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MedKoo Cat#: 122981 | Name: Hygromycin A
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Hygromycin A is an aminoglycoside antibiotic that exhibits potent antibacterial activity, particularly against Gram-positive bacteria. Its bioactivity is primarily attributed to its ability to inhibit bacterial protein synthesis by interfering with ribosomal function. Several studies have reported its minimum inhibitory concentration (MIC) values against different bacterial strains. For example, Hygromycin A has been shown to inhibit the growth of Staphylococcus aureus and Enterococcus faecalis at MIC values typically in the low micromolar range. It is also known to be effective against Mycobacterium tuberculosis and other pathogenic bacteria resistant to conventional antibiotics.

Chemical Structure

Hygromycin A
Hygromycin A
CAS#6379-56-2

Theoretical Analysis

MedKoo Cat#: 122981

Name: Hygromycin A

CAS#: 6379-56-2

Chemical Formula: C23H29NO12

Exact Mass: 511.1700

Molecular Weight: 511.48

Elemental Analysis: C, 54.01; H, 5.72; N, 2.74; O, 37.54

Price and Availability

Size Price Availability Quantity
50mg USD 150.00 Ready to ship
100mg USD 250.00 Ready to ship
200mg USD 450.00 Ready to ship
500mg USD 950.00 Ready to ship
1g USD 1,650.00 Ready to ship
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Related CAS #
No Data
Synonym
Homomycin; Hygromycin; (-)-Hygromycin A; 1703-18B; Antibiotic WS 1627B; Hygromycin A; Totomycin
IUPAC/Chemical Name
(E)-3-(4-(((2S,3S,4S,5S)-5-acetyl-3,4-dihydroxytetrahydrofuran-2-yl)oxy)-3-hydroxyphenyl)-2-methyl-N-((3aS,4R,5R,6S,7R,7aR)-4,6,7-trihydroxyhexahydrobenzo[d][1,3]dioxol-5-yl)acrylamide
InChi Key
YQYJSBFKSSDGFO-IIHALWDASA-N
InChi Code
InChI=1S/C23H29NO12/c1-8(22(32)24-13-14(27)16(29)21-20(15(13)28)33-7-34-21)5-10-3-4-12(11(26)6-10)35-23-18(31)17(30)19(36-23)9(2)25/h3-6,13-21,23,26-31H,7H2,1-2H3,(H,24,32)/b8-5+/t13-,14+,15-,16-,17+,18+,19-,20+,21-,23-/m1/s1
SMILES Code
O[C@H]1[C@]2([C@@]([H])([C@H](O)[C@@H](O)[C@H]1NC(/C(C)=C/C3=CC(O)=C(O[C@H]4[C@H]([C@@H]([C@H](O4)C(C)=O)O)O)C=C3)=O)OCO2)[H]
Appearance
To be determined
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Additionally, Hygromycin A has demonstrated selective cytotoxicity in certain mammalian cell assays, although its primary use remains in antimicrobial applications. However, its bioactivity profile is distinct from Hygromycin B, which is widely used as a selection agent in molecular biology.

Preparing Stock Solutions

The following data is based on the product molecular weight 511.48 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
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PMID: 34117118; PMCID: PMC8370747. 8: Scindiya M, Malathi P, Kaverinathan K, Sundar AR, Viswanathan R. Knock-down of glucose transporter and sucrose non-fermenting gene in the hemibiotrophic fungus Colletotrichum falcatum causing sugarcane red rot. Mol Biol Rep. 2021 Mar;48(3):2053-2061. doi: 10.1007/s11033-021-06140-3. Epub 2021 Mar 3. PMID: 33660095. 9: Zimmermannova O, Felcmanova K, Sacka L, Colinet AS, Morsomme P, Sychrova H. K+-specific importers Trk1 and Trk2 play different roles in Ca2+ homeostasis and signalling in Saccharomyces cerevisiae cells. FEMS Yeast Res. 2021 Apr 7;21(3):foab015. doi: 10.1093/femsyr/foab015. PMID: 33640956. 10: Robinson KA, Dunn M, Hussey SP, Fritz-Laylin LK. Identification of antibiotics for use in selection of the chytrid fungi Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans. PLoS One. 2020 Oct 20;15(10):e0240480. doi: 10.1371/journal.pone.0240480. PMID: 33079945; PMCID: PMC7575076. 11: Lozano GL, Guan C, Cao Y, Borlee BR, Broderick NA, Stabb EV, Handelsman J. A Chemical Counterpunch: Chromobacterium violaceum ATCC 31532 Produces Violacein in Response to Translation-Inhibiting Antibiotics. mBio. 2020 May 19;11(3):e00948-20. doi: 10.1128/mBio.00948-20. PMID: 32430474; PMCID: PMC7240160. 12: Zhang K, Su J, Xu M, Zhou Z, Zhu X, Ma X, Hou J, Tan L, Zhu Z, Cai H, Liu F, Sun H, Gu P, Li C, Liang Y, Zhao W, Sun C, Fu Y. A common wild rice-derived BOC1 allele reduces callus browning in indica rice transformation. Nat Commun. 2020 Jan 23;11(1):443. doi: 10.1038/s41467-019-14265-0. PMID: 31974373; PMCID: PMC6978460. 13: Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, Öztürk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B. Split intein-mediated selection of cells containing two plasmids using a single antibiotic. Nat Commun. 2019 Oct 31;10(1):4967. doi: 10.1038/s41467-019-12911-1. Erratum in: Nat Commun. 2020 Jan 9;11(1):276. PMID: 31672972; PMCID: PMC6823396. 14: Jillette N, Du M, Zhu JJ, Cardoz P, Cheng AW. Split selectable markers. Nat Commun. 2019 Oct 31;10(1):4968. doi: 10.1038/s41467-019-12891-2. PMID: 31672965; PMCID: PMC6823381. 15: Lu J, Shi Y, Li W, Chen S, Wang Y, He X, Yin X. RcPAL, a key gene in lignin biosynthesis in Ricinus communis L. BMC Plant Biol. 2019 May 6;19(1):181. doi: 10.1186/s12870-019-1777-z. PMID: 31060493; PMCID: PMC6501403. 16: Ortega-Escalante JA, Kwok O, Miller SM. New Selectable Markers for Volvox carteri Transformation. Protist. 2019 Feb;170(1):52-63. doi: 10.1016/j.protis.2018.11.002. Epub 2018 Nov 15. PMID: 30576875. 17: Balabanova LA, Shkryl YN, Slepchenko LV, Yugay YA, Gorpenchenko TY, Kirichuk NN, Khudyakova YV, Bakunina IY, Podvolotskaya AB, Bulgakov VP, Seitkalieva AV, Son OM, Tekutyeva LA. Development of host strains and vector system for an efficient genetic transformation of filamentous fungi. Plasmid. 2019 Jan;101:1-9. doi: 10.1016/j.plasmid.2018.11.002. Epub 2018 Nov 19. PMID: 30465791. 18: Hawer H, Ütkür K, Arend M, Mayer K, Adrian L, Brinkmann U, Schaffrath R. Importance of diphthamide modified EF2 for translational accuracy and competitive cell growth in yeast. PLoS One. 2018 Oct 18;13(10):e0205870. doi: 10.1371/journal.pone.0205870. PMID: 30335802; PMCID: PMC6193676. 19: Lv S, Chen X, Mou C, Dai S, Bian Y, Kang H. Agrobacterium-mediated transformation of the ascomycete mushroom Morchella importuna using polyubiquitin and glyceraldehyde-3-phosphate dehydrogenase promoter-based binary vectors. World J Microbiol Biotechnol. 2018 Sep 14;34(10):148. doi: 10.1007/s11274-018-2529-1. PMID: 30218324. 20: Cabral GB, Carneiro VTC, Gomes ACMM, Lacerda AL, Martinelli AP, Dusi DMA. Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics. An Acad Bras Cienc. 2018 Apr-Jun;90(2):1789-1797. doi: 10.1590/0001-3765201820170842. PMID: 29898118.