5BAPTA-PEG3-Haloligand is a chemigenetic calcium ion (Ca2+) indicator used for the imaging of cells. The use of the 5BAPTA-PEG3-Haloligand has allowed for bright and sensitive ion indicators for cell imaging that is lacking with the use of fully protein-based indicators. 5BAPTA-PEG3-Haloligand is based on the genetic insertion of circularly permuted green fluorescent protein into HaloTag protein self-labeled with a ligand containing the Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid.
MedKoo Cat#: 130009
Name: 5BAPTA-PEG3-Haloligand
CAS#: N/A
Chemical Formula: C37H51ClN2O15
Exact Mass: 798.2978
Molecular Weight: 799.26
Elemental Analysis: C, 55.60; H, 6.43; Cl, 4.44; N, 3.50; O, 30.03
The following data is based on the product molecular weight 799.26 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
