J 3-54 | CAS#4734-59-2 | LSD1 inhibitor

MedKoo Cat#: 556219 | Name: J 3-54

Description:

WARNING: This product is for research use only, not for human or veterinary use.

J 3-54, also known as LSD1-IN-24, is a selective LSD1 inhibitor with IC50 = 0.247 μM. J-3-54 can mediate the expression of programmed cell death-ligand 1 (PD-L1), enhance T cell killing response, and can be used in cancer research.

Chemical Structure

J 3-54

CAS#4734-59-2

Theoretical Analysis

MedKoo Cat#: 556219

Name: J 3-54

CAS#: 4734-59-2

Chemical Formula: C18H20N2OS

Exact Mass: 312.1296

Molecular Weight: 312.43

Elemental Analysis: C, 69.20; H, 6.45; N, 8.97; O, 5.12; S, 10.26

Price and Availability

Size	Price	Availability
10mg	USD 140.00	Ready to ship
25mg	USD 210.00	Ready to ship
50mg	USD 350.00	Ready to ship
100mg	USD 550.00	Ready to ship
200mg	USD 950.00	Ready to ship
500mg	USD 1,650.00	Ready to ship
1g	USD 2,950.00	Ready to ship
2g	USD 5,250.00	Ready to ship

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Related CAS #

No Data

Synonym

LSD1-IN-24; J 3-54; J-3-54; J3-54; J 354; J-354; J354; J 3-54;

IUPAC/Chemical Name

4-(2-(10H-phenothiazin-10-yl)ethyl)morpholine

InChi Key

VJQXIWLVJMDRGV-UHFFFAOYSA-N

InChi Code

InChI=1S/C18H20N2OS/c1-3-7-17-15(5-1)20(10-9-19-11-13-21-14-12-19)16-6-2-4-8-18(16)22-17/h1-8H,9-14H2

SMILES Code

N1(CCN2C3=C(SC4=C2C=CC=C4)C=CC=C3)CCOCC1

Appearance

To be determined

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot#A23T12K18

QC Data

View QC data: current batch, Lot#A23T12K18

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

J-3-54 is a selective LSD1 inhibitor with IC50 = 0.247 μM.

In vitro activity:

This study characterized chlorpromazine as an LSD1 inhibitor (IC50 = 5.135 μM) and synthesized a series of chlorpromazine derivatives. Of them, J-3-54 (IC50 = 0.247 μM) was the most potent. J-3-54 inhibited LSD1 in the cellular level and downregulated the expression of PD-L1 in BGC-823 and MFC cells to enhance T-cell killing response. Reference: J Med Chem. 2023 Mar 23;66(6):3896-3916. https://pubmed.ncbi.nlm.nih.gov/36856685/

In vivo activity:

J-3-54 may serve as a lead compound for further development to activate T-cell immunity in gastric cancer. In immunocompetent mice, J-3-54 inhibited MFC cell proliferation without significant toxicity Reference: J Med Chem. 2023 Mar 23;66(6):3896-3916. https://pubmed.ncbi.nlm.nih.gov/36856685/

	Solvent	mg/mL	mM
Solubility
	DMSO	100.0	320.07

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 312.43 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Dai XJ, Zhao LJ, Yang LH, Guo T, Xue LP, Ren HM, Yin ZL, Xiong XP, Zhou Y, Ji SK, Liu HM, Liu HM, Liu Y, Zheng YC. Phenothiazine-Based LSD1 Inhibitor Promotes T-Cell Killing Response of Gastric Cancer Cells. J Med Chem. 2023 Mar 23;66(6):3896-3916. doi: 10.1021/acs.jmedchem.2c01593. Epub 2023 Mar 1. PMID: 36856685.

In vitro protocol:

In vivo protocol:

Dai XJ, Zhao LJ, Yang LH, Guo T, Xue LP, Ren HM, Yin ZL, Xiong XP, Zhou Y, Ji SK, Liu HM, Liu HM, Liu Y, Zheng YC. Phenothiazine-Based LSD1 Inhibitor Promotes T-Cell Killing Response of Gastric Cancer Cells. J Med Chem. 2023 Mar 23;66(6):3896-3916. doi: 10.1021/acs.jmedchem.2c01593. Epub 2023 Mar 1. PMID: 36856685.