Riboflavin Sodium Phosphate | CAS#130-40-5 | coenzyme

MedKoo Cat#: 561591 | Name: Riboflavin Sodium Phosphate

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Riboflavin Sodium Phosphate is a coenzyme for a number of oxidative enzymes including NADH dehydrogenase.

Chemical Structure

Riboflavin Sodium Phosphate

CAS#130-40-5

Theoretical Analysis

MedKoo Cat#: 561591

Name: Riboflavin Sodium Phosphate

CAS#: 130-40-5

Chemical Formula: C17H20N4NaO9P

Exact Mass: 0.0000

Molecular Weight: 478.32

Elemental Analysis: C, 42.69; H, 4.21; N, 11.71; Na, 4.81; O, 30.10; P, 6.48

Price and Availability

Size	Price	Availability	Quantity
5g	USD 250.00	2 weeks

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Synonym

Riboflavin Sodium Phosphate; Hyryl; Flavin mononucleotide; Riboflavin 5-phosphate sodium;

IUPAC/Chemical Name

Sodium [(2R,3S,4S)-5-(7,8-dimethyl-2,4-dioxobenzo[g]pteridin-10-yl)-2,3,4-trihydroxypentyl] hydrogen phosphate

InChi Key

OHSHFZJLPYLRIP-BMZHGHOISA-M

InChi Code

InChI=1S/C17H21N4O9P.Na/c1-7-3-9-10(4-8(7)2)21(15-13(18-9)16(25)20-17(26)19-15)5-11(22)14(24)12(23)6-30-31(27,28)29;/h3-4,11-12,14,22-24H,5-6H2,1-2H3,(H,20,25,26)(H2,27,28,29);/q;+1/p-1/t11-,12+,14-;/m0./s1

SMILES Code

O=P(OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=CC(C)=C(C)C=C2N=C3C(NC(N=C13)=O)=O)([O-])O.[Na+]

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Riboflavin is a precursor of the coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). These coenzymes are of vital importance in normal tissue respiration, pyridoxine activation, tryptophan to niacin conversion, fat, carbohydrate, and protein metabolism, and glutathione reductase mediated detoxification. Riboflavin may also be involved in maintaining erythrocyte integrity. This vitamin is essential for healthy skin, nails, and hair.

In vitro activity:

In the HepG2 cell line, riboflavin deficiency induces cell apoptosis by triggering ER stress and the CHOP pathway. Riboflavin deficiency inhibited cell proliferation and caused ER stress, increased the expression of ER stress markers, increased the cell apoptosis rate, enhanced the expression of proapoptotic markers, and decreased the expression of the antiapoptotic marker. Reference: Nutrients. 2022 Aug 16;14(16):3356. https://pubmed.ncbi.nlm.nih.gov/36014863/

In vivo activity:

In an arsenic-injured rat model, it was revealed that riboflavin supplementation protected testicular structures against As2O3-induced injury via a dual inhibition of oxidative changes and a regulation of the PINK1-mediated pathway. Exposure to riboflavin led to a significant decrease of the expression levels of mitophagy related genes. Reference: Physiol Res. 2021 Aug 31;70(4):591-603. https://pubmed.ncbi.nlm.nih.gov/34062077/

	Solvent	mg/mL	mM
Solubility
	DMSO	1.0	2.09
	Water	20.8	43.55

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 478.32 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Zhang B, Cao JT, Wu YB, Gao KX, Xie M, Zhou ZK, Tang J, Hou SS. Riboflavin (Vitamin B2) Deficiency Induces Apoptosis Mediated by Endoplasmic Reticulum Stress and the CHOP Pathway in HepG2 Cells. Nutrients. 2022 Aug 16;14(16):3356. doi: 10.3390/nu14163356. PMID: 36014863; PMCID: PMC9414855. 2. Long L, Pang XX, Zeng FM, Zhan XH, Xie YH, Pan F, Wang W, Liao LD, Xu XE, Li B, Wang LD, Chang ZJ, Li EM, Xu LY. Promotion of rs3746804 (p. L267P) polymorphism to intracellular SLC52A3a trafficking and riboflavin transportation in esophageal cancer cells. Amino Acids. 2021 Aug;53(8):1197-1209. doi: 10.1007/s00726-021-03025-4. Epub 2021 Jul 5. PMID: 34223992. 3. Olfati A, Tvrda E. Riboflavin recovery of spermatogenic dysfunction via a dual inhibition of oxidative changes and regulation of the PINK1-mediated pathway in arsenic-injured rat model. Physiol Res. 2021 Aug 31;70(4):591-603. doi: 10.33549/physiolres.934658. Epub 2021 Jun 1. PMID: 34062077; PMCID: PMC8820542. 4. Alhazza IM, Hassan I, Ebaid H, Al-Tamimi J, Alwasel SH. Chemopreventive effect of riboflavin on the potassium bromate-induced renal toxicity in vivo. Naunyn Schmiedebergs Arch Pharmacol. 2020 Dec;393(12):2355-2364. doi: 10.1007/s00210-020-01938-7. Epub 2020 Jul 14. PMID: 32666286.

In vitro protocol:

In vivo protocol:

1. Olfati A, Tvrda E. Riboflavin recovery of spermatogenic dysfunction via a dual inhibition of oxidative changes and regulation of the PINK1-mediated pathway in arsenic-injured rat model. Physiol Res. 2021 Aug 31;70(4):591-603. doi: 10.33549/physiolres.934658. Epub 2021 Jun 1. PMID: 34062077; PMCID: PMC8820542. 2. Alhazza IM, Hassan I, Ebaid H, Al-Tamimi J, Alwasel SH. Chemopreventive effect of riboflavin on the potassium bromate-induced renal toxicity in vivo. Naunyn Schmiedebergs Arch Pharmacol. 2020 Dec;393(12):2355-2364. doi: 10.1007/s00210-020-01938-7. Epub 2020 Jul 14. PMID: 32666286.

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