PFI-5 has a unique chemotype relative to the current SMYD2 chemical probes LLY-507 and BAY-598. PFI-5 inhibits in vitro methylation of p53K370 with IC50 = 9 nM and has more than 100-fold selectivity over other histone methyltransferases and other non-epigenetic targets. PFI-5 inhibits the methylation of p53K370 in cells with IC50 = 0.9 µM.
MedKoo Cat#: 121035
Name: PFI-5
CAS#: Unknown
Chemical Formula: C28H38N8O
Exact Mass: 502.3169
Molecular Weight: 502.67
Elemental Analysis: C, 66.90; H, 7.62; N, 22.29; O, 3.18
The following data is based on the product molecular weight 502.67 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
