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MedKoo Cat#: 571053 | Name: Nephrin

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Nephrin is a cell surface signaling receptor protein that regulates podocyte function and plays a role in β-cell survival signaling; it is a protein necessary for the proper functioning of the renal filtration barrier. Nephrin belongs to a family of highly conserved proteins with a well characterized function as modulators of cell adhesion and guidance, and nephrin may have a role in metabolic pathways linked to podocyte and pancreatic β-cell survival. Nephrin is associated and partly co-localized with PI3-kinase in mouse islet β-cells and the mouse pancreatic beta-cell line (βTC-6 cells). Nephrin silencing abolishes Akt activation and increased susceptibility of cells to apoptosis. Glucose-induced changes in nephrin signaling may contribute to gradual pancreatic β-cell loss in type 2 diabetes. This suggests a role for nephrin as an independent modulator of podocyte and pancreatic β-cell nutrient sensing in the fasting state and the potential of nephrin as a drug target in diabetes.

Chemical Structure

Nephrin
Nephrin
CAS#65136-96-1

Theoretical Analysis

MedKoo Cat#: 571053

Name: Nephrin

CAS#: 65136-96-1

Chemical Formula: C30H52O3

Exact Mass: 460.3916

Molecular Weight: 460.74

Elemental Analysis: C, 78.21; H, 11.38; O, 10.42

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.
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Related CAS #
No Data
Synonym
Nephrin
IUPAC/Chemical Name
Hopane-6alpha,7beta,22-triol
InChi Key
VYLJAYXZTOTZRR-GQZYKZBKSA-N
InChi Code
InChI=1S/C30H52O3/c1-25(2)14-9-15-28(6)21-11-10-20-27(5)16-12-18(26(3,4)33)19(27)13-17-29(20,7)30(21,8)24(32)22(31)23(25)28/h18-24,31-33H,9-17H2,1-8H3/t18-,19-,20+,21+,22+,23?,24-,27-,28+,29+,30-/m0/s1
SMILES Code
CC1(C)CCC[C@]2(C)[C@@]3([H])CC[C@]4([H])[C@@]5(C)CC[C@H](C(C)(O)C)[C@@]([H])5CC[C@](C)4[C@@](C)3[C@@H](O)[C@H](O)C12
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 460.74 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Li X, Chuang PY, D'Agati VD, Dai Y, Yacoub R, Fu J, Xu J, Taku O, Premsrirut PK, Holzman LB, He JC. Nephrin Preserves Podocyte Viability and Glomerular Structure and Function in Adult Kidneys. J Am Soc Nephrol. 2015 Oct;26(10):2361-77. doi: 10.1681/ASN.2014040405. Epub 2015 Feb 2. PubMed PMID: 25644109; PubMed Central PMCID: PMC4587684. 2: Li X, He JC. An update: the role of Nephrin inside and outside the kidney. Sci China Life Sci. 2015 Jul;58(7):649-57. doi: 10.1007/s11427-015-4844-1. Epub 2015 Apr 29. Review. PubMed PMID: 25921941. 3: Kapodistria K, Tsilibary EP, Politis P, Moustardas P, Charonis A, Kitsiou P. Nephrin, a transmembrane protein, is involved in pancreatic beta-cell survival signaling. Mol Cell Endocrinol. 2015 Jan 15;400:112-28. doi: 10.1016/j.mce.2014.11.003. Epub 2014 Nov 28. PubMed PMID: 25448064. 4: Wang P, Li M, Liu Q, Chen B, Ji Z. Detection of urinary podocytes and nephrin as markers for children with glomerular diseases. Exp Biol Med (Maywood). 2015 Feb;240(2):169-74. doi: 10.1177/1535370214548995. Epub 2014 Sep 21. PubMed PMID: 25245074; PubMed Central PMCID: PMC4935320. 5: Verma R, Venkatareddy M, Kalinowski A, Patel SR, Salant DJ, Garg P. Shp2 Associates with and Enhances Nephrin Tyrosine Phosphorylation and Is Necessary for Foot Process Spreading in Mouse Models of Podocyte Injury. Mol Cell Biol. 2015 Dec 7;36(4):596-614. doi: 10.1128/MCB.00956-15. PubMed PMID: 26644409; PubMed Central PMCID: PMC4751697. 6: Lin CL, Lee PH, Hsu YC, Lei CC, Ko JY, Chuang PC, Huang YT, Wang SY, Wu SL, Chen YS, Chiang WC, Reiser J, Wang FS. MicroRNA-29a promotion of nephrin acetylation ameliorates hyperglycemia-induced podocyte dysfunction. J Am Soc Nephrol. 2014 Aug;25(8):1698-709. doi: 10.1681/ASN.2013050527. Epub 2014 Feb 27. PubMed PMID: 24578127; PubMed Central PMCID: PMC4116051. 7: Li Y, Zheng R, Wang R, Lu X, Zhu C, Lin HY, Wang H, Yu X, Fu J. Involvement of nephrin in human placental trophoblast syncytialization. Reproduction. 2015 Apr;149(4):339-46. doi: 10.1530/REP-14-0424. Epub 2015 Jan 22. PubMed PMID: 25614620. 8: Ristola M, Lehtonen S. Functions of the podocyte proteins nephrin and Neph3 and the transcriptional regulation of their genes. Clin Sci (Lond). 2014 Mar;126(5):315-28. doi: 10.1042/CS20130258. Review. PubMed PMID: 24219158. 9: Liu Y, Liang W, Yang Y, Pan Y, Yang Q, Chen X, Singhal PC, Ding G. IQGAP1 regulates actin cytoskeleton organization in podocytes through interaction with nephrin. Cell Signal. 2015 Apr;27(4):867-77. doi: 10.1016/j.cellsig.2015.01.015. Epub 2015 Jan 31. PubMed PMID: 25652011; PubMed Central PMCID: PMC4356988. 10: Denhez B, Lizotte F, Guimond MO, Jones N, Takano T, Geraldes P. Increased SHP-1 protein expression by high glucose levels reduces nephrin phosphorylation in podocytes. J Biol Chem. 2015 Jan 2;290(1):350-8. doi: 10.1074/jbc.M114.612721. Epub 2014 Nov 17. PubMed PMID: 25404734; PubMed Central PMCID: PMC4281738. 11: Fukuyo Y, Nakamura T, Bubenshchikova E, Powell R, Tsuji T, Janknecht R, Obara T. Nephrin and Podocin functions are highly conserved between the zebrafish pronephros and mammalian metanephros. Mol Med Rep. 2014 Feb;9(2):457-65. doi: 10.3892/mmr.2013.1844. Epub 2013 Dec 6. PubMed PMID: 24337247; PubMed Central PMCID: PMC3896505. 12: Lin X, You Y, Wang J, Qin Y, Huang P, Yang F. MicroRNA-155 deficiency promotes nephrin acetylation and attenuates renal damage in hyperglycemia-induced nephropathy. Inflammation. 2015 Apr;38(2):546-54. doi: 10.1007/s10753-014-9961-7. PubMed PMID: 24969676. 13: Yu M, Ren Q, Yu SY. Role of nephrin phosphorylation inducted by dexamethasone and angiotensin II in podocytes. Mol Biol Rep. 2014 Jun;41(6):3591-5. doi: 10.1007/s11033-014-3222-6. Epub 2014 Feb 11. PubMed PMID: 24515388. 14: New LA, Martin CE, Scott RP, Platt MJ, Keyvani Chahi A, Stringer CD, Lu P, Samborska B, Eremina V, Takano T, Simpson JA, Quaggin SE, Jones N. Nephrin Tyrosine Phosphorylation Is Required to Stabilize and Restore Podocyte Foot Process Architecture. J Am Soc Nephrol. 2016 Aug;27(8):2422-35. doi: 10.1681/ASN.2015091048. Epub 2016 Jan 22. PubMed PMID: 26802179; PubMed Central PMCID: PMC4978059. 15: Villarreal R, Mitrofanova A, Maiguel D, Morales X, Jeon J, Grahammer F, Leibiger IB, Guzman J, Fachado A, Yoo TH, Busher Katin A, Gellermann J, Merscher S, Burke GW, Berggren PO, Oh J, Huber TB, Fornoni A. Nephrin Contributes to Insulin Secretion and Affects Mammalian Target of Rapamycin Signaling Independently of Insulin Receptor. J Am Soc Nephrol. 2016 Apr;27(4):1029-41. doi: 10.1681/ASN.2015020210. Epub 2015 Sep 23. PubMed PMID: 26400569; PubMed Central PMCID: PMC4814188. 16: Verma R, Venkatareddy M, Kalinowski A, Patel SR, Garg P. Integrin Ligation Results in Nephrin Tyrosine Phosphorylation In Vitro. PLoS One. 2016 Feb 5;11(2):e0148906. doi: 10.1371/journal.pone.0148906. eCollection 2016. PubMed PMID: 26848974; PubMed Central PMCID: PMC4743922. 17: Yun BH, Lee SM, Cho HY, Kim JY, Son GH, Kim YH, Park YW, Lim BJ, Kwon JY. Expression of nephrin in the human placenta and fetal membranes. Mol Med Rep. 2015 Oct;12(4):5116-20. doi: 10.3892/mmr.2015.4044. Epub 2015 Jul 6. PubMed PMID: 26151763. 18: Enatsu N, Miyake H, Chiba K, Fujisawa M. Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin. Biomed Res Int. 2015;2015:273784. doi: 10.1155/2015/273784. Epub 2015 Oct 11. PubMed PMID: 26539476; PubMed Central PMCID: PMC4619786. 19: Oranskiy SP, Yeliseyeva LN. Urinary excretion of nephrin in rheumatoid arthritis patients with proteinuria. Clin Exp Rheumatol. 2014 Jul-Aug;32(4):572-4. Epub 2014 May 21. PubMed PMID: 24848282. 20: Ichimura K, Fukuyo Y, Nakamura T, Powell R, Sakai T, Janknecht R, Obara T. Developmental localization of nephrin in zebrafish and medaka pronephric glomerulus. J Histochem Cytochem. 2013 Apr;61(4):313-24. doi: 10.1369/0022155413477115. Epub 2013 Jan 15. PubMed PMID: 23324868; PubMed Central PMCID: PMC3636682.