NIH-12848 | CAS#959551-10-1 | PI5P4Kγ) inhibitor

MedKoo Cat#: 530601 | Name: NIH-12848

Description:

WARNING: This product is for research use only, not for human or veterinary use.

NIH-12848 is a putative phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) inhibitor. NIH-12848 inhibited PI5P4Kγ with an IC50 of approximately 1 μM but did not inhibit the α and β PI5P4K isoforms at concentrations up to 100 μM. NIH-12848 is a potentially powerful tool for exploring the cell physiology of PI5P4Ks.

Chemical Structure

NIH-12848

CAS#959551-10-1

Theoretical Analysis

MedKoo Cat#: 530601

Name: NIH-12848

CAS#: 959551-10-1

Chemical Formula: C20H14F3N3S

Exact Mass: 385.0861

Molecular Weight: 385.41

Elemental Analysis: C, 62.33; H, 3.66; F, 14.79; N, 10.90; S, 8.32

Price and Availability

Size	Price	Availability	Quantity
100mg	USD 850.00
200mg	USD 1,250.00
500mg	USD 2,150.00
1g	USD 3,450.00

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Synonym

NIH-12848; NIH 12848; NIH12848, NCGC00012848-02;

IUPAC/Chemical Name

N-(thiophen-2-ylmethyl)-2-(2-(trifluoromethyl)phenyl)quinazolin-4-amine

InChi Key

QBDAEJRHUCSSPR-UHFFFAOYSA-N

InChi Code

InChI=1S/C20H14F3N3S/c21-20(22,23)16-9-3-1-7-14(16)19-25-17-10-4-2-8-15(17)18(26-19)24-12-13-6-5-11-27-13/h1-11H,12H2,(H,24,25,26)

SMILES Code

FC(F)(F)C(C=CC=C1)=C1C2=NC3=CC=CC=C3C(NCC4=CC=CS4)=N2

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

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Product Data

Product Data

Instruction

View handling instruction

Biological target:

NIH-12848 is a putative phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) inhibitor with an IC50 of 1 μM.

In vitro activity:

NIH-12848 (10 μM) was applied to cultured mouse principal kidney cortical collecting duct (mpkCCD) cells which, we show, express PI5P4Kγ that increases when the cells grow to confluence and polarize. NIH-12848 inhibited the translocation of Na⁺/K⁺-ATPase to the plasma membrane that occurs when mpkCCD cells grow to confluence and also prevented reversibly their forming of 'domes' on the culture dish. Reference: Biochem J. 2015 Mar 1;466(2):359-67. https://pubmed.ncbi.nlm.nih.gov/25495341/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMSO	67.3	174.71
	Ethanol	77.0	200.00

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 385.41 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

Clarke JH, Giudici ML, Burke JE, Williams RL, Maloney DJ, Marugan J, Irvine RF. The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) explored using a specific inhibitor that targets the PI5P-binding site. Biochem J. 2015 Mar 1;466(2):359-67. doi: 10.1042/BJ20141333. PMID: 25495341; PMCID: PMC4687057.

In vitro protocol:

In vivo protocol:

TBD

1: Clarke JH, Giudici ML, Burke JE, Williams RL, Maloney DJ, Marugan J, Irvine RF. The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) explored using a specific inhibitor that targets the PI5P-binding site. Biochem J. 2015 Mar 1;466(2):359-67. doi: 10.1042/BJ20141333. PubMed PMID: 25495341; PubMed Central PMCID: PMC4687057.