SANT-1 | CAS#304909-07-7 | Sonic hedgehog (Shh) enzyme inhibitor

MedKoo Cat#: 407193 | Name: SANT-1

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

SANT-1 is a potent and selective Sonic hedgehog (Shh) enzyme inhibitor with potential anticancer activities. Sonic hedgehog (Shh) is expressed in AML biopsies and is essential for the drug resistance of cancer stem cells of AML.

Chemical Structure

SANT-1

CAS#304909-07-7

Theoretical Analysis

MedKoo Cat#: 407193

Name: SANT-1

CAS#: 304909-07-7

Chemical Formula: C23H27N5

Exact Mass: 373.2267

Molecular Weight: 373.50

Elemental Analysis: C, 73.96; H, 7.29; N, 18.75

Price and Availability

Size	Price	Availability
5mg	USD 375.00	2 weeks
10mg	USD 550.00	2 weeks
50mg	USD 950.00	2 weeks
100mg	USD 1,350.00	2 weeks

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Related CAS #

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Synonym

SANT-1; SANT 1; SANT1.

IUPAC/Chemical Name

N-[(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methylene]-4-(phenylmethyl)-1-piperazinamine

InChi Key

FOORCIAZMIWALX-JJIBRWJFSA-N

InChi Code

InChI=1S/C23H27N5/c1-19-23(20(2)28(25-19)22-11-7-4-8-12-22)17-24-27-15-13-26(14-16-27)18-21-9-5-3-6-10-21/h3-12,17H,13-16,18H2,1-2H3/b24-17+

SMILES Code

CC1=C(/C=N/N2CCN(CC3=CC=CC=C3)CC2)C(C)=NN1C4=CC=CC=C4

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO and ethanol

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

SANT-1 directly binds to Smoothened (Smo) receptor with Kd of 1.2 nM and inhibits Smo agonist effects with IC50 of 20 nM.

In vitro activity:

Cyclopamine and jervine, two structurally related inhibitors of Smo, force ciliary translocation of Smo. Treatment with SANT-1 abrogates cyclopamine- and jervine-mediated Smo translocation. Reference: PLoS One. 2009;4(4):e5182. https://pubmed.ncbi.nlm.nih.gov/19365551/

In vivo activity:

To be determined

	Solvent	mg/mL	mM
Solubility
	DMSO	21.0	56.23
	Ethanol	20.0	53.55

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 373.50 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Dixit D, Ghildiyal R, Anto NP, Ghosh S, Sharma V, Sen E. Guggulsterone sensitizes glioblastoma cells to Sonic hedgehog inhibitor SANT-1 induced apoptosis in a Ras/NFκB dependent manner. Cancer Lett. 2013 Aug 19;336(2):347-58. doi: 10.1016/j.canlet.2013.03.025. Epub 2013 Mar 31. PMID: 23548480. 2. Wilson CW, Chen MH, Chuang PT. Smoothened adopts multiple active and inactive conformations capable of trafficking to the primary cilium. PLoS One. 2009;4(4):e5182. doi: 10.1371/journal.pone.0005182. Epub 2009 Apr 13. PMID: 19365551; PMCID: PMC2664476.

In vitro protocol:

In vivo protocol:

To be determined