CAM 833 a selective orthosteric inhibitor of the BRCA2-RAD51 interaction (Kd of 366 nM; IC50 of 6 μM). CAM 833 has no significant off-target interactions when screened at 10 μM in the Cerep ExpresSPanel. It inhibits DNA recombinase RAD51-mediated homologous recombination through binding to RAD51 at the same site as the BRCA2 FxxA motif. In cells, CAM 833 suppresses the assembly of RAD51 into damage-induced filaments at the sites of DNA damage. CAM 833 potentiates radiation-induced cytotoxicity and poly-ADP ribose polymerase (PARP)1 inhibitor-induced growth suppression in BRCA2-wild-type cells. The product is metabolically stable, does not significantly inhibit CYP450 enzymes, and is suitable for in vivo investigation.
MedKoo Cat#: 466236
Name: CAM 833
CAS#: 2758364-02-0
Chemical Formula: C26H26ClFN4O5
Exact Mass: 528.1576
Molecular Weight: 528.97
Elemental Analysis: C, 59.04; H, 4.95; Cl, 6.70; F, 3.59; N, 10.59; O, 15.12
The following data is based on the product molecular weight 528.97 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
