COTI-2 is an P53 gene modulator and AKT2 inhibitor. COTI-2 is a third-generation thiosemicarbazone with selective anticancer activity, particularly in TP53-mutant tumors. In vitro studies have demonstrated that COTI-2 exhibits potent cytotoxicity across various cancer cell lines, including ovarian, head and neck, and colorectal cancers, with IC₅₀ values in the low micromolar to nanomolar range. Mechanistically, it restores mutant p53 to a wild-type conformation, leading to reactivation of p53-dependent apoptosis and cell cycle arrest. Additionally, COTI-2 inhibits the PI3K/AKT/mTOR pathway, contributing to its antiproliferative effects. These dual mechanisms of action make COTI-2 a promising candidate for targeted cancer therapy.
MedKoo Cat#: 206943
Name: COTI-2
CAS#: 1039455-84-9 (free base)
Chemical Formula: C19H22N6S
Exact Mass: 366.1627
Molecular Weight: 366.48
Elemental Analysis: C, 62.27; H, 6.05; N, 22.93; S, 8.75
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 3.0 | 8.19 |
The following data is based on the product molecular weight 366.48 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
