ogunniyi AD, Sim JXF, Venter H, Garg S, Page SW, Hill PB, McCluskey A, Trott DJ. In vitro Antimicrobial Activity of Robenidine, Ethylenediaminetetraacetic Acid and Polymyxin B Nonapeptide Against Important Human and Veterinary Pathogens. Front Microbiol. 2019 Apr 25;10:837. doi: 10.3389/fmicb.2019.00837. PMID: 31105656; PMCID: PMC6494957.
In vivo protocol:
1. Nguyen HT, Venter H, Woolford L, Young K, McCluskey A, Garg S, Page SW, Trott DJ, Ogunniyi AD. Impact of a Novel Anticoccidial Analogue on Systemic Staphylococcus aureus Infection in a Bioluminescent Mouse Model. Antibiotics (Basel). 2022 Jan 6;11(1):65. doi: 10.3390/antibiotics11010065. PMID: 35052942; PMCID: PMC8773087. 2. Yao JM, Zhang HB, Liu CS, Tao Y, Yin M. Inhibitory effects of 19 antiprotozoal drugs and antibiotics on Babesia microti infection in BALB/c mice. J Infect Dev Ctries. 2015 Sep 27;9(9):1004-10. doi: 10.3855/jidc.5500. PMID: 26409742.
1: Yao JM, Zhang HB, Liu CS, Tao Y, Yin M. Inhibitory effects of 19 antiprotozoal drugs and antibiotics on Babesia microti infection in BALB/c mice. J Infect Dev Ctries. 2015 Sep 27;9(9):1004-10. doi: 10.3855/jidc.5500. PubMed PMID: 26409742. 2: Delahaut P, Pierret G, Ralet N, Dubois M, Gillard N. Multi-residue method for detecting coccidiostats at carry-over level in feed by HPLC-MS/MS. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 Jun;27(6):801-9. doi: 10.1080/19440040903552408. PubMed PMID: 20198524. 3: Norton CC, Wise DR. Anticoccidial drugs for preventive therapy in intensively reared pheasants. Vet Rec. 1981 Dec 19;109(25-26):554-6. PubMed PMID: 7336544. 4: Zagar JB, Ascione PP, Chrekian GP. Automated high-pressure liquid chromatographic assay of robenidine hydrochloride. J Assoc Off Anal Chem. 1975 Jul;58(4):822-7. PubMed PMID: 1150616. 5: Zulalian J, Champagne DA, Wayne RS, Blinn RC. Absorption, excretion, and metabolism of 1,3-bis(p-chlorobenzylideneamino)guanidine hydrochloride (Robenz robenidine hydrochloride) in the chicken. J Agric Food Chem. 1975 Jul-Aug;23(4):724-30. PubMed PMID: 1141522. 6: Zulalian J, Gatterdam PE. Absorption, excretion, and metabolism of robenz, robenidine hydrochloride (1,3-bis(p-chlorobenzylideneamino)guanidine hydrochloride), in the rat. J Agric Food Chem. 1973 Sep-Oct;21(5):794-7. PubMed PMID: 4733370.