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MedKoo Cat#: 522385 | Name: CASIN
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

CASIN is an inhibitor of Cdc42 GTPase, a small GTPase of the Rho-subfamily that plays important roles in cytoskeleton organization, cell cycle progression, signal transduction, and vesicle trafficking. CASIN generated a rejuvenated phenotype of Hematopoietic stem cells (HSCs), reversing the aging-related and polarity phenotype of aged HSCs to that of young HSCs in vivo.

Chemical Structure

CASIN
CASIN
CAS#425399-05-9

Theoretical Analysis

MedKoo Cat#: 522385

Name: CASIN

CAS#: 425399-05-9

Chemical Formula: C20H22N2O

Exact Mass: 306.1732

Molecular Weight: 306.41

Elemental Analysis: C, 78.40; H, 7.24; N, 9.14; O, 5.22

Price and Availability

Size Price Availability Quantity
10mg USD 150.00 Ready to ship
25mg USD 250.00 Ready to ship
50mg USD 450.00 Ready to ship
100mg USD 750.00 Ready to ship
200mg USD 1,250.00 Ready to ship
500mg USD 2,650.00 Ready to ship
1g USD 3,950.00 Ready to ship
2g USD 6,450.00 2 Weeks
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Related CAS #
No Data
Synonym
CASIN; Pirl1-related Compound 2
IUPAC/Chemical Name
2-[(2,3,4,9-Tetrahydro-6-phenyl-1H-carbazol-1-yl)amino]ethanol
InChi Key
RXIUMAOXORBRCY-UHFFFAOYSA-N
InChi Code
InChI=1S/C20H22N2O/c23-12-11-21-19-8-4-7-16-17-13-15(14-5-2-1-3-6-14)9-10-18(17)22-20(16)19/h1-3,5-6,9-10,13,19,21-23H,4,7-8,11-12H2
SMILES Code
OCCNC1C(NC2=C3C=C(C4=CC=CC=C4)C=C2)=C3CCC1
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Biological target:
CASIN is a selective GTPase Cdc42 inhibitor with IC50 of 2 uM.
In vitro activity:
In addition, CASIN was studied to see how it affects cell migration, apoptosis and cell cycle of endothelial cells. CASIN upregulates key endothelial marker genes and downregulates a number of cyclin dependent kinase inhibitors. These findings suggest that CASIN could be further tested in the development of artificial vascular systems and vascular gene editing technologies. Furthermore, these findings may have potential to contribute to the development of alternative treatment methods for diseases that cause endothelial damage. Reference: Microvasc Res. 2020 Jul;130:104001. https://linkinghub.elsevier.com/retrieve/pii/S0026-2862(20)30061-3
In vivo activity:
It was demonstrated that systemic treatment of aged (75-week-old) female C57BL/6 mice with a Cdc42 activity-specific inhibitor (CASIN) for 4 consecutive days significantly extends average and maximum lifespan. Moreover, aged CASIN-treated animals displayed a youthful level of the aging-associated cytokines IL-1β, IL-1α, and INFγ in serum and a significantly younger epigenetic clock as based on DNA methylation levels in blood cells. Overall, our data show that systemic administration of CASIN to reduce Cdc42 activity in aged mice extends murine lifespan. Reference: Aging Cell. 2020 Sep;19(9):e13208. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32755011/
Solvent mg/mL mM
Solubility
DMSO 31.0 101.17
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 306.41 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
1. Kalkan BM, Akgol S, Ak D, Yucel D, Guney Esken G, Kocabas F. CASIN and AMD3100 enhance endothelial cell proliferation, tube formation and sprouting. Microvasc Res. 2020 Jul;130:104001. doi: 10.1016/j.mvr.2020.104001. Epub 2020 Mar 19. PMID: 32198058. 2. Leins H, Mulaw M, Eiwen K, Sakk V, Liang Y, Denkinger M, Geiger H, Schirmbeck R. Aged murine hematopoietic stem cells drive aging-associated immune remodeling. Blood. 2018 Aug 9;132(6):565-576. doi: 10.1182/blood-2018-02-831065. Epub 2018 Jun 11. PMID: 29891535; PMCID: PMC6137572.
In vivo protocol:
1. Florian MC, Leins H, Gobs M, Han Y, Marka G, Soller K, Vollmer A, Sakk V, Nattamai KJ, Rayes A, Zhao X, Setchell K, Mulaw M, Wagner W, Zheng Y, Geiger H. Inhibition of Cdc42 activity extends lifespan and decreases circulating inflammatory cytokines in aged female C57BL/6 mice. Aging Cell. 2020 Sep;19(9):e13208. doi: 10.1111/acel.13208. Epub 2020 Aug 4. PMID: 32755011; PMCID: PMC7511875. 2. Leins H, Mulaw M, Eiwen K, Sakk V, Liang Y, Denkinger M, Geiger H, Schirmbeck R. Aged murine hematopoietic stem cells drive aging-associated immune remodeling. Blood. 2018 Aug 9;132(6):565-576. doi: 10.1182/blood-2018-02-831065. Epub 2018 Jun 11. PMID: 29891535; PMCID: PMC6137572.