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MedKoo Cat#: 601122 | Name: Asiatic Acid
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Asiatic acid is a pentacyclic triterpene isolated from a variety of plants, including C. asiatica, used in traditional medicines. In addition to anti-inflammatory actions, asiatic acid stimulates wound healing by increasing collagen production. It has been found to induce cell cycle arrest and apoptosis in breast cancer cells and block angiogenesis in cells and tumors from glioblastomas. It also reduces neuronal damage and cognitive defects resulting from glutamate administration in vivo in mice while demonstrating protective effects against glutamate-induced apoptosis in isolated SH-SY5Y cells.

Chemical Structure

Asiatic Acid
Asiatic Acid
CAS#464-92-6

Theoretical Analysis

MedKoo Cat#: 601122

Name: Asiatic Acid

CAS#: 464-92-6

Chemical Formula: C30H48O5

Exact Mass: 488.3502

Molecular Weight: 488.70

Elemental Analysis: C, 73.73; H, 9.90; O, 16.37

Price and Availability

Size Price Availability Quantity
50mg USD 250.00 2 Weeks
100mg USD 400.00 2 Weeks
500mg USD 750.00 2 Weeks
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Related CAS #
No Data
Synonym
Dammarolic Acid; NSC 166063; NSC166063; NSC-166063
IUPAC/Chemical Name
(4α)-​2α,​3β,​23-​trihydroxy-​urs-​12-​en-​28-​oic acid
InChi Key
JXSVIVRDWWRQRT-UYDOISQJSA-N
InChi Code
InChI=1S/C30H48O5/c1-17-9-12-30(25(34)35)14-13-28(5)19(23(30)18(17)2)7-8-22-26(3)15-20(32)24(33)27(4,16-31)21(26)10-11-29(22,28)6/h7,17-18,20-24,31-33H,8-16H2,1-6H3,(H,34,35)/t17-,18+,20-,21-,22-,23+,24+,26+,27+,28-,29-,30+/m1/s1
SMILES Code
C[C@@]1(CO)[C@@H](O)[C@H](O)C[C@]2(C)[C@@]3([H])CC=C4[C@]5([H])[C@@H](C)[C@H](C)CC[C@@](C(O)=O)5CC[C@](C)4[C@@](C)3CC[C@@]12[H]
Appearance
Solid powder
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO.
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Asiatic acid, a pentacyclic triterpene found in Centella asiatica, induces apoptosis in melanoma cells.
In vitro activity:
This study began by assessing the impact of AA (Asiatic Acid) on tongue cancer cell viability via an MTT assay, which revealed that AA application significantly suppressed the viability of these cells with an IC50 value of approximately 40°μM (Figure 1A). Consistent with this, AA treatment (40°μM) significantly suppressed the colony forming activity of Tca8113 cells (Figure 1B). Reference: Front Pharmacol. 2021; 12: 690612. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187906/
In vivo activity:
AngII infusion rats showed a significant increase in the ratio of weight/tibia length (HW/TL), and this increase was attenuated in AA (Asiatic Acid)-treated rats (Fig. 2a). The thickness of the left ventricular post wall at the end-diastole (LVPWd) and the end-systole (LVPWs) was higher in AngII infusion rats, while AA treatment attenuated these alterations (Fig. 2b). Results of WGA staining showed that the cell size of cardiomyocytes was significantly increased in the myocardium of the Ang-II infusion animal model, which was significantly attenuated in AA-treated rats (Fig. 2c). To determine the effect of AA on cardiac fibrosis, heart sections were stained with Masson’s staining. In Fig. 2d, interstitial fibrosis is demonstrated by the blue areas. Quantitative data revealed increased collagen deposition in AngII-induced rats, while was significantly attenuated in AA-treated rats (Fig. 2d). Meanwhile, significant increase of ANP and β-MHC protein expression was observed in the hypertrophic rat myocardium, while their expression was inhibited in AA-treated rat (Fig. 2e). Collectively, AA treatment can inhibit AngII-induced cardiac hypertrophy and fibrosis. Reference: Nutr Metab (Lond). 2021; 18: 71. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278598/
Solvent mg/mL mM
Solubility
DMSO 59.8 122.43
DMSO:PBS (pH 7.2) (1:3) 0.3 0.51
DMF 20.0 40.92
Ethanol 10.0 20.46
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 488.70 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Yang Z, Feng L, Huang J, Zhang X, Lin W, Wang B, Cui L, Lin S, Li G. Asiatic acid protects articular cartilage through promoting chondrogenesis and inhibiting inflammation and hypertrophy in osteoarthritis. Eur J Pharmacol. 2021 Jun 24;907:174265. doi: 10.1016/j.ejphar.2021.174265. Epub ahead of print. PMID: 34174266. 2. Li J, Chen K, Huang J, Chu D, Tian M, Huang K, Ma C. Asiatic Acid Induces Endoplasmic Reticulum Stress and Activates the Grp78/IRE1α/JNK and Calpain Pathways to Inhibit Tongue Cancer Growth. Front Pharmacol. 2021 May 26;12:690612. doi: 10.3389/fphar.2021.690612. PMID: 34122116; PMCID: PMC8187906. 3. Li H, Tian X, Ruan Y, Xing J, Meng Z. Asiatic acid alleviates Ang-II induced cardiac hypertrophy and fibrosis via miR-126/PIK3R2 signaling. Nutr Metab (Lond). 2021 Jul 13;18(1):71. doi: 10.1186/s12986-021-00596-7. PMID: 34256802; PMCID: PMC8278598. 4. Fang M, Wan W, Li Q, Wan W, Long Y, Liu H, Yang X. Asiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-κB p65 mediated modulation of microglia polarization. Life Sci. 2021 Jul 15;277:119567. doi: 10.1016/j.lfs.2021.119567. Epub 2021 May 6. PMID: 33965378.
In vitro protocol:
1. Yang Z, Feng L, Huang J, Zhang X, Lin W, Wang B, Cui L, Lin S, Li G. Asiatic acid protects articular cartilage through promoting chondrogenesis and inhibiting inflammation and hypertrophy in osteoarthritis. Eur J Pharmacol. 2021 Jun 24;907:174265. doi: 10.1016/j.ejphar.2021.174265. Epub ahead of print. PMID: 34174266. 2. Li J, Chen K, Huang J, Chu D, Tian M, Huang K, Ma C. Asiatic Acid Induces Endoplasmic Reticulum Stress and Activates the Grp78/IRE1α/JNK and Calpain Pathways to Inhibit Tongue Cancer Growth. Front Pharmacol. 2021 May 26;12:690612. doi: 10.3389/fphar.2021.690612. PMID: 34122116; PMCID: PMC8187906.
In vivo protocol:
1. Li H, Tian X, Ruan Y, Xing J, Meng Z. Asiatic acid alleviates Ang-II induced cardiac hypertrophy and fibrosis via miR-126/PIK3R2 signaling. Nutr Metab (Lond). 2021 Jul 13;18(1):71. doi: 10.1186/s12986-021-00596-7. PMID: 34256802; PMCID: PMC8278598. 2. Fang M, Wan W, Li Q, Wan W, Long Y, Liu H, Yang X. Asiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-κB p65 mediated modulation of microglia polarization. Life Sci. 2021 Jul 15;277:119567. doi: 10.1016/j.lfs.2021.119567. Epub 2021 May 6. PMID: 33965378.
1: Chen R, Zhang W, Zhang M, Liu W, Feng W, Zhang Y. Asiatic acid in anticancer effects: emerging roles and mechanisms. Front Pharmacol. 2025 Feb 24;16:1545654. doi: 10.3389/fphar.2025.1545654. PMID: 40066340; PMCID: PMC11891365. 2: Liang C, Ma Y, Ding M, Gao F, Yu K, Wang S, Qu Y, Hua H, Li D. Asiatic acid and its derivatives: Pharmacological insights and applications. Eur J Med Chem. 2025 Feb 23;289:117429. doi: 10.1016/j.ejmech.2025.117429. Epub ahead of print. PMID: 40015163. 3: Varada S, Chamberlin SR, Bui L, Brandes MS, Gladen-Kolarsky N, Harris CJ, Hack W, Neff CJ, Brumbach BH, Soumyanath A, Quinn JF, Gray NE. Oral Asiatic Acid Improves Cognitive Function and Modulates Antioxidant and Mitochondrial Pathways in Female 5xFAD Mice. Nutrients. 2025 Feb 19;17(4):729. doi: 10.3390/nu17040729. PMID: 40005058; PMCID: PMC11858387. 4: Zeng W, Li H, Liu S, Luo Z, Chen J, Zhou J. Biosynthesis and bioactivities of triterpenoids from Centella asiatica: Challenges and opportunities. Biotechnol Adv. 2025 May-Jun;80:108541. doi: 10.1016/j.biotechadv.2025.108541. Epub 2025 Feb 18. PMID: 39978422. 5: Liu H, Cheng J, Ye F, Dong X, Ge W, Wang X, Zhao Y, Dan G, Chen M, Sai Y. Asiatic acid improves the damage of HaCaT cells induced by nitrogen mustard through inhibiting endoplasmic reticulum stress. Toxicol Res (Camb). 2025 Feb 17;14(1):tfaf019. doi: 10.1093/toxres/tfaf019. PMID: 39968516; PMCID: PMC11831031. 6: Zhang Q, Feng T, Chang Q, Yang D, Li Y, Shang Y, Gao W, Zhao J, Li X, Ma L, Liang Z. Exploring the potential active components and mechanisms of Tetrastigma hemsleyanum against ulcerative colitis based on network pharmacology in LPS- induced RAW264.7 cells. J Ethnopharmacol. 2025 Feb 27;342:119384. doi: 10.1016/j.jep.2025.119384. Epub 2025 Jan 23. PMID: 39863095. 7: Lee YS, Kwon RJ, Lee HS, Chung JH, Kim YS, Jeong HS, Park SJ, Lee SY, Kim T, Yoon SH. The Role of Pentacyclic Triterpenoids in Non-Small Cell Lung Cancer: The Mechanisms of Action and Therapeutic Potential. Pharmaceutics. 2024 Dec 26;17(1):22. doi: 10.3390/pharmaceutics17010022. PMID: 39861671; PMCID: PMC11768946. 8: Mahadev M, Ballal S, Shetty A, Dubey A, Shetty SS, Hebbar S, El-Zahaby SA. Development and evaluation of chitosan-coated virgin coconut oil-asiatic acid- loaded nanoemulgel for enhanced wound management. Int J Biol Macromol. 2025 Apr;299:140097. doi: 10.1016/j.ijbiomac.2025.140097. Epub 2025 Jan 21. PMID: 39848368. 9: Liu Q, Liu J, Zheng Y, Lei J, Huang J, Liu S, Liu F, Peng Q, Zhang Y, Wang J, Li Y. Quercetin mediates the therapeutic effect of Centella asiatica on psoriasis by regulating STAT3 phosphorylation to inhibit the IL-23/IL-17A axis. Nan Fang Yi Ke Da Xue Xue Bao. 2025 Jan 20;45(1):90-99. English, Chinese. doi: 10.12122/j.issn.1673-4254.2025.01.12. PMID: 39819717; PMCID: PMC11744291. 10: Sumrejkanchanakij P, Fitri AR, Pavasant P, Chareonvit S, Lin ACK, Chamni S. Asiatic acid methyl ester, a new asiaticoside derivative, induces osteogenic differentiation of hPDLCs. Arch Oral Biol. 2025 Apr;172:106175. doi: 10.1016/j.archoralbio.2025.106175. Epub 2025 Jan 11. PMID: 39813772. 11: Zhang T, Liu YJ, Zhang L, Zhou XY, Jia XH. [Reversal Roles and Its Mechanism of Asiatic Acid on Multidrug Resistance in K562/ADR Cells Through the Wnt/β-catenin Pathway]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2024 Dec;32(6):1696-1703. Chinese. doi: 10.19746/j.cnki.issn.1009-2137.2024.06.010. PMID: 39743253. 12: Chen D, Chen X, Zheng X, Zhu J, Xue T. Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus. BMC Genomics. 2024 Dec 18;25(1):1197. doi: 10.1186/s12864-024-11125-0. PMID: 39695362; PMCID: PMC11654178. 13: Qiu W, Jiang W, Su Y, Huang H, Ye J, Wang R, Tang Z, Su R. Asiatic Acid Alleviates LPS-Induced Pyroptosis and Endoplasmic Reticulum Stress-Mediated Apoptosis via Inhibiting the HMGB1/TLR4/NF-κB Pathway in Broiler Hepatocytes. J Anim Physiol Anim Nutr (Berl). 2025 Mar;109(2):508-520. doi: 10.1111/jpn.14070. Epub 2024 Nov 14. PMID: 39543935. 14: Kandaswamy K, Panda SP, Shaik MR, Hussain SA, Deepak P, Thiyagarajulu N, Jain D, Antonyraj APM, Subramanian R, Guru A, Arockiaraj J. Formulation of Asiatic acid-loaded polymeric chitosan-based hydrogel for effective MRSA infection control and enhanced wound healing in zebrafish models. Int J Biol Macromol. 2025 Mar;293:137425. doi: 10.1016/j.ijbiomac.2024.137425. Epub 2024 Nov 13. PMID: 39542332. 15: Karwowska K, Gniadek M, Urbaniak W, Petelska AD. Physicochemical and electrical properties of DPPC bilayer membranes in the presence of oleanolic or asiatic acid. Sci Rep. 2024 Nov 8;14(1):27282. doi: 10.1038/s41598-024-79234-0. PMID: 39516535; PMCID: PMC11549409. 16: Luo K, Geng Y, Oosterhuis D, de Meijer VE, Olinga P. Evaluating the antifibrotic potential of naringenin, asiatic acid, and icariin using murine and human precision-cut liver slices. Physiol Rep. 2024 Nov;12(21):e16136. doi: 10.14814/phy2.16136. PMID: 39501714; PMCID: PMC11538472. 17: Hu Y, Gu J, Jin X, Wu X, Li H, Bai L, Wu J, Li X Sr. Asiatic acid alleviates subarachnoid hemorrhage-induced brain injury in rats by inhibiting ferroptosis of neurons via targeting acyl-coenzyme a oxidase 1. Neuropharmacology. 2025 Jan 1;262:110208. doi: 10.1016/j.neuropharm.2024.110208. Epub 2024 Nov 3. PMID: 39500389. 18: Yang H, Yang B, Teng Y, Ge J, Feng X, Tian Y. Identification of α-tubulin alpha-1B chain as a target of asiatic acid using chemical proteomics in HepG2 hepatoma cells. Org Biomol Chem. 2024 Dec 11;22(48):9371-9378. doi: 10.1039/d4ob01298d. PMID: 39479883. 19: Li R, Wang C, Xu K, Zhan Z, He S, Ren J, Li F, Tao N, Li Z, Yang Z, Yu H. Asiatic acid inhibits HBV cccDNA transcription by promoting HBx degradation. Virol J. 2024 Oct 28;21(1):268. doi: 10.1186/s12985-024-02535-3. PMID: 39468627; PMCID: PMC11520515. 20: Witkowska K, Paczkowska-Walendowska M, Garbiec E, Cielecka-Piontek J. Topical Application of Centella asiatica in Wound Healing: Recent Insights into Mechanisms and Clinical Efficacy. Pharmaceutics. 2024 Sep 26;16(10):1252. doi: 10.3390/pharmaceutics16101252. PMID: 39458583; PMCID: PMC11510310.