Cytarabine triphosphate (ara-CTP), the active metabolite of the prodrug cytarabine, exerts its bioactivity primarily through inhibition of DNA polymerase, especially DNA polymerase α, thereby blocking DNA synthesis in rapidly dividing cells. Intracellular accumulation of ara-CTP correlates with cytotoxic efficacy, and its incorporation into DNA leads to premature chain termination. In leukemic cells, intracellular concentrations of ara-CTP typically reach 0.1–1 mM following standard cytarabine dosing, which is sufficient to inhibit DNA synthesis by over 90%. Its cytotoxic potency has been quantified in vitro with IC₅₀ values in the low micromolar to nanomolar range (e.g., 0.01–10 µM) depending on the cell type
MedKoo Cat#: 125447
Name: Cytarabine triphosphate
CAS#: 13191-15-6
Chemical Formula: C9H16N3O14P3
Exact Mass: 482.9800
Molecular Weight: 483.16
Elemental Analysis: C, 22.37; H, 3.34; N, 8.70; O, 46.36; P, 19.23
The following data is based on the product molecular weight 483.16 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
