MSAB | CAS#173436-66-3 | Wnt/β-catenin signaling inhibitor

MedKoo Cat#: 561321 | Name: MSAB

Description:

WARNING: This product is for research use only, not for human or veterinary use.

MSAB is a selective inhibitor of Wnt/β-catenin signaling. MSAB shows potent anti-tumor effects selectively on Wnt-dependent cancer cells in vitro and in mouse cancer models. MSAB binds to β-catenin, promoting its degradation, and specifically downregulates Wnt/β-catenin target genes.

Chemical Structure

MSAB

CAS#173436-66-3

Theoretical Analysis

MedKoo Cat#: 561321

Name: MSAB

CAS#: 173436-66-3

Chemical Formula: C15H15NO4S

Exact Mass: 305.0722

Molecular Weight: 305.35

Elemental Analysis: C, 59.00; H, 4.95; N, 4.59; O, 20.96; S, 10.50

Price and Availability

Size	Price	Availability
50mg	USD 150.00	Ready to ship
100mg	USD 225.00	Ready to ship
200mg	USD 385.00	Ready to ship
500mg	USD 825.00	Ready to ship
1g	USD 1,350.00	Ready to ship
2g	USD 2,150.00	Ready to ship

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Related CAS #

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Synonym

MSAB;

IUPAC/Chemical Name

Methyl 3-{[(4-methylphenyl)sulfonyl]amino}benzoate

InChi Key

CVKBYFCJQSPBOI-UHFFFAOYSA-N

InChi Code

InChI=1S/C15H15NO4S/c1-11-6-8-14(9-7-11)21(18,19)16-13-5-3-4-12(10-13)15(17)20-2/h3-10,16H,1-2H3

SMILES Code

O=C(OC)C1=CC=CC(NS(=O)(C2=CC=C(C)C=C2)=O)=C1

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot#A8T05K14

QC Data

View QC data: current batch, Lot#A8T05K14

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

MSAB is an inhibitor of Wnt/β-catenin signaling.

In vitro activity:

Further, to examine MsaB regulation of ccpE and gudB, this study performed an EMSA, which showed MsaB binding to the ccpE promoter (Figure 5B) and thereby suggested that MsaB regulates the TCA cycle via ccpE. This also confirms previous finding of MsaB regulation of ccpE in the UAMS-1 strain. However, the absence of binding to the promoter of gudB suggests that its regulation by MsaB might be indirect (Figure 5C). All these observations lead this study to conclude that MsaB directly regulates ccpE to control TCA cycle activity and that the increased energy state in the △msaABCR led to decreased persister cell formation in late exponential growth phase. Reference: Front Microbiol. 2021; 12: 657753. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079656/

In vivo activity:

Catenin is the mediator of canonical Wnt signalling, and levels of active β-catenin can be reduced using the inhibitor MSAB. This is a cell-permeable compound that binds to β-catenin and induces its ubiquitination and proteasomal degradation. When HCT116 cells were treated with MSAB, there was a reduction in active β-catenin and TNS4 levels were also downregulated (Figure 4A). Reference: Int J Exp Pathol. 2020 Jun;101(3-4):80-86. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370848/

	Solvent	mg/mL	mM
Solubility
	Soluble in DMSO	25.0	81.90

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 305.35 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Pandey S, Sahukhal GS, Elasri MO. The msaABCR Operon Regulates Persister Formation by Modulating Energy Metabolism in Staphylococcus aureus. Front Microbiol. 2021 Apr 14;12:657753. doi: 10.3389/fmicb.2021.657753. PMID: 33936014; PMCID: PMC8079656. 2. Pal A, Ashworth JC, Collier P, Probert C, Jones S, Leza EP, Meakin ML, A Ritchie A, Onion D, Clarke PA, Allegrucci C, Grabowska AM. A 3D Heterotypic Breast Cancer Model Demonstrates a Role for Mesenchymal Stem Cells in Driving a Proliferative and Invasive Phenotype. Cancers (Basel). 2020 Aug 14;12(8):2290. doi: 10.3390/cancers12082290. PMID: 32824003; PMCID: PMC7465555. 3. Raposo TP, Alfahed A, Nateri AS, Ilyas M. Tensin4 (TNS4) is upregulated by Wnt signalling in adenomas in multiple intestinal neoplasia (Min) mice. Int J Exp Pathol. 2020 Jun;101(3-4):80-86. doi: 10.1111/iep.12352. Epub 2020 Jun 22. PMID: 32567731; PMCID: PMC7370848.

In vitro protocol:

In vivo protocol:

1. Raposo TP, Alfahed A, Nateri AS, Ilyas M. Tensin4 (TNS4) is upregulated by Wnt signalling in adenomas in multiple intestinal neoplasia (Min) mice. Int J Exp Pathol. 2020 Jun;101(3-4):80-86. doi: 10.1111/iep.12352. Epub 2020 Jun 22. PMID: 32567731; PMCID: PMC7370848.

1: Hwang SY, Deng X, Byun S, Lee C, Lee SJ, Suh H, Zhang J, Kang Q, Zhang T, Westover KD, Mandinova A, Lee SW. Direct Targeting of β-Catenin by a Small Molecule Stimulates Proteasomal Degradation and Suppresses Oncogenic Wnt/β-Catenin Signaling. Cell Rep. 2016 Jun 28;16(1):28-36. doi: 10.1016/j.celrep.2016.05.071. Epub 2016 Jun 16. PubMed PMID: 27320923; PubMed Central PMCID: PMC4957947.