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MedKoo Cat#: 300144 | Name: Methylprednisolone Acetate
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Methylprednisolone acetate, also known as DEPO-MEDROL, is an anti-inflammatory glucocorticoid for intramuscular, intra-articular, soft tissue or intralesional injection. It is available in three strengths: 20 mg/mL; 40 mg/mL; 80 mg/mL.

Chemical Structure

Methylprednisolone Acetate
Methylprednisolone Acetate
CAS#53-36-1

Theoretical Analysis

MedKoo Cat#: 300144

Name: Methylprednisolone Acetate

CAS#: 53-36-1

Chemical Formula: C24H32O6

Exact Mass: 416.2199

Molecular Weight: 416.51

Elemental Analysis: C, 69.21; H, 7.74; O, 23.05

Price and Availability

Size Price Availability Quantity
250mg USD 350.00 2 weeks
1g USD 750.00 2 weeks
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Related CAS #
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Synonym
Depo-Medrate; Depo-Medrin; Depo-medrol; Depomedrone; Depometicort; Lemod; Depo M-Predrol; Medrol; Medrol acetate; Mepred; Methyl prednisolone acetate; Methylprednisolone 21-acetate; Methylprednisolone acetate; NSC 48985;
IUPAC/Chemical Name
2-((6S,8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-6,10,13-trimethyl-3-oxo-6,7,8,9,10,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a]phenanthren-17-yl)-2-oxoethyl acetate
InChi Key
PLBHSZGDDKCEHR-LFYFAGGJSA-N
InChi Code
InChI=1S/C24H32O6/c1-13-9-16-17-6-8-24(29,20(28)12-30-14(2)25)23(17,4)11-19(27)21(16)22(3)7-5-15(26)10-18(13)22/h5,7,10,13,16-17,19,21,27,29H,6,8-9,11-12H2,1-4H3/t13-,16-,17-,19-,21+,22-,23-,24-/m0/s1
SMILES Code
CC(OCC([C@@]1(O)CC[C@@]2([H])[C@]3([H])C[C@H](C)C4=CC(C=C[C@]4(C)[C@@]3([H])[C@@H](O)C[C@]12C)=O)=O)=O
Appearance
White to off-white crystalline powder.
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO.
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Methylprednisolone acetate, a prednisolone derivative, is a corticosteroid hormone.
In vitro activity:
In addition to its potent anti-inflammatory and antioxidant properties, this study recently discovered a selective antiapoptotic effect of MP (methylprednisone) on oligodendrocytes via the activation of the glucocorticoid receptor (GR) and the upregulation of bcl-X(L), a splicing isoform of the bcl-x gene. The GR and STAT5 complex is present on the STAT5-binding site of the bcl-x promoter region in oligodendrocytes; the overexpression of an activated form of STAT5 prevents alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-induced oligodendrocyte cell death; and this prevention is lost when the STAT5 gene is knocked down. Reference: J Neurosci. 2009 Feb 18;29(7):2022-6. https://pubmed.ncbi.nlm.nih.gov/19228956/
In vivo activity:
This study aimed at evaluating the effect of methylprednisolone (MPA) on messenger ribonucleic acid (mRNA) expression levels in immature ovine knee joint tissue explants following interleukin (IL)1β induction and to assess responsiveness of the explants. Methylprednisolone acetate treatment significantly suppressed MMPs consistently across the cartilage (MMP1, MMP3, and MMP13), synovium (MMP1 and MMP3), and IPFP (MMP13) (all p < 0.05). Reference: Inflamm Res. 2021 Jan;70(1):99-107. https://pubmed.ncbi.nlm.nih.gov/33226449/
Solvent mg/mL mM comments
Solubility
DMF 5.0 12.00
DMSO 63.7 152.86
Ethanol 2.0 4.80
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 416.51 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Xu J, Chen S, Chen H, Xiao Q, Hsu CY, Michael D, Bao J. STAT5 mediates antiapoptotic effects of methylprednisolone on oligodendrocytes. J Neurosci. 2009 Feb 18;29(7):2022-6. doi: 10.1523/JNEUROSCI.2621-08.2009. PMID: 19228956; PMCID: PMC2662324. 2. Diem R, Hobom M, Maier K, Weissert R, Storch MK, Meyer R, Bähr M. Methylprednisolone increases neuronal apoptosis during autoimmune CNS inflammation by inhibition of an endogenous neuroprotective pathway. J Neurosci. 2003 Aug 6;23(18):6993-7000. doi: 10.1523/JNEUROSCI.23-18-06993.2003. PMID: 12904460; PMCID: PMC6740669. 3. Barton KI, Chung M, Frank CB, Shrive NG, Hart DA. Methylprednisolone acetate mitigates IL1β induced changes in matrix metalloproteinase gene expression in skeletally immature ovine explant knee tissues. Inflamm Res. 2021 Jan;70(1):99-107. doi: 10.1007/s00011-020-01421-2. Epub 2020 Nov 23. PMID: 33226449. 4. Patton JB, Bonne-Année S, Deckman J, Hess JA, Torigian A, Nolan TJ, Wang Z, Kliewer SA, Durham AC, Lee JJ, Eberhard ML, Mangelsdorf DJ, Lok JB, Abraham D. Methylprednisolone acetate induces, and Δ7-dafachronic acid suppresses, Strongyloides stercoralis hyperinfection in NSG mice. Proc Natl Acad Sci U S A. 2018 Jan 2;115(1):204-209. doi: 10.1073/pnas.1712235114. Epub 2017 Dec 4. PMID: 29203662; PMCID: PMC5776800.
In vitro protocol:
1. Xu J, Chen S, Chen H, Xiao Q, Hsu CY, Michael D, Bao J. STAT5 mediates antiapoptotic effects of methylprednisolone on oligodendrocytes. J Neurosci. 2009 Feb 18;29(7):2022-6. doi: 10.1523/JNEUROSCI.2621-08.2009. PMID: 19228956; PMCID: PMC2662324. 2. Diem R, Hobom M, Maier K, Weissert R, Storch MK, Meyer R, Bähr M. Methylprednisolone increases neuronal apoptosis during autoimmune CNS inflammation by inhibition of an endogenous neuroprotective pathway. J Neurosci. 2003 Aug 6;23(18):6993-7000. doi: 10.1523/JNEUROSCI.23-18-06993.2003. PMID: 12904460; PMCID: PMC6740669.
In vivo protocol:
1. Barton KI, Chung M, Frank CB, Shrive NG, Hart DA. Methylprednisolone acetate mitigates IL1β induced changes in matrix metalloproteinase gene expression in skeletally immature ovine explant knee tissues. Inflamm Res. 2021 Jan;70(1):99-107. doi: 10.1007/s00011-020-01421-2. Epub 2020 Nov 23. PMID: 33226449. 2. Patton JB, Bonne-Année S, Deckman J, Hess JA, Torigian A, Nolan TJ, Wang Z, Kliewer SA, Durham AC, Lee JJ, Eberhard ML, Mangelsdorf DJ, Lok JB, Abraham D. Methylprednisolone acetate induces, and Δ7-dafachronic acid suppresses, Strongyloides stercoralis hyperinfection in NSG mice. Proc Natl Acad Sci U S A. 2018 Jan 2;115(1):204-209. doi: 10.1073/pnas.1712235114. Epub 2017 Dec 4. PMID: 29203662; PMCID: PMC5776800.
1: Maman E, Yehuda C, Pritsch T, Morag G, Brosh T, Sharfman Z, Dolkart O. Detrimental Effect of Repeated and Single Subacromial Corticosteroid Injections on the Intact and Injured Rotator Cuff: A Biomechanical and Imaging Study in Rats. Am J Sports Med. 2015 Jul 27. pii: 0363546515591266. [Epub ahead of print] PubMed PMID: 26216105. 2: Rice DA, McNair PJ, Lewis GN, Dalbeth N. The effects of joint aspiration and intra-articular corticosteroid injection on flexion reflex excitability, quadriceps strength and pain in individuals with knee synovitis: a prospective observational study. Arthritis Res Ther. 2015 Jul 28;17:191. doi: 10.1186/s13075-015-0711-5. PubMed PMID: 26215105; PubMed Central PMCID: PMC4517546.