MedKoo Cat#: 561018 | Name: Sulforaphene
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Sulforaphene efficiently decreased the viability of breast cancer cells, while normal cells (MCF10A) were less sensitive to the analyzed isothiocyanate. Sulforaphene induced G2/M cell cycle arrest, as well as disturbed cytoskeletal organization and reduced clonogenic potential of the cancer cells. Sulforaphene induced apoptosis in a concentration-dependent manner which was associated with the oxidative stress, mitochondria dysfunction, increased Bax:Bcl2 ratio and ADRP levels.

Chemical Structure

Sulforaphene
Sulforaphene
CAS#592-95-0

Theoretical Analysis

MedKoo Cat#: 561018

Name: Sulforaphene

CAS#: 592-95-0

Chemical Formula: C6H9NOS2

Exact Mass: 175.0126

Molecular Weight: 175.26

Elemental Analysis: C, 41.12; H, 5.18; N, 7.99; O, 9.13; S, 36.58

Price and Availability

Size Price Availability Quantity
1mg USD 300.00
5mg USD 600.00
10mg USD 900.00
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
Sulforaphene; Raphanin; Sulforaphen; Sulphoraphen
IUPAC/Chemical Name
4-Isothiocyanato-1-(methylsulfinyl)-1-butene
InChi Key
QKGJFQMGPDVOQE-HWKANZROSA-N
InChi Code
InChI=1S/C6H9NOS2/c1-10(8)5-3-2-4-7-6-9/h3,5H,2,4H2,1H3/b5-3+
SMILES Code
O=S(/C=C/CCN=C=S)C
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Biological target:
Sulforaphene has an ED50 against velvetleaf seedlings approximately 2 x 10-4 M
In vitro activity:
Sulforaphene significantly suppressed ESCC cell proliferation in vitro and reduced the tumour size in an oesophageal patient-derived xenograft (PDX) SCID mouse model. Furthermore, the binding of sulforaphane to MSK2 in vitro was verified using a cellular thermal dhift assay, and the effect of MSK2 knockdown on the ESCC phenotype was observed using a shMSK2 model. Reference: Zhang C, Wu Q, Yao K, Jin G, Zhao S, Zhang J, Zheng W, Xu B, Zu Y, Yuan J, Liu K, Guo Y. Sulforaphene suppresses oesophageal cancer growth through mitogen- and stress-activated kinase 2 in a PDX mouse model. Am J Cancer Res. 2023 Oct 15;13(10):4708-4720. PMID: 37970356; PMCID: PMC10636680.
In vivo activity:
Through in vitro and in vivo experiments, our study demonstrated that SFE effectively inhibits M1 polarization while promoting M2 polarization, ultimately leading to the suppression of periodontitis. Transcriptome sequencing showed that SFE significantly upregulated the expression of dendritic cell immunoreceptor (DCIR, also known as CLEC4A2). Reference: Liao Y, Yan Q, Cheng T, Yao H, Zhao Y, Fu D, Ji Y, Shi B. Sulforaphene Inhibits Periodontitis through Regulating Macrophage Polarization via Upregulating Dendritic Cell Immunoreceptor. J Agric Food Chem. 2023 Oct 25;71(42):15538-15552. doi: 10.1021/acs.jafc.3c02619. Epub 2023 Oct 12. PMID: 37823224.
Solvent mg/mL mM
Solubility
DMSO 100.0 570.55
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 175.26 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
1: Zhang C, Wu Q, Yao K, Jin G, Zhao S, Zhang J, Zheng W, Xu B, Zu Y, Yuan J, Liu K, Guo Y. Sulforaphene suppresses oesophageal cancer growth through mitogen- and stress-activated kinase 2 in a PDX mouse model. Am J Cancer Res. 2023 Oct 15;13(10):4708-4720. PMID: 37970356; PMCID: PMC10636680. 2: Zhou Y, Zhang B, Wang Y, Hu R. Effects of Sulforaphene on the Cariogenic Properties of Streptococcus Mutans In Vitro and Dental Caries Development In Vivo. Antibiotics (Basel). 2023 Aug 24;12(9):1359. doi: 10.3390/antibiotics12091359. PMID: 37760656; PMCID: PMC10525627.
In vivo protocol:
1: Liao Y, Yan Q, Cheng T, Yao H, Zhao Y, Fu D, Ji Y, Shi B. Sulforaphene Inhibits Periodontitis through Regulating Macrophage Polarization via Upregulating Dendritic Cell Immunoreceptor. J Agric Food Chem. 2023 Oct 25;71(42):15538-15552. doi: 10.1021/acs.jafc.3c02619. Epub 2023 Oct 12. PMID: 37823224. 2: Zhang B, Liu P, Sheng H, Guo Y, Han Y, Suo L, Yuan Q. New Insight into the Potential Protective Function of Sulforaphene against ROS-Mediated Oxidative Stress Damage In Vitro and In Vivo. Int J Mol Sci. 2023 Aug 23;24(17):13129. doi: 10.3390/ijms241713129. PMID: 37685936; PMCID: PMC10487408.
1: Gao R, Liu P, Bi J, Jiang Y, Zhao T, Yuan X, Zhang C, Wang Y. The Effects of Different Thiol-Containing Compounds on the Degradation of Sulforaphene. Molecules. 2024 Sep 12;29(18):4328. doi: 10.3390/molecules29184328. PMID: 39339323; PMCID: PMC11434082. 2: Zhu Q, Zhang P, Liu D, Tang L, Yu J, Zhang C, Jiang G. Glucosinolate extract from radish (Raphanus sativus L.) seed attenuates high-fat diet-induced obesity: insights into gut microbiota and fecal metabolites. Front Nutr. 2024 Aug 8;11:1442535. doi: 10.3389/fnut.2024.1442535. PMID: 39176030; PMCID: PMC11340518. 3: Zhao Z, Yuan Y, Li S, Wang X, Yang X. Natural compounds from herbs and nutraceuticals as glycogen synthase kinase-3β inhibitors in Alzheimer's disease treatment. CNS Neurosci Ther. 2024 Aug;30(8):e14885. doi: 10.1111/cns.14885. PMID: 39129397; PMCID: PMC11317746. 4: Qu Y, Li X, Chen X, Li J, Yu Z, Shen R. Novel pH-sensitive gellan gum-ε- polylysine hydrogel microspheres for sulforaphene delivery. J Sci Food Agric. 2024 Dec;104(15):9423-9433. doi: 10.1002/jsfa.13765. Epub 2024 Jul 24. PMID: 39045717. 5: Qu Y, Li X, Li J, Yu Z, Shen R. Combining network pharmacology and experimental verification to study the anti-colon cancer effect and mechanism of sulforaphene. J Sci Food Agric. 2024 Nov;104(14):8769-8779. doi: 10.1002/jsfa.13703. Epub 2024 Jul 18. PMID: 39023003. 6: Liang PY, Huang YJ, Chen HM, Cao HH, Wu YB, Chen TT, Chen Y, Fan HX, Zheng JX. [Research progress in raw and processed Raphani Semen and prediction of quality markers]. Zhongguo Zhong Yao Za Zhi. 2024 Apr;49(8):2076-2087. Chinese. doi: 10.19540/j.cnki.cjcmm.20231219.201. PMID: 38812224. 7: Yamaguchi Y, Sugiki M, Shimizu M, Ogawa K, Kumagai H. Comparative analysis of isothiocyanates in eight cruciferous vegetables and evaluation of the hepatoprotective effects of 4-(methylsulfinyl)-3-butenyl isothiocyanate (sulforaphene) from daikon radish (Raphanus sativus L.) sprouts. Food Funct. 2024 May 7;15(9):4894-4904. doi: 10.1039/d4fo00133h. PMID: 38597802. 8: Zhang B, Liu P, Sheng H, Han Y, Yuan Q. Long-term intake of sulforaphene alleviates D-galactose-induced skin senescence by activating AMPK-Sirt 1 pathway. Mol Cell Biochem. 2024 Mar 5. doi: 10.1007/s11010-024-04965-7. Epub ahead of print. PMID: 38441777. 9: Che G, Chen M, Li X, Xiao J, Liu L, Guo L. Effect of UV-A Irradiation on Bioactive Compounds Accumulation and Hypoglycemia-Related Enzymes Activities of Broccoli and Radish Sprouts. Plants (Basel). 2024 Feb 3;13(3):450. doi: 10.3390/plants13030450. PMID: 38337982; PMCID: PMC10857714. 10: Hur GH, Lee TK, Cho YJ, Kim JH, Park JHY, Yang H, Lee KW. Optimization of cultivar, germination time and extraction for radish sprout extract with high sulforaphene content. J Sci Food Agric. 2024 Jul;104(9):5010-5020. doi: 10.1002/jsfa.13357. Epub 2024 Feb 23. PMID: 38314949. 11: Yu HY, Yang L, Liu YC, Yu AJ. Sulforaphene suppressed cell proliferation and promoted apoptosis of COV362 cells in endometrioid ovarian cancer. PeerJ. 2023 Nov 21;11:e16308. doi: 10.7717/peerj.16308. PMID: 38025760; PMCID: PMC10668859. 12: Zhang C, Wu Q, Yao K, Jin G, Zhao S, Zhang J, Zheng W, Xu B, Zu Y, Yuan J, Liu K, Guo Y. Sulforaphene suppresses oesophageal cancer growth through mitogen- and stress-activated kinase 2 in a PDX mouse model. Am J Cancer Res. 2023 Oct 15;13(10):4708-4720. PMID: 37970356; PMCID: PMC10636680. 13: Liu H, Liu M, Tian X, Wang H, Gao J, Li H, Zhao Z, Liu Y, Liu C, Chen X, Yang Y. Discovery and biological evaluation of a potent small molecule CRM1 inhibitor for its selective ablation of extranodal NK/T cell lymphoma. Elife. 2023 Oct 27;12:e80625. doi: 10.7554/eLife.80625. PMID: 37888961; PMCID: PMC10637774. 14: Liao Y, Yan Q, Cheng T, Yao H, Zhao Y, Fu D, Ji Y, Shi B. Sulforaphene Inhibits Periodontitis through Regulating Macrophage Polarization via Upregulating Dendritic Cell Immunoreceptor. J Agric Food Chem. 2023 Oct 25;71(42):15538-15552. doi: 10.1021/acs.jafc.3c02619. Epub 2023 Oct 12. PMID: 37823224. 15: Zhou Y, Zhang B, Wang Y, Hu R. Effects of Sulforaphene on the Cariogenic Properties of Streptococcus Mutans In Vitro and Dental Caries Development In Vivo. Antibiotics (Basel). 2023 Aug 24;12(9):1359. doi: 10.3390/antibiotics12091359. PMID: 37760656; PMCID: PMC10525627. 16: Yu X, Gao L, Miao Z, Zhang L, Wu R, Sun S, Sun N, Zhu L, Sheng H. Qualitative and quantitative analysis of the component variations of Raphani Semen during the stir-frying process and elucidation of transformation pathways of multiple components. J Pharm Biomed Anal. 2023 Nov 30;236:115726. doi: 10.1016/j.jpba.2023.115726. Epub 2023 Sep 17. PMID: 37729746. 17: Zhang B, Liu P, Sheng H, Guo Y, Han Y, Suo L, Yuan Q. New Insight into the Potential Protective Function of Sulforaphene against ROS-Mediated Oxidative Stress Damage In Vitro and In Vivo. Int J Mol Sci. 2023 Aug 23;24(17):13129. doi: 10.3390/ijms241713129. PMID: 37685936; PMCID: PMC10487408. 18: Lee TK, Hur G, Kim JH, Park JHY, Yang H, Lee KW. Micro-grinding-based production for sulforaphene-enriched radish seeds extract via facilitating glucosinolates-myrosinase reaction, and evaluation of its anti-adipogenic effects. Food Chem. 2023 Dec 15;429:136864. doi: 10.1016/j.foodchem.2023.136864. Epub 2023 Jul 16. PMID: 37506660. 19: Yao H, Du Y, Jiang B, Liao Y, Zhao Y, Yin M, Li T, Sheng Y, Ji Y, Du M. Sulforaphene suppresses RANKL-induced osteoclastogenesis and LPS-induced bone erosion by activating Nrf2 signaling pathway. Free Radic Biol Med. 2023 Oct;207:48-62. doi: 10.1016/j.freeradbiomed.2023.07.009. Epub 2023 Jul 7. PMID: 37423561. 20: Lee TK, Hur G, Choi J, Ban C, Kim JY, Yang H, Park JHY, Lee KW, Kim JH. Enhancing stability and bioavailability of sulforaphene in radish seed extracts using nanoemulsion made with high oleic sunflower oil. Food Sci Biotechnol. 2023 Apr 4;32(9):1269-1279. doi: 10.1007/s10068-023-01304-2. PMID: 37362810; PMCID: PMC10290006.