MedKoo Cat#: 556170 | Name: DFHO
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

DFHO is a RFP fluorophore mimic for imaging RNA in living cells.

Chemical Structure

DFHO
DFHO
CAS#1420815-34-4

Theoretical Analysis

MedKoo Cat#: 556170

Name: DFHO

CAS#: 1420815-34-4

Chemical Formula: C12H9F2N3O3

Exact Mass: 281.0612

Molecular Weight: 281.22

Elemental Analysis: C, 51.25; H, 3.23; F, 13.51; N, 14.94; O, 17.07

Price and Availability

Size Price Availability Quantity
10mg USD 650.00 2 Weeks
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Related CAS #
No Data
Synonym
DFHO; DA-62813; DA62813; DA 62813;
IUPAC/Chemical Name
4-(3,5-Difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime
InChi Key
MZFHSQVSFUWKDU-JDCBMBFUSA-N
InChi Code
InChI=1S/C12H9F2N3O3/c1-17-10(5-15-20)16-9(12(17)19)4-6-2-7(13)11(18)8(14)3-6/h2-5,18,20H,1H3/b9-4+,15-5+
SMILES Code
O=C1/C(N=C(/C=N/O)N1C)=C\C2=CC(F)=C(O)C(F)=C2
Appearance
To be determined
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 281.22 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Gu Y, Huang LJ, Zhao W, Zhang TT, Cui MR, Yang XJ, Zhao XL, Chen HY, Xu JJ. Living-Cell MicroRNA Imaging with Self-Assembling Fragments of Fluorescent Protein-Mimic RNA Aptamer. ACS Sens. 2021 Jun 25;6(6):2339-2347. doi: 10.1021/acssensors.1c00453. Epub 2021 May 24. PMID: 34028262. 2: Kim H, Jaffrey SR. A Fluorogenic RNA-Based Sensor Activated by Metabolite- Induced RNA Dimerization. Cell Chem Biol. 2019 Dec 19;26(12):1725-1731.e6. doi: 10.1016/j.chembiol.2019.09.013. Epub 2019 Oct 17. PMID: 31631009; PMCID: PMC6939632. 3: Filonov GS, Song W, Jaffrey SR. Spectral Tuning by a Single Nucleotide Controls the Fluorescence Properties of a Fluorogenic Aptamer. Biochemistry. 2019 Mar 26;58(12):1560-1564. doi: 10.1021/acs.biochem.9b00048. Epub 2019 Mar 11. PMID: 30838859. 4: Warner KD, Sjekloća L, Song W, Filonov GS, Jaffrey SR, Ferré-D'Amaré AR. A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat Chem Biol. 2017 Nov;13(11):1195-1201. doi: 10.1038/nchembio.2475. Epub 2017 Sep 25. PMID: 28945234; PMCID: PMC5663454.