MedKoo Cat#: 526871 | Name: GW-7647
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

GW7647 is a potent, selective PPARα agonist with an EC₅₀ of 6 nM and over 180-fold selectivity over PPARγ and PPARδ. It enhances mitochondrial fatty acid β-oxidation, reduces glycolysis, and significantly lowers triglycerides—up to 93% in hamsters and 60% in rats at 3 mg/kg. GW7647 also shows anti-inflammatory activity and alleviates acidosis-induced pain via PPARα-mediated inhibition of acid-sensing ion channels in sensory neurons.

Chemical Structure

GW-7647
GW-7647
CAS#265129-71-3

Theoretical Analysis

MedKoo Cat#: 526871

Name: GW-7647

CAS#: 265129-71-3

Chemical Formula: C29H46N2O3S

Exact Mass: 502.3229

Molecular Weight: 502.76

Elemental Analysis: C, 69.28; H, 9.22; N, 5.57; O, 9.55; S, 6.38

Price and Availability

Size Price Availability Quantity
5mg USD 90.00 Ready to ship
10mg USD 150.00 Ready to ship
25mg USD 250.00 Ready to ship
50mg USD 450.00 Ready to ship
100mg USD 750.00 Ready to ship
200mg USD 1,250.00 Ready to ship
500mg USD 2,650.00 Ready to ship
1g USD 3,850.00 Ready to ship
2g USD 6,450.00 Ready to ship
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Related CAS #
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Synonym
GW-7647; GW 7647; GW7647.
IUPAC/Chemical Name
2-((4-(2-(3-cyclohexyl-1-(4-cyclohexylbutyl)ureido)ethyl)phenyl)thio)-2-methylpropanoic acid
InChi Key
PKNYXWMTHFMHKD-UHFFFAOYSA-N
InChi Code
InChI=1S/C29H46N2O3S/c1-29(2,27(32)33)35-26-18-16-24(17-19-26)20-22-31(28(34)30-25-14-7-4-8-15-25)21-10-9-13-23-11-5-3-6-12-23/h16-19,23,25H,3-15,20-22H2,1-2H3,(H,30,34)(H,32,33)
SMILES Code
CC(C)(SC1=CC=C(CCN(C(NC2CCCCC2)=O)CCCCC3CCCCC3)C=C1)C(O)=O
Appearance
White to off-white solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Biological target:
GW7647 is a PPARα agonist, with EC50s of 6 nM, 1.1 μM, and 6.2 μM for human PPARα, PPARγ and PPARδ, respectively.
In vitro activity:
mRNA from primary human hepatocytes isolated from four independent donors was collected at five time points (2, 6, 12, 24, and 72 h) from cells treated with five concentrations of GW7647 between 0.001 and 10 μm. Only 192 genes were statistically significantly up- or down-regulated upon treatment of GW7647 at any of the dose or time points, with more than 80% showing up-regulation (Fig. 2B). These up-regulated genes encompassed many lipid metabolism pathway genes that are known targets of PPARα, including ACOX1, CPT1A, and APOA4. The differentially expressed genes we identified correlate strongly with the set of genes regulated by another PPARα-selective agonist, Wy14643 (Figure S1). Reference: Chem Biol Interact. 2014 Feb 25;209:14-24. https://pubmed.ncbi.nlm.nih.gov/24269660/
In vivo activity:
This study’s electrophysiological and behavioral evidence demonstrated that PPAR-α activation can acutely inhibit the activity of ASICs in nociceptive DRG neurons. PPAR-α agonist GW7647 decreased the amplitude of proton-activated currents and acidosis-triggered action potentials in rat isolated DRG neurons. Peripheral administration of GW7647 relieved acidosis-evoked nociceptive responses and CFA-induced mechanical hypersensitivity in rats. Reference: Oncotarget. 2017 Nov 3; 8(54): 93051–93062. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696243/
Solvent mg/mL mM
Solubility
DMSO 16.0 31.80
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 502.76 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Patil R, Mohanty B, Liu B, Chandrashekaran IR, Headey SJ, Williams ML, Clements CS, Ilyichova O, Doak BC, Genissel P, Weaver RJ, Vuillard L, Halls ML, Porter CJH, Scanlon MJ. A ligand-induced structural change in fatty acid-binding protein 1 is associated with potentiation of peroxisome proliferator-activated receptor α agonists. J Biol Chem. 2019 Mar 8;294(10):3720-3734. doi: 10.1074/jbc.RA118.006848. Epub 2018 Dec 31. PMID: 30598509; PMCID: PMC6416440. 2. McMullen PD, Bhattacharya S, Woods CG, Sun B, Yarborough K, Ross SM, Miller ME, McBride MT, LeCluyse EL, Clewell RA, Andersen ME. A map of the PPARα transcription regulatory network for primary human hepatocytes. Chem Biol Interact. 2014 Feb 25;209:14-24. doi: 10.1016/j.cbi.2013.11.006. Epub 2013 Nov 22. PMID: 24269660. 3. Wu J, Wang JJ, Liu TT, Zhou YM, Qiu CY, Shen DW, Hu WP. PPAR-α acutely inhibits functional activity of ASICs in rat dorsal root ganglion neurons. Oncotarget. 2017 Oct 10;8(54):93051-93062. doi: 10.18632/oncotarget.21805. PMID: 29190977; PMCID: PMC5696243. 4. Tanaka S, Hosogi S, Sawabe Y, Shimamoto C, Matsumura H, Inui T, Marunaka Y, Nakahari T. PPARα induced NOS1 phosphorylation via PI3K/Akt in guinea pig antral mucous cells: NO-enhancement in Ca(2+)-regulated exocytosis. Biomed Res. 2016;37(3):167-78. doi: 10.2220/biomedres.37.167. PMID: 27356604.
In vitro protocol:
1. Patil R, Mohanty B, Liu B, Chandrashekaran IR, Headey SJ, Williams ML, Clements CS, Ilyichova O, Doak BC, Genissel P, Weaver RJ, Vuillard L, Halls ML, Porter CJH, Scanlon MJ. A ligand-induced structural change in fatty acid-binding protein 1 is associated with potentiation of peroxisome proliferator-activated receptor α agonists. J Biol Chem. 2019 Mar 8;294(10):3720-3734. doi: 10.1074/jbc.RA118.006848. Epub 2018 Dec 31. PMID: 30598509; PMCID: PMC6416440. 2. McMullen PD, Bhattacharya S, Woods CG, Sun B, Yarborough K, Ross SM, Miller ME, McBride MT, LeCluyse EL, Clewell RA, Andersen ME. A map of the PPARα transcription regulatory network for primary human hepatocytes. Chem Biol Interact. 2014 Feb 25;209:14-24. doi: 10.1016/j.cbi.2013.11.006. Epub 2013 Nov 22. PMID: 24269660.
In vivo protocol:
1. Wu J, Wang JJ, Liu TT, Zhou YM, Qiu CY, Shen DW, Hu WP. PPAR-α acutely inhibits functional activity of ASICs in rat dorsal root ganglion neurons. Oncotarget. 2017 Oct 10;8(54):93051-93062. doi: 10.18632/oncotarget.21805. PMID: 29190977; PMCID: PMC5696243. 2. Tanaka S, Hosogi S, Sawabe Y, Shimamoto C, Matsumura H, Inui T, Marunaka Y, Nakahari T. PPARα induced NOS1 phosphorylation via PI3K/Akt in guinea pig antral mucous cells: NO-enhancement in Ca(2+)-regulated exocytosis. Biomed Res. 2016;37(3):167-78. doi: 10.2220/biomedres.37.167. PMID: 27356604.
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