Synonym
OG-488 SE; OG 488, SE; OregonGreen488SuccinimidylEster; OREGON GREEN 488 SUCCINIMIDYL ESTER; Difluorocarboxyfluorescein NHS Ester, 5-isomer
IUPAC/Chemical Name
2-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoic acid
InChi Key
RPOLNGNGJOEMSR-UHFFFAOYSA-N
InChi Code
InChI=1S/C25H13F2NO9/c26-15-6-13-19(8-17(15)29)36-20-9-18(30)16(27)7-14(20)23(13)11-2-1-10(5-12(11)24(33)34)25(35)37-28-21(31)3-4-22(28)32/h1-2,5-9,29H,3-4H2,(H,33,34)
SMILES Code
O=C(C1=CC(C(O)=O)=C(C(C2=C(OC3=C4)C=C(O)C(F)=C2)=C3C=C(F)C4=O)C=C1)ON5C(CCC5=O)=O
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
509.37
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1. Guilini, C., Baehr, C., Schaeffer, E., Gizzi, P., Rufi, F., Haiech, J., ... & Galzi, J. L. (2015). New fluorescein precursors for live bacteria detection. Analytical chemistry, 87(17), 8858-8866.
2. Ji J, Rosenzweig N, Griffin C, Rosenzweig Z. Synthesis and application of submicrometer fluorescence sensing particles for lysosomal pH measurements in murine macrophages. Anal Chem. 2000 Aug 1;72(15):3497-503. doi: 10.1021/ac000080p. PMID: 10952534.
3. Orte A, Crovetto L, Talavera EM, Boens N, Alvarez-Pez JM. Absorption and emission study of 2',7'-difluorofluorescein and its excited-state buffer-mediated proton exchange reactions. J Phys Chem A. 2005 Feb 10;109(5):734-47. doi: 10.1021/jp046786v. PMID: 16838941.
