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MedKoo Cat#: 317327 | Name: Biotin
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.

Chemical Structure

Biotin
Biotin
CAS#58-85-5

Theoretical Analysis

MedKoo Cat#: 317327

Name: Biotin

CAS#: 58-85-5

Chemical Formula: C10H16N2O3S

Exact Mass: 244.0882

Molecular Weight: 244.31

Elemental Analysis: C, 49.16; H, 6.60; N, 11.47; O, 19.65; S, 13.12

Price and Availability

Size Price Availability Quantity
5g USD 350.00 2 Weeks
10g USD 550.00 2 Weeks
25g USD 950.00 2 Weeks
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Related CAS #
No Data
Synonym
Biotin; D-Biotin; Vitamin B7; Vitamin H
IUPAC/Chemical Name
5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid
InChi Key
YBJHBAHKTGYVGT-UHFFFAOYSA-N
InChi Code
InChI=1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)
SMILES Code
C1C2C(C(S1)CCCCC(=O)O)NC(=O)N2
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 244.31 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Coffey CM, Gronert S. A cleavable biotin tagging reagent that enables the enrichment and identification of carbonylation sites in proteins. Anal Bioanal Chem. 2015 Nov 27. [Epub ahead of print] PubMed PMID: 26613796. 2: Järvinen E, Ismail K, Muniandy M, Bogl L, Heinonen S, Tummers M, Miettinen S, Kaprio J, Rissanen A, Ollikainen M, Pietiläinen K. Biotin dependent functions in adiposity-a study of monozygotic twin pairs. Int J Obes (Lond). 2015 Nov 25. doi: 10.1038/ijo.2015.237. [Epub ahead of print] PubMed PMID: 26601567. 3: Etemadzadeh MH, Arashkia A, Roohvand F, Ahani R, Mohajel N, Baniasadi V, Norouzian D, Azadmanesh K. Expression of a biotin acceptor peptide-containing protein with potential incorporation on the lentiviral envelope as a viral surface engineering platform. Res Pharm Sci. 2015 Jul-Aug;10(4):268-274. PubMed PMID: 26600854; PubMed Central PMCID: PMC4623616. 4: Wu J, Luo X, Jing S, Yan LJ. Two-dimensional gel electrophoretic detection of protein carbonyls derivatized with biotin-hydrazide. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Nov 7. pii: S1570-0232(15)30268-3. doi: 10.1016/j.jchromb.2015.11.003. [Epub ahead of print] PubMed PMID: 26590475. 5: Kinoshita-Kikuta E, Kinoshita E, Koike T. Phosphopeptide Detection with Biotin-Labeled Phos-tag. Methods Mol Biol. 2016;1355:17-29. doi: 10.1007/978-1-4939-3049-4_2. PubMed PMID: 26584916. 6: Xia B, Pang L, Zhuang ZX, Liu JJ. Biotin-mediated epigenetic modifications: Potential defense against the carcinogenicity of benzo[a]pyrene. Toxicol Lett. 2015 Nov 10. pii: S0378-4274(15)30107-7. doi: 10.1016/j.toxlet.2015.11.010. [Epub ahead of print] PubMed PMID: 26569572. 7: Krishna KV, Ghosh S, Sharma B, Singh L, Mukherjee S, Verma S. Fluorescent Biotin Analogues for Microstructure Patterning and Selective Protein Immobilization. Langmuir. 2015 Nov 24;31(46):12573-8. doi: 10.1021/acs.langmuir.5b03476. Epub 2015 Nov 13. PubMed PMID: 26559028. 8: Teramura Y, Kuroyama K, Takai M. Influence of molecular weight of PEG chain on interaction between streptavidin and biotin-PEG-conjugated phospholipids studied with QCM-D. Acta Biomater. 2015 Nov 3. pii: S1742-7061(15)30180-X. doi: 10.1016/j.actbio.2015.11.003. [Epub ahead of print] PubMed PMID: 26546413. 9: Bong KW, Kim JJ, Cho H, Lim E, Doyle PS, Irimia D. Synthesis of Cell-Adhesive Anisotropic Multifunctional Particles by Stop Flow Lithography and Streptavidin-Biotin Interactions. Langmuir. 2015 Nov 25. [Epub ahead of print] PubMed PMID: 26545155. 10: Soural M, Hodon J, Dickinson NJ, Sidova V, Gurska S, Dzubak P, Hajduch M, Sarek J, Urban M. Preparation of Conjugates of Cytotoxic Lupane Triterpenes with Biotin. Bioconjug Chem. 2015 Nov 12. [Epub ahead of print] PubMed PMID: 26537839. 11: Kang CM, Koo HJ, An GI, Choe YS, Choi JY, Lee KH, Kim BT. Hybrid PET/optical imaging of integrin αVβ3 receptor expression using a (64)Cu-labeled streptavidin/biotin-based dimeric RGD peptide. EJNMMI Res. 2015 Dec;5(1):60. doi: 10.1186/s13550-015-0140-0. Epub 2015 Oct 31. PubMed PMID: 26518424; PubMed Central PMCID: PMC4628001. 12: Jordán-Pla A, Miguel A, Serna E, Pelechano V, Pérez-Ortín JE. Biotin-Genomic Run-On (Bio-GRO): A High-Resolution Method for the Analysis of Nascent Transcription in Yeast. Methods Mol Biol. 2016;1361:125-39. doi: 10.1007/978-1-4939-3079-1_8. PubMed PMID: 26483020. 13: Kajiwara H, Tsunashima M, Mine T, Takakura Y, Yamamoto T. Immobilized sialyltransferase fused to a fungal biotin-binding protein: Production, properties, and applications. J Biosci Bioeng. 2015 Oct 14. pii: S1389-1723(15)00331-X. doi: 10.1016/j.jbiosc.2015.08.016. [Epub ahead of print] PubMed PMID: 26476897. 14: Hersch N, Wolters B, Ungvari Z, Gautam T, Deshpande D, Merkel R, Csiszar A, Hoffmann B, Csiszár A. Biotin-conjugated fusogenic liposomes for high-quality cell purification. J Biomater Appl. 2015 Oct 15. pii: 0885328215603026. [Epub ahead of print] PubMed PMID: 26475851. 15: Tan SM, Lieberman J. Capture and Identification of miRNA Targets by Biotin Pulldown and RNA-seq. Methods Mol Biol. 2016;1358:211-28. doi: 10.1007/978-1-4939-3067-8_13. PubMed PMID: 26463386. 16: Singh S, Tiwari AK, Varshney R, Mathur R, Shukla G, Bag N, Singh B, Mishra AK. Comparative evaluation of Bis(thiosemicarbazone)- Biotin and Met-ac-TE3A for tumor imaging. Spectrochim Acta A Mol Biomol Spectrosc. 2016 Jan 15;153:566-71. doi: 10.1016/j.saa.2015.08.054. Epub 2015 Sep 4. PubMed PMID: 26436844. 17: Shetty D, Khedkar JK, Park KM, Kim K. Can we beat the biotin-avidin pair?: cucurbit[7]uril-based ultrahigh affinity host-guest complexes and their applications. Chem Soc Rev. 2015 Dec 7;44(23):8747-61. doi: 10.1039/c5cs00631g. Epub 2015 Oct 5. PubMed PMID: 26434388. 18: Uram Ł, Szuster M, Filipowicz A, Gargasz K, Wołowiec S, Wałajtys-Rode E. Different patterns of nuclear and mitochondrial penetration by the G3 PAMAM dendrimer and its biotin-pyridoxal bioconjugate BC-PAMAM in normal and cancer cells in vitro. Int J Nanomedicine. 2015 Sep 4;10:5647-61. doi: 10.2147/IJN.S87307. eCollection 2015. PubMed PMID: 26379435; PubMed Central PMCID: PMC4567239. 19: Rana A, Dey S, Agrawal A, Dey A. Density functional theory calculations on the active site of biotin synthase: mechanism of S transfer from the Fe2S2 cluster and the role of 1st and 2nd sphere residues. J Biol Inorg Chem. 2015 Oct;20(7):1147-62. doi: 10.1007/s00775-015-1296-9. Epub 2015 Sep 14. PubMed PMID: 26369537. 20: Ochoa-Ruiz E, Díaz-Ruiz R, Hernández-Vázquez Ade J, Ibarra-González I, Ortiz-Plata A, Rembao D, Ortega-Cuéllar D, Viollet B, Uribe-Carvajal S, Corella JA, Velázquez-Arellano A. Biotin deprivation impairs mitochondrial structure and function and has implications for inherited metabolic disorders. Mol Genet Metab. 2015 Nov;116(3):204-14. doi: 10.1016/j.ymgme.2015.08.009. Epub 2015 Sep 1. PubMed PMID: 26343941.