RS-1 | RAD51-Stimulatory Compound-1 | CAS#312756-74-4

MedKoo Cat#: 406955 | Name: RS-1

Description:

WARNING: This product is for research use only, not for human or veterinary use.

RS-1, also known as RAD51-Stimulatory Compound-1, a RAD51 activator with potential anticancer activity. RAD51 is the central protein that catalyzes DNA repair via homologous recombination, a process that ensures genomic stability. RAD51 protein is commonly expressed at high levels in cancer cells relative to their noncancerous precursors. High levels of RAD51 expression can lead to the formation of genotoxic RAD51 protein complexes on undamaged chromatin.

Chemical Structure

RS-1

CAS#312756-74-4

Theoretical Analysis

MedKoo Cat#: 406955

Name: RS-1

CAS#: 312756-74-4

Chemical Formula: C20H16Br2N2O3S

Exact Mass: 521.9248

Molecular Weight: 524.23

Elemental Analysis: C, 45.82; H, 3.08; Br, 30.48; N, 5.34; O, 9.16; S, 6.12

Price and Availability

Size	Price	Availability
100mg	USD 650.00	2 Weeks
200mg	USD 950.00	2 Weeks
500mg	USD 1,450.00	2 Weeks
1g	USD 2,250.00	2 Weeks
2g	USD 3,850.00	2 Weeks

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Synonym

RS-1; RS 1; RS1. RAD51-Stimulatory Compound-1.

IUPAC/Chemical Name

4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]benzamide

InChi Key

SWKAVEUTKGKHSR-UHFFFAOYSA-N

InChi Code

InChI=1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)

SMILES Code

O=C(NC1=CC=C(Br)C=C1)C2=CC=C(Br)C(S(=O)(NCC3=CC=CC=C3)=O)=C2

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO and DMF

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

RS-1 is an activator of DNA repair protein RAD51 (Kd = 48-107 for human RAD51 with different cofactors present).

In vitro activity:

RS-1 can increase both protein-DNA complex lengths and the pitch of helical filament turns. RS-1 stimulated hRAD51-mediated homologous strand assimilation (D-loop) activity by at least 5- to 11-fold, depending on the condition. RS-1 enhances the homologous recombination activity of hRAD51 by promoting the formation of active presynaptic filaments. RS-1 can enhance filament stability. Reference: Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15848-53. https://pubmed.ncbi.nlm.nih.gov/18840682/

In vivo activity:

This study found that the RS-1 significantly increased knock-in efficiency in vitro, with up to a five-fold improvement at different sites. In animal production, RS-1 yielded substantial improvements. This study provides insights for in vivo nuclease-mediated knock-in applications, enhancing gene-targeting efficiencies in pluripotent stem cells. Reference: Nat Commun. 2016 Jan 28;7:10548. https://pubmed.ncbi.nlm.nih.gov/26817820/

	Solvent	mg/mL	mM
Solubility
	DMF	30.0	57.23
	DMSO	20.0	38.15

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 524.23 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Jayathilaka K, Sheridan SD, Bold TD, Bochenska K, Logan HL, Weichselbaum RR, Bishop DK, Connell PP. A chemical compound that stimulates the human homologous recombination protein RAD51. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15848-53. doi: 10.1073/pnas.0808046105. Epub 2008 Oct 7. PMID: 18840682; PMCID: PMC2572930. 2. Bi R, Li Y, Xu M, Zheng Q, Zhang DF, Li X, Ma G, Xiang B, Zhu X, Zhao H, Huang X, Zheng P, Yao YG. Direct evidence of CRISPR-Cas9-mediated mitochondrial genome editing. Innovation (Camb). 2022 Sep 27;3(6):100329. doi: 10.1016/j.xinn.2022.100329. PMID: 36275864; PMCID: PMC9579715. 3. Song J, Yang D, Xu J, Zhu T, Chen YE, Zhang J. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat Commun. 2016 Jan 28;7:10548. doi: 10.1038/ncomms10548. PMID: 26817820; PMCID: PMC4738357.

In vitro protocol:

In vivo protocol:

1. Song J, Yang D, Xu J, Zhu T, Chen YE, Zhang J. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat Commun. 2016 Jan 28;7:10548. doi: 10.1038/ncomms10548. PMID: 26817820; PMCID: PMC4738357.

1: Mason JM, Logan HL, Budke B, Wu M, Pawlowski M, Weichselbaum RR, Kozikowski AP, Bishop DK, Connell PP. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors. Cancer Res. 2014 Jul 1;74(13):3546-55. doi: 10.1158/0008-5472.CAN-13-3220. PubMed PMID: 24753542; PubMed Central PMCID: PMC4079730.