RapiFluor-MS | CAS#1429047-69-7 | labelling reagent

MedKoo Cat#: 130101 | Name: RapiFluor-MS

Description:

WARNING: This product is for research use only, not for human or veterinary use.

RapiFluor-MS is a highly sensitive glycosylamine labelling reagent for the detection of O-glycans.

Chemical Structure

RapiFluor-MS

CAS#1429047-69-7

Theoretical Analysis

MedKoo Cat#: 130101

Name: RapiFluor-MS

CAS#: 1429047-69-7

Chemical Formula: C21H25N5O5

Exact Mass: 427.1856

Molecular Weight: 427.46

Elemental Analysis: C, 59.01; H, 5.90; N, 16.38; O, 18.71

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.

Bulk Inquiry

Related CAS #

No Data

Synonym

RapiFluor-MS; RapiFluorMS; RapiFluor MS; RFMS

IUPAC/Chemical Name

2,5-Dioxopyrrolidin-1-yl (2-((2-(diethylamino)ethyl)carbamoyl)quinolin-6-yl)carbamate

InChi Key

BWBDAEIIXBEFSS-UHFFFAOYSA-N

InChi Code

InChI=1S/C21H25N5O5/c1-3-25(4-2)12-11-22-20(29)17-7-5-14-13-15(6-8-16(14)24-17)23-21(30)31-26-18(27)9-10-19(26)28/h5-8,13H,3-4,9-12H2,1-2H3,(H,22,29)(H,23,30)

SMILES Code

O=C(ON1C(CCC1=O)=O)NC2=CC=C3N=C(C(NCCN(CC)CC)=O)C=CC3=C2

Appearance

To be determined

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 427.46 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

1. Shipman J, Sommers C, Keire DA, Chen K, Zhu H. Comprehensive N-Glycan Mapping using Parallel Reaction Monitoring LC-MS/MS. Pharm Res. 2022 Dec 13. doi: 10.1007/s11095-022-03453-1. Epub ahead of print. PMID: 36513905. 2. Deriš H, Cindrić A, Lauber M, Petrović T, Bielik A, Taron CH, van Wingerden M, Lauc G, Trbojević-Akmačić I. Robustness and repeatability of GlycoWorks RapiFluor-MS IgG N-glycan profiling in a long-term high-throughput glycomic study. Glycobiology. 2021 Sep 20;31(9):1062-1067. doi: 10.1093/glycob/cwab050. PMID: 34132802. 3. Upton R, Duffy J, Clawson S, Firth D. Evaluating N-Glycosylation of a Therapeutic Monoclonal Antibody Using UHPLC-FLR-MS with RapiFluor-MS Labeling. Methods Mol Biol. 2021;2271:189-203. doi: 10.1007/978-1-0716-1241-5_14. PMID: 33908009. 4. Helali Y, Sharma S, Vandeput M, Welba D, Van Antwerpen P, Marchant A, Delporte C. Fc Glycosylation Characterization of Human Immunoglobulins G Using Immunocapture and LC-MS. Methods Mol Biol. 2021;2271:57-71. doi: 10.1007/978-1-0716-1241-5_4. PMID: 33907999. 5. Pallister EG, Choo MSF, Walsh I, Tai JN, Tay SJ, Yang YS, Ng SK, Rudd PM, Flitsch SL, Nguyen-Khuong T. Utility of Ion-Mobility Spectrometry for Deducing Branching of Multiply Charged Glycans and Glycopeptides in a High-Throughput Positive ion LC-FLR-IMS-MS Workflow. Anal Chem. 2020 Dec 1;92(23):15323-15335. doi: 10.1021/acs.analchem.0c01954. Epub 2020 Nov 9. PMID: 33166117. 6. Messina A, Palmigiano A, Esposito F, Fiumara A, Bordugo A, Barone R, Sturiale L, Jaeken J, Garozzo D. HILIC-UPLC-MS for high throughput and isomeric N-glycan separation and characterization in Congenital Disorders Glycosylation and human diseases. Glycoconj J. 2021 Apr;38(2):201-211. doi: 10.1007/s10719-020-09947-7. Epub 2020 Sep 11. PMID: 32915358. 7. Szabo Z, Khan SH, Widdowson PJ, Reusch D, Viner R, Carillo S, Huhmer A, Bones J. A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid. Anal Chim Acta. 2020 Feb 22;1099:155-164. doi: 10.1016/j.aca.2019.11.055. Epub 2019 Nov 26. PMID: 31986272. 8. Keser T, Pavić T, Lauc G, Gornik O. Comparison of 2-Aminobenzamide, Procainamide and RapiFluor-MS as Derivatizing Agents for High-Throughput HILIC-UPLC-FLR-MS N-glycan Analysis. Front Chem. 2018 Jul 26;6:324. doi: 10.3389/fchem.2018.00324. PMID: 30094234; PMCID: PMC6070730. 9. Vainauskas S, Kirk CH, Petralia L, Guthrie EP, McLeod E, Bielik A, Luebbers A, Foster JM, Hokke CH, Rudd PM, Shi X, Taron CH. A novel broad specificity fucosidase capable of core α1-6 fucose release from N-glycans labeled with urea-linked fluorescent dyes. Sci Rep. 2018 Jun 22;8(1):9504. doi: 10.1038/s41598-018-27797-0. PMID: 29934601; PMCID: PMC6015026. 10. Reed CE, Fournier J, Vamvoukas N, Koza SM. Automated Preparation of MS-Sensitive Fluorescently Labeled N-Glycans with a Commercial Pipetting Robot. SLAS Technol. 2018 Dec;23(6):550-559. doi: 10.1177/2472630318762384. Epub 2018 Apr 4. PMID: 29618278; PMCID: PMC6249649. 11. Zhou S, Veillon L, Dong X, Huang Y, Mechref Y. Direct comparison of derivatization strategies for LC-MS/MS analysis of N-glycans. Analyst. 2017 Nov 20;142(23):4446-4455. doi: 10.1039/c7an01262d. PMID: 29085933; PMCID: PMC5696090. 12. Hilliard M, Alley WR Jr, McManus CA, Yu YQ, Hallinan S, Gebler J, Rudd PM. Glycan characterization of the NIST RM monoclonal antibody using a total analytical solution: From sample preparation to data analysis. MAbs. 2017 Nov/Dec;9(8):1349-1359. doi: 10.1080/19420862.2017.1377381. Epub 2017 Sep 12. PMID: 28895795; PMCID: PMC5680791. 13. Furuki K, Toyo'oka T, Ban K. Highly sensitive glycosylamine labelling of O-glycans using non-reductive β-elimination. Anal Bioanal Chem. 2017 Mar;409(9):2269-2283. doi: 10.1007/s00216-016-0171-z. Epub 2017 Jan 14. PMID: 28091715.