MedKoo Cat#: 130358 | Name: BMPH
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

BMPH is a heterobifunctional crosslinker responsible for the conjugation of glycoproteins and sulfhydryl-containing peptides or proteins. BMPH contains sulfhydryl-reactive maleimide and carbonyl-reactive hydrazide moieties, forming stable thioether bonds and hydrazone bonds respectively. BMPH has been used as a crosslinker in the biotinylation of antibodies.

Chemical Structure

BMPH
BMPH
CAS#359436-61-6

Theoretical Analysis

MedKoo Cat#: 130358

Name: BMPH

CAS#: 359436-61-6

Chemical Formula: C9H10F3N3O5

Exact Mass: 0.0000

Molecular Weight: 297.19

Elemental Analysis: C, 36.37; H, 3.39; F, 19.18; N, 14.14; O, 26.92

Price and Availability

Size Price Availability Quantity
100mg USD 350.00 2 Weeks
250mg USD 650.00 2 Weeks
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Related CAS #
No Data
Synonym
BMPH; N-(β-maleimidopropionic acid) hydrazide, trifluoroacetic acid salt; 3-Maleimidopropionic acid hydrazonium trifluoroacetate: BMPH crosslinker
IUPAC/Chemical Name
3-(2,5-dioxopyrrol-1-yl)propanehydrazide; 2,2,2-trifluoroacetic acid
InChi Key
BADCXPKRBUEEMA-UHFFFAOYSA-N
InChi Code
InChI=1S/C7H9N3O3.C2HF3O2/c8-9-5(11)3-4-10-6(12)1-2-7(10)13;3-2(4,5)1(6)7/h1-2H,3-4,8H2,(H,9,11);(H,6,7)
SMILES Code
O=C(NN)CCN1C(C=CC1=O)=O.O=C(O)C(F)(F)F
Appearance
To be determined
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 297.19 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1. Berg EA, Fishman JB. Biotinylating Antibodies Using Biotin-Long Chain (LC) Hydrazide. Cold Spring Harb Protoc. 2020 Jan 2;2020(1):099275. doi: 10.1101/pdb.prot099275. PMID: 31896609. 2. Ozvegy-Laczka C, Laczkó R, Hegedus C, Litman T, Várady G, Goda K, Hegedus T, Dokholyan NV, Sorrentino BP, Váradi A, Sarkadi B. Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter. J Biol Chem. 2008 Sep 19;283(38):26059-70. doi: 10.1074/jbc.M803230200. Epub 2008 Jul 21. PMID: 18644784; PMCID: PMC3258862. 3. Ishmael SS, Ishmael FT, Jones AD, Bond JS. Protease domain glycans affect oligomerization, disulfide bond formation, and stability of the meprin A metalloprotease homo-oligomer. J Biol Chem. 2006 Dec 8;281(49):37404-15. doi: 10.1074/jbc.M602769200. Epub 2006 Oct 13. PMID: 17040911.