VID-400 (also known as SDZ285428) is a potent and selective inhibitor of sterol 14α-demethylase (CYP51), a critical enzyme in the ergosterol biosynthesis pathway of protozoan parasites such as Trypanosoma cruzi and T. brucei. It demonstrates strong in vitro activity, with rapid and sustained enzyme inhibition (I/E₂ < 1 at 5 min; I/E₂ = 9–35 at 60 min), making it a valuable tool for studying antiparasitic mechanisms targeting CYP51. NVP-VID-400 targets CYP51, a cytochrome P450 enzyme critical for the demethylation of lanosterol, a key step in ergosterol biosynthesis. By inhibiting CYP51, NVP-VID-400 disrupts the production of ergosterol, compromising the integrity of the parasite's cell membrane and leading to cell death.
MedKoo Cat#: 530360
Name: NVP-VID-400
CAS#: 174262-13-6
Chemical Formula: C24H20ClN3O
Exact Mass: 401.1295
Molecular Weight: 401.89
Elemental Analysis: C, 71.73; H, 5.02; Cl, 8.82; N, 10.46; O, 3.98
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.5 | 80.87 |
The following data is based on the product molecular weight 401.89 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
