Clomipramine Hydrochloride | CAS#17321-77-6 | mAChRs Inhibitor |5-HT2/3/6/7 Receptor Inhibitor | α1/2-Adrenergic Aeceptor Inhibitor

MedKoo Cat#: 540083 | Name: Clomipramine HCl

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Clomipramine HCl inhibitor of mAChRs, 5-HT2/3/6/7 receptors, α1/2-adrenergic receptors, SERT, NET, and hERG K and L-type Ca channels used to treat depression, anxiety, narcolepClomipramine HCl sy, and obessive-compulisve disorder. It also acts as a FIASMA, decreases thermal and mechanical pain, and may prolong the cardiac QT interval.

Chemical Structure

Clomipramine HCl

CAS#17321-77-6 (HCl)

Theoretical Analysis

MedKoo Cat#: 540083

Name: Clomipramine HCl

CAS#: 17321-77-6 (HCl)

Chemical Formula: C19H24Cl2N2

Exact Mass: 0.0000

Molecular Weight: 351.31

Elemental Analysis: C, 64.96; H, 6.89; Cl, 20.18; N, 7.97

Price and Availability

Size	Price	Availability
1g	USD 150.00	2 Weeks
2g	USD 250.00	2 Weeks
5g	USD 450.00	2 Weeks

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Related CAS #

17321-77-6 (HCl) 303-49-1 (free base)

Synonym

Anafranil; Clomicalm; Chlorimipramine HCl; 3-Chloroimipramine HCl; Chloroimipramine monohydrochloride; G-34586; G34586; G 34586; NSC-759323; NSC 759323; NSC759323; Hydiphen

IUPAC/Chemical Name

3-(3-chloro-10,11-dihydro-5H-dibenzo[b,f]azepin-5-yl)-N,N-dimethylpropan-1-amine hydrochloride

InChi Key

WIMWMKZEIBHDTH-UHFFFAOYSA-N

InChi Code

InChI=1S/C19H23ClN2.ClH/c1-21(2)12-5-13-22-18-7-4-3-6-15(18)8-9-16-10-11-17(20)14-19(16)22;/h3-4,6-7,10-11,14H,5,8-9,12-13H2,1-2H3;1H

SMILES Code

CN(C)CCCN1C2=CC(Cl)=CC=C2CCC3=CC=CC=C31.[H]Cl

Appearance

White to off-white crystalline powder.

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Clomipramine hydrochloride is a serotonin transporter (SERT), norepinephrine transporter (NET) and dopamine transporter (DAT) blocker with Ki of 0.14, 54 and 3 nM, respectively.

In vitro activity:

Regarding the mechanism of action, clomipramine led parasites do mitochondrial depolarization, which coupled with the inhibition of trypanothione reductase induced strong oxidative stress in the parasites. The effects observed in promastigotes included lipoperoxidation, plasma membrane permeabilization, and apoptosis hallmarks (i.e., DNA fragmentation, phosphatidylserine exposure, and cell shrinkage). Reference: Parasitol Res. 2019 Mar;118(3):977-989. https://pubmed.ncbi.nlm.nih.gov/30694414/

In vivo activity:

Clomipramine-treated rats had significantly lower rates of glycolysis and glycolytic capacity, regardless of sex. Coupling efficiency was significantly higher in male rats compared with female rats across treatment groups. Reference: Physiol Rep. 2018 May;6(9):e13615. https://pubmed.ncbi.nlm.nih.gov/29745454/

	Solvent	mg/mL	mM
Solubility
	DMSO	43.3	123.34
	DMF	3.0	8.54
	Ethanol	40.0	113.86
	PBS (pH 7.2)	0.5	1.42
	Water	60.0	170.79

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 351.31 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. da Silva Rodrigues JH, Miranda N, Volpato H, Ueda-Nakamura T, Nakamura CV. The antidepressant clomipramine induces programmed cell death in Leishmania amazonensis through a mitochondrial pathway. Parasitol Res. 2019 Mar;118(3):977-989. doi: 10.1007/s00436-018-06200-x. Epub 2019 Jan 29. PMID: 30694414. 2. Jimenez AG, Winward JD, Smith DM, Ragan CM. Effects of short-term clomipramine on anxiety-like behavior, cellular metabolism, and oxidative stress in primary fibroblast cells of male and female rats. Physiol Rep. 2018 May;6(9):e13615. doi: 10.14814/phy2.13615. PMID: 29745454; PMCID: PMC5943669. 3. Li X, Sun W, Li J, Wang M, Zhang H, Pei L, Boyce BF, Wang Z, Xing L. Clomipramine causes osteoporosis by promoting osteoclastogenesis via E3 ligase Itch, which is prevented by Zoledronic acid. Sci Rep. 2017 Feb 1;7:41358. doi: 10.1038/srep41358. PMID: 28145497; PMCID: PMC5286409.

In vitro protocol:

In vivo protocol:

1. Jimenez AG, Winward JD, Smith DM, Ragan CM. Effects of short-term clomipramine on anxiety-like behavior, cellular metabolism, and oxidative stress in primary fibroblast cells of male and female rats. Physiol Rep. 2018 May;6(9):e13615. doi: 10.14814/phy2.13615. PMID: 29745454; PMCID: PMC5943669. 2. Li X, Sun W, Li J, Wang M, Zhang H, Pei L, Boyce BF, Wang Z, Xing L. Clomipramine causes osteoporosis by promoting osteoclastogenesis via E3 ligase Itch, which is prevented by Zoledronic acid. Sci Rep. 2017 Feb 1;7:41358. doi: 10.1038/srep41358. PMID: 28145497; PMCID: PMC5286409.

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