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MedKoo Cat#: 407169 | Name: STF31
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

STF31 is a potent glucose transporter 1 (GLUT1). STF31 selectively kills RCCs by specifically targeting glucose uptake through GLUT1 and exploiting the unique dependence of these cells on GLUT1 for survival. Treatment with STF31 inhibits the growth of RCCs by binding GLUT1 directly and impeding glucose uptake in vivo without toxicity to normal tissue. STF-31 is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures.

Chemical Structure

STF31
STF31
CAS#724741-75-7

Theoretical Analysis

MedKoo Cat#: 407169

Name: STF31

CAS#: 724741-75-7

Chemical Formula: C23H25N3O3S

Exact Mass: 423.1617

Molecular Weight: 423.53

Elemental Analysis: C, 65.23; H, 5.95; N, 9.92; O, 11.33; S, 7.57

Price and Availability

Size Price Availability Quantity
25mg USD 110.00 Ready to ship
50mg USD 190.00 Ready to ship
100mg USD 350.00 Ready to ship
200mg USD 650.00 Ready to ship
500mg USD 1,450.00 Ready to ship
1g USD 2,450.00 Ready to ship
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Related CAS #
No Data
Synonym
STF31; STF-31; STF 31.
IUPAC/Chemical Name
4-(((4-(tert-butyl)phenyl)sulfonamido)methyl)-N-(pyridin-3-yl)benzamide
InChi Key
NGQPRVWTFNBUHA-UHFFFAOYSA-N
InChi Code
InChI=1S/C23H25N3O3S/c1-23(2,3)19-10-12-21(13-11-19)30(28,29)25-15-17-6-8-18(9-7-17)22(27)26-20-5-4-14-24-16-20/h4-14,16,25H,15H2,1-3H3,(H,26,27)
SMILES Code
O=C(NC1=CC=CN=C1)C2=CC=C(CNS(=O)(C3=CC=C(C(C)(C)C)C=C3)=O)C=C2
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Biological target:
STF-31 is a selective inhibitor of glucose transporter 1 (GLUT1), with an IC50 of 1μM.
In vitro activity:
STF31 targets NAMPT, a key enzyme in the conversion of nicotinamide to NAD+ in the NAD+ salvage pathway. The toxicity of STF31 in hPSCs is linked to NAD+ depletion rather than glucose transport inhibition. Reference: Stem Cells Transl Med. 2015 May; 4(5): 483–493. https://pubmed.ncbi.nlm.nih.gov/25834119/
In vivo activity:
In normal mice, injecting STF31 did not affect their body weight, behavior, or ERG responses and did not cause retinal cell death. After exposure to light, there was significant photoreceptor loss and microglial activation. However, STF31 treatment improved photoreceptor survival and reduced microglial activation compared to untreated mice. Examination showed improvements in retinal structure and thickness, particularly in the outer retinal layers, with STF31 treatment, but not in inner retinal thickness. Reference: Mol Neurodegener. 2019 Jan 11;14(1):2. https://pubmed.ncbi.nlm.nih.gov/30634998/
Solvent mg/mL mM
Solubility
DMSO 32.1 75.84
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 423.53 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Kropp EM, Oleson BJ, Broniowska KA, Bhattacharya S, Chadwick AC, Diers AR, Hu Q, Sahoo D, Hogg N, Boheler KR, Corbett JA, Gundry RL. Inhibition of an NAD⁺ salvage pathway provides efficient and selective toxicity to human pluripotent stem cells. Stem Cells Transl Med. 2015 May;4(5):483-93. doi: 10.5966/sctm.2014-0163. Epub 2015 Apr 1. PMID: 25834119; PMCID: PMC4414215. 2. Adams DJ, Ito D, Rees MG, Seashore-Ludlow B, Puyang X, Ramos AH, Cheah JH, Clemons PA, Warmuth M, Zhu P, Shamji AF, Schreiber SL. NAMPT is the cellular target of STF-31-like small-molecule probes. ACS Chem Biol. 2014 Oct 17;9(10):2247-54. doi: 10.1021/cb500347p. Epub 2014 Aug 7. PMID: 25058389; PMCID: PMC4201331. 3. Wang L, Pavlou S, Du X, Bhuckory M, Xu H, Chen M. Glucose transporter 1 critically controls microglial activation through facilitating glycolysis. Mol Neurodegener. 2019 Jan 11;14(1):2. doi: 10.1186/s13024-019-0305-9. PMID: 30634998; PMCID: PMC6329071. 4. Pingitore A, Ruz-Maldonado I, Liu B, Huang GC, Choudhary P, Persaud SJ. Dynamic Profiling of Insulin Secretion and ATP Generation in Isolated Human and Mouse Islets Reveals Differential Glucose Sensitivity. Cell Physiol Biochem. 2017;44(4):1352-1359. doi: 10.1159/000485532. Epub 2017 Nov 30. PMID: 29186709.
In vitro protocol:
1. Kropp EM, Oleson BJ, Broniowska KA, Bhattacharya S, Chadwick AC, Diers AR, Hu Q, Sahoo D, Hogg N, Boheler KR, Corbett JA, Gundry RL. Inhibition of an NAD⁺ salvage pathway provides efficient and selective toxicity to human pluripotent stem cells. Stem Cells Transl Med. 2015 May;4(5):483-93. doi: 10.5966/sctm.2014-0163. Epub 2015 Apr 1. PMID: 25834119; PMCID: PMC4414215. 2. Adams DJ, Ito D, Rees MG, Seashore-Ludlow B, Puyang X, Ramos AH, Cheah JH, Clemons PA, Warmuth M, Zhu P, Shamji AF, Schreiber SL. NAMPT is the cellular target of STF-31-like small-molecule probes. ACS Chem Biol. 2014 Oct 17;9(10):2247-54. doi: 10.1021/cb500347p. Epub 2014 Aug 7. PMID: 25058389; PMCID: PMC4201331.
In vivo protocol:
1. Wang L, Pavlou S, Du X, Bhuckory M, Xu H, Chen M. Glucose transporter 1 critically controls microglial activation through facilitating glycolysis. Mol Neurodegener. 2019 Jan 11;14(1):2. doi: 10.1186/s13024-019-0305-9. PMID: 30634998; PMCID: PMC6329071. 2. Pingitore A, Ruz-Maldonado I, Liu B, Huang GC, Choudhary P, Persaud SJ. Dynamic Profiling of Insulin Secretion and ATP Generation in Isolated Human and Mouse Islets Reveals Differential Glucose Sensitivity. Cell Physiol Biochem. 2017;44(4):1352-1359. doi: 10.1159/000485532. Epub 2017 Nov 30. PMID: 29186709.
1: Kuo CY, Hsu YC, Chen MJ, Lin CH, Li YS, Cheng SP. Glucose Transporter 1 Inhibitors Induce Autophagy and Synergize With Lenvatinib in Thyroid Cancer Cells. Head Neck. 2024 Oct 3. doi: 10.1002/hed.27953. Epub ahead of print. PMID: 39360406. 2: Zhou G, Lin L, Wang S, Dong M, Lu K, Zhang Y, Lin Z, Lin J, Wu W, Peng R, Luo C. Huanglian Jiedu Decoction enhances the stability of atherosclerotic plaques through SLC2A1-mediated efferocytosis. Int Immunopharmacol. 2024 Oct 25;140:112834. doi: 10.1016/j.intimp.2024.112834. Epub 2024 Aug 7. PMID: 39116495. 3: Abyar S, Huang L, Husiev Y, Bretin L, Chau B, Ramu V, Wildeman JH, Belfor K, Wijaya LS, van der Noord VE, Harms AC, Siegler MA, Le Dévédec SE, Bonnet S. Oxygen-Dependent Interactions between the Ruthenium Cage and the Photoreleased Inhibitor in NAMPT-Targeted Photoactivated Chemotherapy. J Med Chem. 2024 Jul 11;67(13):11086-11102. doi: 10.1021/acs.jmedchem.4c00589. Epub 2024 Jun 26. PMID: 38924492; PMCID: PMC11247496. 4: Luo P, Du M, Sun Q, Zhao T, He H. IL-38 suppresses macrophage M1 polarization to ameliorate synovial inflammation in the TMJ via GLUT-1 inhibition. Int Immunopharmacol. 2023 Sep;122:110619. doi: 10.1016/j.intimp.2023.110619. Epub 2023 Jul 17. PMID: 37463548. 5: Zhang ZD, Tao Q, Bai LX, Qin Z, Liu XW, Li SH, Yang YJ, Ge WB, Li JY. The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells. Molecules. 2023 Jun 6;28(12):4569. doi: 10.3390/molecules28124569. PMID: 37375124; PMCID: PMC10301264. 6: Winter J, Kunze R, Veit N, Kuerpig S, Meisenheimer M, Kraus D, Glassmann A, Probstmeier R. Targeting of Glucose Transport and the NAD Pathway in Neuroendocrine Tumor (NET) Cells Reveals New Treatment Options. Cancers (Basel). 2023 Feb 23;15(5):1415. doi: 10.3390/cancers15051415. PMID: 36900207; PMCID: PMC10001048. 7: Zou D, Yang P, Liu J, Dai F, Xiao Y, Zhao A, Huang N. Constructing Mal- Efferocytic Macrophage Model and Its Atherosclerotic Spheroids and Rat Model for Therapeutic Evaluation. Adv Biol (Weinh). 2023 Jun;7(6):e2200277. doi: 10.1002/adbi.202200277. Epub 2023 Jan 31. PMID: 36721069. 8: van Geest EP, Götzfried SK, Klein DM, Salitra N, Popal S, Husiev Y, Van der Griend CJ, Zhou X, Siegler MA, Schneider GF, Bonnet S. A Lock-and-Kill Anticancer Photoactivated Chemotherapy Agent†. Photochem Photobiol. 2023 Mar;99(2):777-786. doi: 10.1111/php.13738. Epub 2022 Dec 1. PMID: 36315051. 9: Cao X, Cao Y, Zhao H, Wang P, Zhu Z. Prolyl 4-hydroxylase P4HA1 Mediates the Interplay Between Glucose Metabolism and Stemness in Pancreatic Cancer Cells. Curr Stem Cell Res Ther. 2023;18(5):712-719. doi: 10.2174/1574888X17666220827113434. PMID: 36043766. 10: Krawczyk K, Marynowicz W, Gogola-Mruk J, Jakubowska K, Tworzydło W, Opydo- Chanek M, Ptak A. A mixture of persistent organic pollutants detected in human follicular fluid increases progesterone secretion and mitochondrial activity in human granulosa HGrC1 cells. Reprod Toxicol. 2021 Sep;104:114-124. doi: 10.1016/j.reprotox.2021.07.009. Epub 2021 Jul 24. PMID: 34311058. 11: Jiang A, Zhang Y, Wu D, Li S, Liu Z, Yang Z, Wei Z. Sodium molybdate induces heterophil extracellular traps formation in chicken. Ecotoxicol Environ Saf. 2021 Mar 1;210:111886. doi: 10.1016/j.ecoenv.2020.111886. Epub 2021 Jan 8. PMID: 33421725. 12: Ocaña MC, Martínez-Poveda B, Marí-Beffa M, Quesada AR, Medina MÁ. Fasentin diminishes endothelial cell proliferation, differentiation and invasion in a glucose metabolism-independent manner. Sci Rep. 2020 Apr 9;10(1):6132. doi: 10.1038/s41598-020-63232-z. PMID: 32273578; PMCID: PMC7145862. 13: Zhang T, Ouyang H, Mei X, Lu B, Yu Z, Chen K, Wang Z, Ji L. Erianin alleviates diabetic retinopathy by reducing retinal inflammation initiated by microglial cells via inhibiting hyperglycemia-mediated ERK1/2-NF-κB signaling pathway. FASEB J. 2019 Nov;33(11):11776-11790. doi: 10.1096/fj.201802614RRR. Epub 2019 Jul 31. PMID: 31365278; PMCID: PMC6902687. 14: Suzuki K, Miura Y, Mochida Y, Miyazaki T, Toh K, Anraku Y, Melo V, Liu X, Ishii T, Nagano O, Saya H, Cabral H, Kataoka K. Glucose transporter 1-mediated vascular translocation of nanomedicines enhances accumulation and efficacy in solid tumors. J Control Release. 2019 May 10;301:28-41. doi: 10.1016/j.jconrel.2019.02.021. Epub 2019 Mar 4. PMID: 30844476. 15: Wang L, Pavlou S, Du X, Bhuckory M, Xu H, Chen M. Glucose transporter 1 critically controls microglial activation through facilitating glycolysis. Mol Neurodegener. 2019 Jan 11;14(1):2. doi: 10.1186/s13024-019-0305-9. PMID: 30634998; PMCID: PMC6329071. 16: Kraus D, Reckenbeil J, Veit N, Kuerpig S, Meisenheimer M, Beier I, Stark H, Winter J, Probstmeier R. Targeting glucose transport and the NAD pathway in tumor cells with STF-31: a re-evaluation. Cell Oncol (Dordr). 2018 Oct;41(5):485-494. doi: 10.1007/s13402-018-0385-5. Epub 2018 Jun 11. PMID: 29949049. 17: Xintaropoulou C, Ward C, Wise A, Queckborner S, Turnbull A, Michie CO, Williams ARW, Rye T, Gourley C, Langdon SP. Expression of glycolytic enzymes in ovarian cancers and evaluation of the glycolytic pathway as a strategy for ovarian cancer treatment. BMC Cancer. 2018 Jun 5;18(1):636. doi: 10.1186/s12885-018-4521-4. PMID: 29866066; PMCID: PMC5987622. 18: Pingitore A, Ruz-Maldonado I, Liu B, Huang GC, Choudhary P, Persaud SJ. Dynamic Profiling of Insulin Secretion and ATP Generation in Isolated Human and Mouse Islets Reveals Differential Glucose Sensitivity. Cell Physiol Biochem. 2017;44(4):1352-1359. doi: 10.1159/000485532. Epub 2017 Nov 30. PMID: 29186709. 19: Nishimura R, Hasegawa H, Yamashita M, Ito N, Okamoto Y, Takeuchi T, Kubo T, Iga K, Kimura K, Hishinuma M, Okuda K. Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage. J Vet Med Sci. 2017 Nov 17;79(11):1878-1883. doi: 10.1292/jvms.17-0284. Epub 2017 Oct 17. PMID: 29046497; PMCID: PMC5709568. 20: Deck CA, Anderson WG, Conlon JM, Walsh PJ. The activity of the rectal gland of the North Pacific spiny dogfish Squalus suckleyi is glucose dependent and stimulated by glucagon-like peptide-1. J Comp Physiol B. 2017 Dec;187(8):1155-1161. doi: 10.1007/s00360-017-1102-9. Epub 2017 Apr 25. PMID: 28444441.