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MedKoo Cat#: 406155 | Name: IC-87114
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

IC-87114 is a potent and ATP-competitive PI3K p110δ isoform-selective inhibitor (IC50 = 60 nM). It Inhibits p110α, p110β and p110γ only at much higher concentrations. It does not inhibit other PIK-related kinases such as ATM, ATR, DNA-PK, and mTOR even at concentrations up to 100 µM.

Chemical Structure

IC-87114
IC-87114
CAS#371242-69-2

Theoretical Analysis

MedKoo Cat#: 406155

Name: IC-87114

CAS#: 371242-69-2

Chemical Formula: C22H19N7O

Exact Mass: 397.1651

Molecular Weight: 397.44

Elemental Analysis: C, 66.49; H, 4.82; N, 24.67; O, 4.03

Price and Availability

Size Price Availability Quantity
100mg USD 450.00 2 Weeks
200mg USD 750.00 2 Weeks
500mg USD 1,350.00 2 Weeks
1g USD 2,050.00 2 Weeks
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Related CAS #
No Data
Synonym
IC87114; IC-87114; IC 87114.
IUPAC/Chemical Name
2-((6-amino-9H-purin-9-yl)methyl)-5-methyl-3-o-tolylquinazolin-4(3H)-one
InChi Key
GNWHRHGTIBRNSM-UHFFFAOYSA-N
InChi Code
InChI=1S/C22H19N7O/c1-13-6-3-4-9-16(13)29-17(27-15-8-5-7-14(2)18(15)22(29)30)10-28-12-26-19-20(23)24-11-25-21(19)28/h3-9,11-12H,10H2,1-2H3,(H2,23,24,25)
SMILES Code
O=C1N(C2=CC=CC=C2C)C(CN3C=NC4=C(N)N=CN=C34)=NC5=C1C(C)=CC=C5
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
        
Product Data
Product Data
Instruction
View handling instruction
Biological target:
IC-87114 is a potent and selective PI3Kδ inhibitor with IC50 of 0.5 μM.
In vitro activity:
A specific phosphatidylinositol 3-kinases δ inhibitor (IC87114) or genetic p110δ inactivation upregulated MMP-12 expression and c-Jun phosphorylation (n=6; P<0.05 versus wild-type macrophages). IC87114 also increased activator protein-1 DNA-binding activity (n=6; P<0.001 versus control) and enhanced the effect of tumor necrosis factor-α on activator protein-1-binding activity (n=5; P<0.01 versus tumor necrosis factor-α treatment groups). Knockdown of c-Jun suppressed the effect of the IC87114 and tumor necrosis factor-α on MMP-12 mRNA expression (n=5 in each group; P<0.01 versus scrRNA treatment groups). Reference: Arterioscler Thromb Vasc Biol. 2015 Feb;35(2):368-77. https://pubmed.ncbi.nlm.nih.gov/25503990/
In vivo activity:
VV protein expression within the tumor was significantly enhanced by IC87114 at both 3 days (Figures 4C and 4E) and 7 days (Figures 4D and 4E) post-infection. Analysis of the spleen of mice treated with one dose of virus 3 h post-infection demonstrated an enhanced accumulation of VV protein in mice treated with vehicle buffer compared to IC87114 (Figures 4F–4H), consistent with the notion that IC87114 leads to a reduced early clearance of VVL15 by splenic macrophages and indicative of a dampening of the innate immune response to viral infection by inhibition of PI3Kδ. Reference: Mol Ther. 2020 May 6;28(5):1263-1275. https://pubmed.ncbi.nlm.nih.gov/32145202/
Solvent mg/mL mM
Solubility
DMF 30.0 75.48
DMSO 13.6 34.10
DMSO:PBS (pH 7.2) (1:1) 0.5 1.26
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 397.44 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Zheng L, Xing L, Zeng C, Wu T, Gui Y, Li W, Lan T, Yang Y, Gu Q, Qi C, Zhang Q, Tang F, He X, Wang L. Inactivation of PI3Kδ induces vascular injury and promotes aneurysm development by upregulating the AP-1/MMP-12 pathway in macrophages. Arterioscler Thromb Vasc Biol. 2015 Feb;35(2):368-77. doi: 10.1161/ATVBAHA.114.304365. Epub 2014 Dec 11. PMID: 25503990. 2. Sadhu C, Masinovsky B, Dick K, Sowell CG, Staunton DE. Essential role of phosphoinositide 3-kinase delta in neutrophil directional movement. J Immunol. 2003 Mar 1;170(5):2647-54. doi: 10.4049/jimmunol.170.5.2647. PMID: 12594293. 3. Ferguson MS, Chard Dunmall LS, Gangeswaran R, Marelli G, Tysome JR, Burns E, Whitehead MA, Aksoy E, Alusi G, Hiley C, Ahmed J, Vanhaesebroeck B, Lemoine NR, Wang Y. Transient Inhibition of PI3Kδ Enhances the Therapeutic Effect of Intravenous Delivery of Oncolytic Vaccinia Virus. Mol Ther. 2020 May 6;28(5):1263-1275. doi: 10.1016/j.ymthe.2020.02.017. Epub 2020 Feb 28. PMID: 32145202; PMCID: PMC7210704. 4. Lee HY, Lee GH, Kim HR, Lee YC, Chae HJ. Phosphatidylinositol 3-kinase-δ controls endoplasmic reticulum membrane fluidity and permeability in fungus-induced allergic inflammation in mice. Br J Pharmacol. 2020 Apr;177(7):1556-1567. doi: 10.1111/bph.14917. Epub 2020 Jan 27. PMID: 31713846; PMCID: PMC7060358.
In vitro protocol:
1. Zheng L, Xing L, Zeng C, Wu T, Gui Y, Li W, Lan T, Yang Y, Gu Q, Qi C, Zhang Q, Tang F, He X, Wang L. Inactivation of PI3Kδ induces vascular injury and promotes aneurysm development by upregulating the AP-1/MMP-12 pathway in macrophages. Arterioscler Thromb Vasc Biol. 2015 Feb;35(2):368-77. doi: 10.1161/ATVBAHA.114.304365. Epub 2014 Dec 11. PMID: 25503990. 2. Sadhu C, Masinovsky B, Dick K, Sowell CG, Staunton DE. Essential role of phosphoinositide 3-kinase delta in neutrophil directional movement. J Immunol. 2003 Mar 1;170(5):2647-54. doi: 10.4049/jimmunol.170.5.2647. PMID: 12594293.
In vivo protocol:
1. Ferguson MS, Chard Dunmall LS, Gangeswaran R, Marelli G, Tysome JR, Burns E, Whitehead MA, Aksoy E, Alusi G, Hiley C, Ahmed J, Vanhaesebroeck B, Lemoine NR, Wang Y. Transient Inhibition of PI3Kδ Enhances the Therapeutic Effect of Intravenous Delivery of Oncolytic Vaccinia Virus. Mol Ther. 2020 May 6;28(5):1263-1275. doi: 10.1016/j.ymthe.2020.02.017. Epub 2020 Feb 28. PMID: 32145202; PMCID: PMC7210704. 2. Lee HY, Lee GH, Kim HR, Lee YC, Chae HJ. Phosphatidylinositol 3-kinase-δ controls endoplasmic reticulum membrane fluidity and permeability in fungus-induced allergic inflammation in mice. Br J Pharmacol. 2020 Apr;177(7):1556-1567. doi: 10.1111/bph.14917. Epub 2020 Jan 27. PMID: 31713846; PMCID: PMC7060358.
1: Kappala D, Ramakrishnan S, Nikunjkumar Prakashbhai P, Kaliappan A, Thomas P, Linde J, Singh M, Dey S, Chellappa MM. Resiquimod induces a mixed Th1 and Th2 response via STAT1 and STAT3 signalling in chickens. Biochem Biophys Rep. 2025 Feb 4;41:101941. doi: 10.1016/j.bbrep.2025.101941. PMID: 39990625; PMCID: PMC11847045. 2: Miao X, Jiang Y, Kong D, Wu Z, Liu H, Ye X, Gong W. Lactobacillus rhamnosus HN001 Ameliorates BEZ235-Induced Intestinal Dysbiosis and Prolongs Cardiac Transplant Survival. Microbiol Spectr. 2022 Aug 31;10(4):e0079422. doi: 10.1128/spectrum.00794-22. Epub 2022 Jul 11. PMID: 35862958; PMCID: PMC9430965. 3: Ogawa T, Kan-O K, Shiota A, Fujita A, Ishii Y, Fukuyama S, Matsumoto K. Inhibition of PI3Kδ Differentially Regulates Poly I:C- and Human Metapneumovirus-Induced PD-L1 and PD-L2 Expression in Human Bronchial Epithelial Cells. Front Immunol. 2021 Nov 25;12:767666. doi: 10.3389/fimmu.2021.767666. PMID: 34899719; PMCID: PMC8656419. 4: Yao L, Tang Y, Chen J, Li J, Wang H, Lu M, Gao L, Liu F, Chang P, Liu X, Tang H. Impaired airway epithelial barrier integrity was mediated by PI3Kδ in a mouse model of lipopolysaccharide-induced acute lung injury. Int Immunopharmacol. 2021 Jun;95:107570. doi: 10.1016/j.intimp.2021.107570. Epub 2021 Mar 24. PMID: 33773208. 5: Xu C, Chen S, Deng Y, Song J, Li J, Chen X, Chang P, Yao L, Tang H. Distinct roles of PI3Kδ and PI3Kγ in a toluene diisocyanate-induced murine asthma model. Toxicology. 2021 Apr 30;454:152747. doi: 10.1016/j.tox.2021.152747. Epub 2021 Mar 9. PMID: 33711354. 6: Tran KB, Kolekar S, Jabed A, Jaynes P, Shih JH, Wang Q, Flanagan JU, Rewcastle GW, Baguley BC, Shepherd PR. Diverse mechanisms activate the PI 3-kinase/mTOR pathway in melanomas: implications for the use of PI 3-kinase inhibitors to overcome resistance to inhibitors of BRAF and MEK. BMC Cancer. 2021 Feb 6;21(1):136. doi: 10.1186/s12885-021-07826-4. PMID: 33549048; PMCID: PMC7866738. 7: White AR, Tiwari D, MacLeod MC, Danzer SC, Gross C. PI3K isoform-selective inhibition in neuron-specific PTEN-deficient mice rescues molecular defects and reduces epilepsy-associated phenotypes. Neurobiol Dis. 2020 Oct;144:105026. doi: 10.1016/j.nbd.2020.105026. Epub 2020 Jul 24. PMID: 32712265; PMCID: PMC7484255. 8: Fujita A, Kan-O K, Tonai K, Yamamoto N, Ogawa T, Fukuyama S, Nakanishi Y, Matsumoto K. Inhibition of PI3Kδ Enhances Poly I:C-Induced Antiviral Responses and Inhibits Replication of Human Metapneumovirus in Murine Lungs and Human Bronchial Epithelial Cells. Front Immunol. 2020 Mar 11;11:432. doi: 10.3389/fimmu.2020.00432. PMID: 32218789; PMCID: PMC7079687. 9: Ferguson MS, Chard Dunmall LS, Gangeswaran R, Marelli G, Tysome JR, Burns E, Whitehead MA, Aksoy E, Alusi G, Hiley C, Ahmed J, Vanhaesebroeck B, Lemoine NR, Wang Y. Transient Inhibition of PI3Kδ Enhances the Therapeutic Effect of Intravenous Delivery of Oncolytic Vaccinia Virus. Mol Ther. 2020 May 6;28(5):1263-1275. doi: 10.1016/j.ymthe.2020.02.017. Epub 2020 Feb 28. PMID: 32145202; PMCID: PMC7210704. 10: Gündüz D, Troidl C, Tanislav C, Rohrbach S, Hamm C, Aslam M. Role of PI3K/Akt and MEK/ERK Signalling in cAMP/Epac-Mediated Endothelial Barrier Stabilisation. Front Physiol. 2019 Nov 7;10:1387. doi: 10.3389/fphys.2019.01387. PMID: 31787905; PMCID: PMC6855264. 11: Gupta P, Singh P, Pandey HS, Seth P, Mukhopadhyay CK. Phosphoinositide-3-kinase inhibition elevates ferritin level resulting depletion of labile iron pool and blocking of glioma cell proliferation. Biochim Biophys Acta Gen Subj. 2019 Mar;1863(3):547-564. doi: 10.1016/j.bbagen.2018.12.013. Epub 2018 Dec 23. PMID: 30586625. 12: Wu C, Liu H, Li J, Xiao HP, Li X, Jiang J. Chiral 1H NMR of Atropisomeric Quinazolinones With Enantiopure Phosphoric Acids. Front Chem. 2018 Aug 17;6:300. doi: 10.3389/fchem.2018.00300. PMID: 30175093; PMCID: PMC6107746. 13: Goulielmaki E, Bermudez-Brito M, Andreou M, Tzenaki N, Tzardi M, de Bree E, Tsentelierou E, Makrigiannakis A, Papakonstanti EA. Pharmacological inactivation of the PI3K p110δ prevents breast tumour progression by targeting cancer cells and macrophages. Cell Death Dis. 2018 Jun 7;9(6):678. doi: 10.1038/s41419-018-0717-4. PMID: 29880805; PMCID: PMC5992183. 14: Kim HK, Lee GH, Bhattarai KR, Junjappa RP, Lee HY, Handigund M, Marahatta A, Bhandary B, Baek IH, Pyo JS, Kim HK, Chai OH, Kim HR, Lee YC, Chae HJ. PI3Kδ contributes to ER stress-associated asthma through ER-redox disturbances: the involvement of the RIDD-RIG-I-NF-κB axis. Exp Mol Med. 2018 Feb 16;50(2):e444. doi: 10.1038/emm.2017.270. PMID: 29504610; PMCID: PMC5903822. 15: Jiang Y, Hao S, Tian W, Gao B, Zhang X, Zhang S, Guo L, Yan J, Luo D. PI3K inhibitors IC87114 inhibits the migration and invasion of thyroid cancer cell in vitro and in vivo. J Cell Biochem. 2018 May;119(5):4097-4102. doi: 10.1002/jcb.26604. Epub 2018 Jan 22. PMID: 29236327. 16: El-Hashim AZ, Khajah MA, Renno WM, Babyson RS, Uddin M, Benter IF, Ezeamuzie C, Akhtar S. Src-dependent EGFR transactivation regulates lung inflammation via downstream signaling involving ERK1/2, PI3Kδ/Akt and NFκB induction in a murine asthma model. Sci Rep. 2017 Aug 30;7(1):9919. doi: 10.1038/s41598-017-09349-0. PMID: 28855674; PMCID: PMC5577320. 17: Lodola A, Bertolini S, Biagetti M, Capacchi S, Facchinetti F, Gallo PM, Pappani A, Mor M, Pala D, Rivara S, Visentini F, Corsi M, Capelli AM. Atropisomerism and Conformational Equilibria: Impact on PI3Kδ Inhibition of 2-((6-Amino-9H-purin-9-yl)methyl)-5-methyl-3-(o-tolyl)quinazolin-4(3H)-one (IC87114) and Its Conformationally Restricted Analogs. J Med Chem. 2017 May 25;60(10):4304-4315. doi: 10.1021/acs.jmedchem.7b00247. Epub 2017 May 15. PMID: 28489362. 18: Khadem F, Jia P, Mou Z, Feiz Barazandeh A, Liu D, Keynan Y, Uzonna JE. Pharmacological inhibition of p110δ subunit of PI3K confers protection against experimental leishmaniasis. J Antimicrob Chemother. 2017 Feb;72(2):467-477. doi: 10.1093/jac/dkw448. Epub 2016 Dec 20. PMID: 27999013. 19: Jan WC, Kao MC, Yang CH, Chang YY, Huang CJ. Phosphoinositide 3-Kinase Is Involved in Mediating the Anti-inflammation Effects of Vasopressin. Inflammation. 2017 Apr;40(2):435-441. doi: 10.1007/s10753-016-0489-x. PMID: 27943011. 20: Aragoneses-Fenoll L, Montes-Casado M, Ojeda G, Acosta YY, Herranz J, Martínez S, Blanco-Aparicio C, Criado G, Pastor J, Dianzani U, Portolés P, Rojo JM. ETP-46321, a dual p110α/δ class IA phosphoinositide 3-kinase inhibitor modulates T lymphocyte activation and collagen-induced arthritis. Biochem Pharmacol. 2016 Apr 15;106:56-69. doi: 10.1016/j.bcp.2016.02.005. Epub 2016 Feb 13. PMID: 26883061.