MC3343 is a potent, non-nucleoside DNA methyltransferase (DNMT) inhibitor, exhibiting IC₅₀ values of 5.7 μM for DNMT1 and 1.7 μM for DNMT3a in cell-free assays. Compared to earlier inhibitors like RG-108 and SGI-1027, MC3343 demonstrates enhanced potency against DNMT1. In osteosarcoma (OS) models, particularly in Saos-2 cells, it effectively suppresses proliferation and promotes osteoblastic differentiation. Treatment with MC3343 leads to dose- and time-dependent reductions in DNMT1, DNMT3a, and DNMT3b protein levels. In vivo studies reveal that MC3343 synergizes with chemotherapeutic agents such as doxorubicin and cisplatin, resulting in diminished growth of patient-derived xenograft OS tumors and induction of differentiation
MedKoo Cat#: 128433
Name: MC3343
CAS#: 1535187-91-7
Chemical Formula: C27H23N7O
Exact Mass: 461.1964
Molecular Weight: 461.53
Elemental Analysis: C, 70.27; H, 5.02; N, 21.24; O, 3.47
The following data is based on the product molecular weight 461.53 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
