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MedKoo Cat#: 406136 | Name: Go6983
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

GO6983 is a potent protein kinase C (PKC) inhibitor. GO6893 displays cardioprotective properties; reduces polymorphonuclear leukocyte adherence and infiltration following myocardial ischemia/reperfusion injury.

Chemical Structure

Go6983
Go6983
CAS#133053-19-7

Theoretical Analysis

MedKoo Cat#: 406136

Name: Go6983

CAS#: 133053-19-7

Chemical Formula: C26H26N4O3

Exact Mass: 442.2005

Molecular Weight: 442.52

Elemental Analysis: C, 70.57; H, 5.92; N, 12.66; O, 10.85

Price and Availability

Size Price Availability Quantity
25mg USD 250.00 2 Weeks
50mg USD 450.00 2 Weeks
100mg USD 750.00 2 Weeks
200mg USD 1,250.00 2 Weeks
500mg USD 2,650.00 2 Weeks
1g USD 3,950.00 2 Weeks
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Synonym
Go6983 Go-6983; Go 6983; Goe6983 Goe-6983; Goe 6983;
IUPAC/Chemical Name
3-(1-(3-(dimethylamino)propyl)-5-methoxy-1H-indol-3-yl)-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione
InChi Key
LLJJDLHGZUOMQP-UHFFFAOYSA-N
InChi Code
InChI=1S/C26H26N4O3/c1-29(2)11-6-12-30-15-20(18-13-16(33-3)9-10-22(18)30)24-23(25(31)28-26(24)32)19-14-27-21-8-5-4-7-17(19)21/h4-5,7-10,13-15,27H,6,11-12H2,1-3H3,(H,28,31,32)
SMILES Code
O=C(C(C1=CN(CCCN(C)C)C2=C1C=C(OC)C=C2)=C3C4=CNC5=C4C=CC=C5)NC3=O
Appearance
Solid powder
Purity
>98%
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>5 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
        
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Go 6983 is a pan-PKC inhibitor against for PKCα, PKCβ, PKCγ, PKCδ and PKCζ with IC50 of 7 nM, 7 nM, 6 nM, 10 nM and 60 nM, respectively.
In vitro activity:
S892 phosphorylation of endogenous EphA2 is inhibited by the broad spectrum PKC inhibitor Go 6983 in most cancer cell lines (Fig. 5A) and some non-transformed cell lines (Fig. 5B). This implies that one or more of the kinases targeted by Go 6983 (including conventional PKCs, PKCδ and PKCζ; Table S1) are responsible for physiological and pathological EphA2 phosphorylation on S892. Reference: Cell Signal. 2020 Sep;73:109668. https://pubmed.ncbi.nlm.nih.gov/32413552/
In vivo activity:
In another series of experiments, after EADs were induced by H2O2 perfusion, rabbit myocytes were perfused with bath solution containing both Gö 6983 and H2O2. Gö 6983 effectively suppressed H2O2-induced EADs, DADs and TAs in five out of five myocytes. Five consecutive APs under control conditions, in the presence of H2O2 and after the addition of Gö 6983, are shown in Figure 3A. Reference: Int J Mol Sci. 2017 Mar 30;18(4):688. https://pubmed.ncbi.nlm.nih.gov/28358314/
Solvent mg/mL mM
Solubility
DMF 10.0 22.60
DMF:PBS (pH 7) (1:10) 0.1 0.23
DMSO 38.5 87.07
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 442.52 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Gehring MP, Pasquale EB. Protein kinase C phosphorylates the EphA2 receptor on serine 892 in the regulatory linker connecting the kinase and SAM domains. Cell Signal. 2020 Sep;73:109668. doi: 10.1016/j.cellsig.2020.109668. Epub 2020 May 13. PMID: 32413552; PMCID: PMC7668293. 2. Chen X, Lv Q, Ma J, Liu Y. PLCγ2 promotes apoptosis while inhibits proliferation in rat hepatocytes through PKCD/JNK MAPK and PKCD/p38 MAPK signalling. Cell Prolif. 2018 Jun;51(3):e12437. doi: 10.1111/cpr.12437. Epub 2018 Feb 11. PMID: 29430764; PMCID: PMC6528867. 3. Fei YD, Li W, Hou JW, Guo K, Chen XM, Chen YH, Wang Q, Xu XL, Wang YP, Li YG. Oxidative Stress-Induced Afterdepolarizations and Protein Kinase C Signaling. Int J Mol Sci. 2017 Mar 30;18(4):688. doi: 10.3390/ijms18040688. PMID: 28358314; PMCID: PMC5412274. 4. Zhang J, Wang M, Li Z, Bi X, Song J, Weng S, Fu G. NADPH oxidase activation played a critical role in the oxidative stress process in stable coronary artery disease. Am J Transl Res. 2016 Dec 15;8(12):5199-5210. PMID: 28077995; PMCID: PMC5209475.
In vitro protocol:
1. Gehring MP, Pasquale EB. Protein kinase C phosphorylates the EphA2 receptor on serine 892 in the regulatory linker connecting the kinase and SAM domains. Cell Signal. 2020 Sep;73:109668. doi: 10.1016/j.cellsig.2020.109668. Epub 2020 May 13. PMID: 32413552; PMCID: PMC7668293. 2. Chen X, Lv Q, Ma J, Liu Y. PLCγ2 promotes apoptosis while inhibits proliferation in rat hepatocytes through PKCD/JNK MAPK and PKCD/p38 MAPK signalling. Cell Prolif. 2018 Jun;51(3):e12437. doi: 10.1111/cpr.12437. Epub 2018 Feb 11. PMID: 29430764; PMCID: PMC6528867.
In vivo protocol:
1. Fei YD, Li W, Hou JW, Guo K, Chen XM, Chen YH, Wang Q, Xu XL, Wang YP, Li YG. Oxidative Stress-Induced Afterdepolarizations and Protein Kinase C Signaling. Int J Mol Sci. 2017 Mar 30;18(4):688. doi: 10.3390/ijms18040688. PMID: 28358314; PMCID: PMC5412274. 2. Zhang J, Wang M, Li Z, Bi X, Song J, Weng S, Fu G. NADPH oxidase activation played a critical role in the oxidative stress process in stable coronary artery disease. Am J Transl Res. 2016 Dec 15;8(12):5199-5210. PMID: 28077995; PMCID: PMC5209475.
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