N4-Methylcytidine | Nucleoside | CAS#10578-79-7

MedKoo Cat#: 122472 | Name: N4-Methylcytidine

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

N4-Methylcytidine is a nucleoside analogue that inhibits synthesis of human mitochondrial DNA by inhibiting bacterial enzyme uridine-cytidine kinase.

Chemical Structure

N4-Methylcytidine

CAS#10578-79-7

Theoretical Analysis

MedKoo Cat#: 122472

Name: N4-Methylcytidine

CAS#: 10578-79-7

Chemical Formula: C10H15N3O5

Exact Mass: 257.1012

Molecular Weight: 257.25

Elemental Analysis: C, 46.69; H, 5.88; N, 16.33; O, 31.10

Price and Availability

Size	Price	Availability	Quantity
100mg	USD 450.00	2 Weeks
250mg	USD 750.00	2 Weeks

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Related CAS #

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Synonym

N4-Methylcytidine; Cytidine, N-methyl-; N(4)-Methylcytidine

IUPAC/Chemical Name

1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-4-(methylamino)pyrimidin-2(1H)-one

InChi Key

LZCNWAXLJWBRJE-ZOQUXTDFSA-N

InChi Code

InChI=1S/C10H15N3O5/c1-11-6-2-3-13(10(17)12-6)9-8(16)7(15)5(4-14)18-9/h2-3,5,7-9,14-16H,4H2,1H3,(H,11,12,17)/t5-,7-,8-,9-/m1/s1

SMILES Code

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=CC(NC)=NC2=O)O1

Appearance

To be determined

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 257.25 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

1: Tieu Ngoc LN, Jung Park S, Thi Huong T, Lee KH, Kang H. N4-methylcytidine ribosomal RNA methylation in chloroplasts is crucial for chloroplast function, development, and abscisic acid response in Arabidopsis. J Integr Plant Biol. 2021 Mar;63(3):570-582. doi: 10.1111/jipb.13009. Epub 2020 Sep 30. PMID: 32876986. 2: Van Haute L, Hendrick AG, D'Souza AR, Powell CA, Rebelo-Guiomar P, Harbour ME, Ding S, Fearnley IM, Andrews B, Minczuk M. METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis. Nucleic Acids Res. 2019 Nov 4;47(19):10267-10281. doi: 10.1093/nar/gkz735. PMID: 31665743; PMCID: PMC6821322. 3: Mao S, Sekula B, Ruszkowski M, Ranganathan SV, Haruehanroengra P, Wu Y, Shen F, Sheng J. Base pairing, structural and functional insights into N4-methylcytidine (m4C) and N4,N4-dimethylcytidine (m42C) modified RNA. Nucleic Acids Res. 2020 Oct 9;48(18):10087-10100. doi: 10.1093/nar/gkaa737. PMID: 32941619; PMCID: PMC7544196. 4: Zheng YY, Mao S, Sheng J. Synthesis of N⁴ -Methylcytidine (m⁴ C) and N⁴ ,N⁴ -Dimethylcytidine (m⁴₂ C) Modified RNA. Curr Protoc. 2021 Sep;1(9):e248. doi: 10.1002/cpz1.248. PMID: 34529364. 5: Cheng MY, You XJ, Ding JH, Dai Y, Chen MY, Yuan BF, Feng YQ. Novel dual methylation of cytidines in the RNA of mammals. Chem Sci. 2021 May 7;12(23):8149-8156. doi: 10.1039/d1sc01972d. PMID: 34194705; PMCID: PMC8208307. 6: Dubin DT, Taylor RH, Davenport LW. Methylation status of 13S ribosomal RNA from hamster mitochondria: the presence of a novel riboside, N4-methylcytidine. Nucleic Acids Res. 1978 Nov;5(11):4385-97. doi: 10.1093/nar/5.11.4385. PMID: 724519; PMCID: PMC342756. 7: Suzuki T, Moriyama K, Otsuka C, Loakes D, Negishi K. Template properties of mutagenic cytosine analogues in reverse transcription. Nucleic Acids Res. 2006;34(22):6438-49. doi: 10.1093/nar/gkl761. Epub 2006 Nov 27. PMID: 17130163; PMCID: PMC1702492. 8: Biernat J, Ciesiołka J, Górnicki P, Adamiak RW, Kryzosiak WJ, Wiewiórowski M. New observations concerning the chloroacetaldehyde reaction with some tRNA constituents. Stable intermediates, kinetics and selectivity of the reaction. Nucleic Acids Res. 1978 Mar;5(3):789-804. doi: 10.1093/nar/5.3.789. PMID: 25420; PMCID: PMC342024.