ID-1101 |CAS#55399-93-4 | insulin sensitizer | obesity | type 2 diabetes

MedKoo Cat#: 530101 | Name: ID-1101

Description:

WARNING: This product is for research use only, not for human or veterinary use.

ID-1101, also known as Hydroxyisoleucine, is an insulin sensitizer potentially for the treatment of obesity and type 2 diabetes. It is an amino acid extracted from fenugreek seeds, and exhibits an interesting glucose-dependent insulin-stimulating activity, as shown from studies done on rats in vivo.

Chemical Structure

ID-1101

CAS#55399-93-4

Theoretical Analysis

MedKoo Cat#: 530101

Name: ID-1101

CAS#: 55399-93-4

Chemical Formula: C6H13NO3

Exact Mass: 147.0895

Molecular Weight: 147.17

Elemental Analysis: C, 48.97; H, 8.90; N, 9.52; O, 32.61

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Synonym

ID-1101; 4-OH-Ile; 4OHIle; ID 1101; 4 OH Ile; ID1101

IUPAC/Chemical Name

(2S,3R,4S)-4-Hydroxyisoleucine

InChi Key

OSCCDBFHNMXNME-YUPRTTJUSA-N

InChi Code

InChI=1S/C6H13NO3/c1-3(4(2)8)5(7)6(9)10/h3-5,8H,7H2,1-2H3,(H,9,10)/t3-,4-,5-/m0/s1

SMILES Code

N[C@@H]([C@@H](C)[C@@H](O)C)C(O)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

(2S,3R,4S)-4-Hydroxyisoleucine is an orally active compound isolated from Trigonella foenum-graecum, with anti-diabetes and anti-diabetic nephropathy activity.

In vitro activity:

In this study, ELISA was used to determine the effects of 4-HIL (ID-1101) on the levels of TNF-α and MCP-1 (Fig. 4). After 6 h of LPS stimulation, the levels of TNF-α and MCP-1 were reduced by 4-HIL in a dose-dependent (Fig. 4a and d) and time-dependent manner (Fig. 4b and e). Reference: BMC Complement Med Ther. 2020 Dec 9;20(1):373. https://pubmed.ncbi.nlm.nih.gov/33298044/

In vivo activity:

Chronic oral administration of 4-HI (ID-1101) at the doses of 50 mg/kg and 100 mg/kg remarkably increased the IGF-1 level in brain homogenate (one-way ANOVA: F(5, 25) = 5.595, p < 0.001) and CSF samples (one-way ANOVA: F(5,25) = 0.971, p < 0.001). Moreover, 4-HI100 mg/kg was more efficient than 4-HI50 mg/kg in restoring the IGF-1 protein level in rat brain homogenate and CSF samples (Figure 10A,B). Reference: Molecules. 2022 Jun 16;27(12):3878. https://pubmed.ncbi.nlm.nih.gov/35745001/

	Solvent	mg/mL	mM
Solubility
	PBS (pH 7.2)	5.0	33.97
	Water	125.0	849.33

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 147.17 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Zhou C, Chen R, Gao F, Zhang J, Lu F. 4-Hydroxyisoleucine relieves inflammation through iRhom2-dependent pathway in co-cultured macrophages and adipocytes with LPS stimulation. BMC Complement Med Ther. 2020 Dec 9;20(1):373. doi: 10.1186/s12906-020-03166-1. PMID: 33298044; PMCID: PMC7724822. 2. Gao F, Du W, Zafar MI, Shafqat RA, Jian L, Cai Q, Lu F. 4-Hydroxyisoleucine ameliorates an insulin resistant-like state in 3T3-L1 adipocytes by regulating TACE/TIMP3 expression. Drug Des Devel Ther. 2015 Oct 20;9:5727-36. doi: 10.2147/DDDT.S92355. PMID: 26527864; PMCID: PMC4621195. 3. Shandilya A, Mehan S, Kumar S, Sethi P, Narula AS, Alshammari A, Alharbi M, Alasmari AF. Activation of IGF-1/GLP-1 Signalling via 4-Hydroxyisoleucine Prevents Motor Neuron Impairments in Experimental ALS-Rats Exposed to Methylmercury-Induced Neurotoxicity. Molecules. 2022 Jun 16;27(12):3878. doi: 10.3390/molecules27123878. PMID: 35745001; PMCID: PMC9228431. 4. Mehmood Siddiqui E, Mehan S, Upadhayay S, Khan A, Halawi M, Ahmed Halawi A, Alsaffar RM. Neuroprotective efficacy of 4-Hydroxyisoleucine in experimentally induced intracerebral hemorrhage. Saudi J Biol Sci. 2021 Nov;28(11):6417-6431. doi: 10.1016/j.sjbs.2021.07.010. Epub 2021 Jul 10. PMID: 34764759; PMCID: PMC8568986.

In vitro protocol:

In vivo protocol:

1. Shandilya A, Mehan S, Kumar S, Sethi P, Narula AS, Alshammari A, Alharbi M, Alasmari AF. Activation of IGF-1/GLP-1 Signalling via 4-Hydroxyisoleucine Prevents Motor Neuron Impairments in Experimental ALS-Rats Exposed to Methylmercury-Induced Neurotoxicity. Molecules. 2022 Jun 16;27(12):3878. doi: 10.3390/molecules27123878. PMID: 35745001; PMCID: PMC9228431. 2. Mehmood Siddiqui E, Mehan S, Upadhayay S, Khan A, Halawi M, Ahmed Halawi A, Alsaffar RM. Neuroprotective efficacy of 4-Hydroxyisoleucine in experimentally induced intracerebral hemorrhage. Saudi J Biol Sci. 2021 Nov;28(11):6417-6431. doi: 10.1016/j.sjbs.2021.07.010. Epub 2021 Jul 10. PMID: 34764759; PMCID: PMC8568986.

1: Broca C, Breil V, Cruciani-Guglielmacci C, Manteghetti M, Rouault C, Derouet M, Rizkalla S, Pau B, Petit P, Ribes G, Ktorza A, Gross R, Reach G, Taouis M. Insulinotropic agent ID-1101 (4-hydroxyisoleucine) activates insulin signaling in rat. Am J Physiol Endocrinol Metab. 2004 Sep;287(3):E463-71. PubMed PMID: 15082420.