Mahanine, a carbazole alkaloid (occurs in the edible part of Micromelum minutum, Murraya koenigii and related species), is a DNA minor groove binding agent with potent anticancer activity. Mahanine down regulates cell survival factors by activation of caspase-3 through mitochondrial dependent pathway. Mahanine was found to inhibit growth of PC3 and LNCaP prostate cancer cells in a dose and time-dependent manner. Mechanistically, mahanine inhibited cell-survival pathway by dephosphorylation of PIP3 dependent kinase 1 (PDK1) thereby deactivation of Akt and downregulation of Bcl-xL. In addition, mahanine activated caspase pathway (caspases 9 and 3) and eventually cleavage of DNA repair enzyme, PARP resulting DNA fragmentation and apoptosis.
MedKoo Cat#: 406297
Name: Mahanine
CAS#: 28360-49-8
Chemical Formula: C23H25NO2
Exact Mass: 347.1885
Molecular Weight: 347.45
Elemental Analysis: C, 79.51; H, 7.25; N, 4.03; O, 9.21
The following data is based on the product molecular weight 347.45 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
